Comparison of the metabolome in urine prior and eight weeks after radical prostatectomy uncovers pathologic and molecular features of prostate cancer

•UPLC-MS-based non-targeted metabolomics was applied in prostate cancer study.•Metabolic characteristics of urine in patients pre- and post-radical prostatectomy were assessed for the first time.•A total of 23 metabolites were found altered significantly before and after surgery.•Fatty acid metaboli...

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Published in:Journal of pharmaceutical and biomedical analysis 2021-10, Vol.205, p.114288-114288, Article 114288
Main Authors: Walz, Simon, Wang, Qingqing, Zhao, Xinjie, Hoene, Miriam, Häring, Hans-Ulrich, Hennenlotter, Jörg, Maas, Moritz, Peter, Andreas, Todenhöfer, Tilman, Stenzl, Arnulf, Liu, Xinyu, Lehmann, Rainer, Xu, Guowang
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Language:English
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Metabolomics
Prostate cancer
Radical prostatectomy
Urine
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creator Walz, Simon
Wang, Qingqing
Zhao, Xinjie
Hoene, Miriam
Häring, Hans-Ulrich
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Stenzl, Arnulf
Liu, Xinyu
Lehmann, Rainer
Xu, Guowang
description •UPLC-MS-based non-targeted metabolomics was applied in prostate cancer study.•Metabolic characteristics of urine in patients pre- and post-radical prostatectomy were assessed for the first time.•A total of 23 metabolites were found altered significantly before and after surgery.•Fatty acid metabolism played an important role in prostate malignancy. Prostate cancer (PCa) is associated with cellular metabolism alterations leading to changes of the metabolome. So far, studies investigating these alterations mainly focused on comparisons of metabolite profiles of PCa patients and healthy controls. In the present study we compared for the first time metabolite profiles in a significant number of paired urine samples collected before and eight weeks after radical prostatectomy (rPX) in 34 patients with PCa. Our comprehensive non-targeted liquid chromatographic-mass spectrometric metabolomics approach covered > 3000 metabolite ion masses. We annotated 23 metabolites showing significant changes eight weeks after rPX. While the levels of uridine and six acylcarnitines in urine were increased before surgery, lower levels were detected for 16 metabolites, like e.g. citrate, phenyl-lactic acid, choline, myo-inositol, emphasizing a relevant pathophysiological role of these biomarkers and the associated metabolic pathways. These results have important implications for potential use of metabolome analyses for detection of prostate cancer and related pathologic and molecular features.
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subjects Metabolomics
Prostate cancer
Radical prostatectomy
Urine
title Comparison of the metabolome in urine prior and eight weeks after radical prostatectomy uncovers pathologic and molecular features of prostate cancer
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