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Reference Genes and Expression Analysis of Seed Desaturases Genes in Soybean Mutant Accessions
Soybean oil is the second most-produced vegetable oil worldwide. To enhance the nutritional quality and oxidative stability of soybean oil, many soybean breeding programs are trying to increase oleic acid content and reduce linoleic and linolenic acid contents. The fatty acid profile of soybean oil...
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Published in: | Biochemical genetics 2022-06, Vol.60 (3), p.937-952 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Soybean oil is the second most-produced vegetable oil worldwide. To enhance the nutritional quality and oxidative stability of soybean oil, many soybean breeding programs are trying to increase oleic acid content and reduce linoleic and linolenic acid contents. The fatty acid profile of soybean oil is controlled by many genes, including those which code for omega-3 and omega-6 desaturases. Mutations in
GmFAD2-1
and
GmFAD3
genes are widely studied and their combinations can produce soybean oil with high oleic and low linoleic and linolenic content. However, few studies evaluate the effect of these mutations on gene expression. Therefore, the present study sought to identify reference genes, evaluate the expression of
GmFAD2-1
and
GmFAD3
seed desaturase genes in thirteen wild-type and mutated soybean accessions, and associate the expression patterns with fatty acid composition and with the
GmFAD2-1
and
GmFAD3
genotypes.
GmCONS7
and
GmUKN2
were identified as the best reference genes for combined use to normalize data. The
GmFAD2-1A
mutation of PI603452 accession was associated with a decrease in gene expression of
GmFAD2-1A
; however, downregulation may not be due to the truncated enzyme structure alone. These results suggested that there are factors other than
GmFAD2-1A
and
GmFAD2-1B
that have a considerable effect on oleic content, at least in soybeans with mutations in these two genes. |
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ISSN: | 0006-2928 1573-4927 |
DOI: | 10.1007/s10528-021-10135-x |