Impact of MtrA on phosphate metabolism genes and the response to altered phosphate conditions in Streptomyces

Summary Phosphate metabolism is known to be regulated by the PhoPR regulatory system in Streptomyces and some other bacteria. In this study, we report that MtrA also regulates phosphate metabolism in Streptomyces. Our data showed that, in Streptomyces coelicolor, MtrA regulates not only phosphate me...

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Bibliographic Details
Published in:Environmental microbiology 2021-11, Vol.23 (11), p.6907-6923
Main Authors: Zhu, Yanping, Zhang, Peipei, Lu, Ting, Wang, Xinyuan, Li, Aiying, Lu, Yinhua, Tao, Meifeng, Pang, Xiuhua
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Complex media
Gene expression
Gene Expression Regulation, Bacterial
Genes
Homology
Metabolism
Phosphate
Phosphates
Phosphates - metabolism
Streptomyces
Streptomyces - genetics
Streptomyces - metabolism
Streptomyces coelicolor - genetics
Streptomyces coelicolor - metabolism
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Summary:Summary Phosphate metabolism is known to be regulated by the PhoPR regulatory system in Streptomyces and some other bacteria. In this study, we report that MtrA also regulates phosphate metabolism in Streptomyces. Our data showed that, in Streptomyces coelicolor, MtrA regulates not only phosphate metabolism genes such as phoA but also phoP under different phosphate conditions, including growth on rich complex media without added inorganic phosphate and on defined media with low or high concentrations of inorganic phosphate. Cross‐regulation was also observed among mtrA, phoP and glnR under these conditions. We demonstrated both in vitro and in vivo binding of MtrA to the promoter regions of genes associated with phosphate metabolism and to the intergenic region between phoR and phoU, indicating that these phosphate metabolism genes are targets of MtrA. We further showed that MtrA in S. lividans and S. venezuelae has detectable regulatory effects on expression of phosphate metabolism genes. Additionally, the MtrA homologue from Corynebacterium glutamicum bound predicted MtrA sites of multiple phosphate metabolism genes, implying its potential for regulating phosphate metabolism in this species. Overall, our findings support MtrA as a major regulator for phosphate metabolism in Streptomyces and also potentially in other actinobacteria.
ISSN:1462-2912
1462-2920
DOI:10.1111/1462-2920.15719