Peptide Nanosponges Designed for the Delivery of Perillyl Alcohol to Glioma Cells

Peptide nanosponges of low polydispersity are spontaneously formed from trigonal supramolecular building blocks in aqueous buffers, which feature cationic and/or anionic oligopeptides (n = 5–20) and a hydrophobic unit. In contrast to classical liposomes/vesicles, nanosponges feature interwoven hydro...

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Published in:ACS applied bio materials 2019-01, Vol.2 (1), p.49-60
Main Authors: Yapa, Asanka S, Shrestha, Tej B, Wendel, Sebastian O, Kalubowilage, Madumali, Yu, Jing, Wang, Hongwang, Pyle, Marla, Basel, Matthew T, Toledo, Yubisela, Ortega, Raquel, Malalasekera, Aruni P, Thapa, Prem S, Troyer, Deryl L, Bossmann, Stefan H
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cited_by cdi_FETCH-LOGICAL-a330t-a173c814ccaad577452b38bd7c79c3380b85aba4d2ef9579e2735549a36cd92d3
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container_start_page 49
container_title ACS applied bio materials
container_volume 2
creator Yapa, Asanka S
Shrestha, Tej B
Wendel, Sebastian O
Kalubowilage, Madumali
Yu, Jing
Wang, Hongwang
Pyle, Marla
Basel, Matthew T
Toledo, Yubisela
Ortega, Raquel
Malalasekera, Aruni P
Thapa, Prem S
Troyer, Deryl L
Bossmann, Stefan H
description Peptide nanosponges of low polydispersity are spontaneously formed from trigonal supramolecular building blocks in aqueous buffers, which feature cationic and/or anionic oligopeptides (n = 5–20) and a hydrophobic unit. In contrast to classical liposomes/vesicles, nanosponges feature interwoven hydrophilic and hydrophobic nanodomains and are readily taken up by mammalian cells. Perillyl alcohol is known to be a simple, but effective small molecule drug against glioma multiforme. However, its efficacy is limited by a poor bioavailability. In order to make perillyl alcohol bioavailable, two nanosponges consisting of 10 aspartates, to which perillyl alcohol is attached by means of an ester bond, and 20 lysines or arginines (type (D-POH)10K20 and (D-POH)10R20) were synthesized, purified, and characterized by dynamic light scattering (DLS) and atomic force microscopy (AFM). These nanosponges were then tested in cell cultures of murine glioma cells (GL26) and murine neural progenitor cells (NPC) because the latter was previously utilized in cell-based cancer therapy. The two nanosponges exhibited significantly different biophysical properties (size distribution and ζ potentials). Consequently, different efficacies in killing GL26 and NPC were observed in serum-containing culture media. The results from these experiments confirmed that the type (D-POH)10K20 nanosponge is a promising candidate for the (cell-mediated) cytotherapy of glioblastoma.
doi_str_mv 10.1021/acsabm.8b00305
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title Peptide Nanosponges Designed for the Delivery of Perillyl Alcohol to Glioma Cells
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