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Transcriptome-wide N6-methyladenosine methylation landscape of coronary artery disease
To reveal transcriptome-wide N6-methyladenosine (m A) methylome of coronary artery disease (CAD). The m A levels of RNA from peripheral blood mononuclear cells measured by colorimetry were significantly decreased in CAD cases. Transcriptome-wide m A methylome profiled by methylated RNA immunoprecipi...
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Published in: | Epigenomics 2021-05, Vol.13 (10), p.793-808 |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | To reveal transcriptome-wide N6-methyladenosine (m
A) methylome of coronary artery disease (CAD).
The m
A levels of RNA from peripheral blood mononuclear cells measured by colorimetry were significantly decreased in CAD cases. Transcriptome-wide m
A methylome profiled by methylated RNA immunoprecipitation sequencing (MeRIP-seq) identified differentially methylated m
A sites within both mRNAs and lncRNAs between CAD and control group.
Bioinformatic analysis indicated that differentially methylated genes were involved in the pathogenesis of atherosclerosis. MeRIP-quantitative real-time PCR assay confirmed the reliability of MeRIP-seq data. Finally, the rat carotid artery balloon injury model was performed to confirm the role of m
A demethylase
in neointima formation.
Our study provided a resource of differentially methylated m
A profile for uncovering m
A biological functions in the pathogenesis of CAD. |
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ISSN: | 1750-1911 1750-192X |
DOI: | 10.2217/epi-2020-0372 |