Isolation of Enterococcus faecalis pathogenic genes isolated from oral infection by the effect of ionic liquids based on amino acid and its expression by real-time PCR

Enterococcus faecalis is an important factor in nosocomial infections. The aim of this study was the isolation of the pathogenic genes of Enterococcus faecalis in mouth infection and the study of the expression of these genes by real-time PCR. In this study, 60 isolates of E. faecalis were isolated...

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Bibliographic Details
Published in:Folia microbiologica 2022-10, Vol.67 (5), p.777-784
Main Authors: Bonyadi, Parisa, Amini, Kumarss
Format: Article
Language:English
Subjects:
Amino acids
Antibacterial activity
Antiinfectives and antibacterials
Antimicrobial agents
Applied Microbiology
Biofilms
Biomedical and Life Sciences
Cytotoxicity
Dilution
Enterococcus
Enterococcus faecalis
Environmental Engineering/Biotechnology
Gene expression
Genes
Immunology
Ionic liquids
Ions
Life Sciences
Methionine
Microbiology
Minimum inhibitory concentration
Nosocomial infection
Nosocomial infections
Oral infection
Original Article
Real time
Toxicity testing
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Summary:Enterococcus faecalis is an important factor in nosocomial infections. The aim of this study was the isolation of the pathogenic genes of Enterococcus faecalis in mouth infection and the study of the expression of these genes by real-time PCR. In this study, 60 isolates of E. faecalis were isolated from oral infections. The presence and frequency of cyl , hyl , and esp genes and their expression by ionic liquid were evaluated using real-time PCR. MIC was determined by broth dilution method and biofilm production was measured, then biofilm inhibition ability and cytotoxicity test were performed by MTT method. The esp , cyl , and hyl genes were observed in 10, 11, and 2 isolates, respectively. cyl gene with the highest frequency of expression in the treated group was reduced by 1.14% under the influence of ionic liquid with methionine base. The results of MIC and Sub MIC concentrations were obtained with the effect of ionic liquid including 125 and 225 µg/mL, respectively. Amino-acid-based ionic liquids can also reduce biofilm production at sub-MIC concentrations ( P  
ISSN:0015-5632
1874-9356
DOI:10.1007/s12223-022-00972-7