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Enhancing therapeutic effects and in vivo tracking of adipose tissue-derived mesenchymal stem cells for liver injury using bioorthogonal click chemistry
Adipose tissue-derived mesenchymal stem cell (ADSC)-based therapy is attractive for liver diseases, but the long-term therapeutic outcome is still far from satisfaction due to the low hepatic engraftment efficiency of ADSC transplantation. Herein, we propose a strategy based on liver sinusoidal endo...
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Published in: | Nanoscale 2021-01, Vol.13 (3), p.1813-1822 |
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creator | Liao, Naishun Zhang, Da Wu, Ming Yang, Huanghao Liu, Xiaolong Song, Jibin |
description | Adipose tissue-derived mesenchymal stem cell (ADSC)-based therapy is attractive for liver diseases, but the long-term therapeutic outcome is still far from satisfaction due to the low hepatic engraftment efficiency of ADSC transplantation. Herein, we propose a strategy based on liver sinusoidal endothelial cell (LSEC)-targeting peptide modification and near infrared (NIR) fluorescent probe labeling for enhancing LSEC-barrier-migration ability and in vivo tracking of ADSCs in a liver injury mouse model. RLTRKRGLK (RK), a LSEC-targeted peptide, and indocyanine green (ICG), a FDA approved infrared fluorescent dye, were simultaneously modified on the ADSC surface via a bioorthogonal click reaction. The equipped ADSCs not only exhibited significant binding ability towards LSEC both in vitro and in vivo, but could also be monitored by NIR imaging in vivo. In particular, the RK-modified ADSCs showed remarkable higher hepatic accumulation as compared to unmodified ADSCs, resulting in better therapeutic outcomes. Therefore, this study provides a simple and convenient method for enhancing the homing of transplanted ADSCs to injured liver accompanying with in vivo cell tracking ability, which may shed light on accelerating the clinical translation of the ADSC-based therapy for liver diseases. |
doi_str_mv | 10.1039/d0nr07272a |
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Therefore, this study provides a simple and convenient method for enhancing the homing of transplanted ADSCs to injured liver accompanying with in vivo cell tracking ability, which may shed light on accelerating the clinical translation of the ADSC-based therapy for liver diseases.</description><identifier>ISSN: 2040-3364</identifier><identifier>EISSN: 2040-3372</identifier><identifier>DOI: 10.1039/d0nr07272a</identifier><identifier>PMID: 33433536</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Adipose Tissue ; Animals ; Cell Tracking ; Cells, Cultured ; Chemical reactions ; Chemical synthesis ; Click Chemistry ; Endothelial cells ; Fluorescent dyes ; Fluorescent indicators ; Injuries ; Liver ; Liver diseases ; Mesenchymal Stem Cells ; Mice ; Near infrared radiation ; Peptides ; Stem cells ; Tracking ; Transplantation</subject><ispartof>Nanoscale, 2021-01, Vol.13 (3), p.1813-1822</ispartof><rights>Copyright Royal Society of Chemistry 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-73fccccb05bdd79d33112bb144fa03e6b86b7807328768b36bf3e08990a099b73</citedby><cites>FETCH-LOGICAL-c352t-73fccccb05bdd79d33112bb144fa03e6b86b7807328768b36bf3e08990a099b73</cites><orcidid>0000-0002-2613-1354 ; 0000-0003-4771-5006 ; 0000-0002-3096-4981 ; 0000-0001-8326-4076 ; 0000-0002-2781-4229</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33433536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liao, Naishun</creatorcontrib><creatorcontrib>Zhang, Da</creatorcontrib><creatorcontrib>Wu, Ming</creatorcontrib><creatorcontrib>Yang, Huanghao</creatorcontrib><creatorcontrib>Liu, Xiaolong</creatorcontrib><creatorcontrib>Song, Jibin</creatorcontrib><title>Enhancing therapeutic effects and in vivo tracking of adipose tissue-derived mesenchymal stem cells for liver injury using bioorthogonal click chemistry</title><title>Nanoscale</title><addtitle>Nanoscale</addtitle><description>Adipose tissue-derived mesenchymal stem cell (ADSC)-based therapy is attractive for liver diseases, but the long-term therapeutic outcome is still far from satisfaction due to the low hepatic engraftment efficiency of ADSC transplantation. Herein, we propose a strategy based on liver sinusoidal endothelial cell (LSEC)-targeting peptide modification and near infrared (NIR) fluorescent probe labeling for enhancing LSEC-barrier-migration ability and in vivo tracking of ADSCs in a liver injury mouse model. RLTRKRGLK (RK), a LSEC-targeted peptide, and indocyanine green (ICG), a FDA approved infrared fluorescent dye, were simultaneously modified on the ADSC surface via a bioorthogonal click reaction. The equipped ADSCs not only exhibited significant binding ability towards LSEC both in vitro and in vivo, but could also be monitored by NIR imaging in vivo. In particular, the RK-modified ADSCs showed remarkable higher hepatic accumulation as compared to unmodified ADSCs, resulting in better therapeutic outcomes. Therefore, this study provides a simple and convenient method for enhancing the homing of transplanted ADSCs to injured liver accompanying with in vivo cell tracking ability, which may shed light on accelerating the clinical translation of the ADSC-based therapy for liver diseases.</description><subject>Adipose Tissue</subject><subject>Animals</subject><subject>Cell Tracking</subject><subject>Cells, Cultured</subject><subject>Chemical reactions</subject><subject>Chemical synthesis</subject><subject>Click Chemistry</subject><subject>Endothelial cells</subject><subject>Fluorescent dyes</subject><subject>Fluorescent indicators</subject><subject>Injuries</subject><subject>Liver</subject><subject>Liver diseases</subject><subject>Mesenchymal Stem Cells</subject><subject>Mice</subject><subject>Near infrared radiation</subject><subject>Peptides</subject><subject>Stem cells</subject><subject>Tracking</subject><subject>Transplantation</subject><issn>2040-3364</issn><issn>2040-3372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpd0ctq3TAQBmBRWppLu-kDFEE3JeBG1vhI9jKk6QVCAqVdG13GsU5s6VSSD5w36eNGbtIsOhvN4uPXwE_Iu5p9qhl055b5yCSXXL0gx5w1rAKQ_OXzLpojcpLSljHRgYDX5AigAdiAOCZ_rvyovHH-juYRo9rhkp2hOAxocqLKW-o83bt9oDkqc7_CMFBl3S4kpNmltGBlMbo9WjpjQm_Gw6wmmjLO1OA0JTqESKcCYsnaLvFAl7TmaBdCzGO4C754MzlzT82Is0s5Ht6QV4OaEr59ek_Jry9XPy-_Vde3X79fXlxXBjY8VxIGU0azjbZWdhagrrnWddMMigEK3QotWyaBt1K0GoQeAFnbdUyxrtMSTsnHx9xdDL8XTLkv_69nK49hST1vpOSCtw0r9MN_dBuWWG5fVcs7VkPTFXX2qEwMKUUc-l10s4qHvmb92lf_md38-NvXRcHvnyIXPaN9pv8KggcJI5Mu</recordid><startdate>20210128</startdate><enddate>20210128</enddate><creator>Liao, Naishun</creator><creator>Zhang, Da</creator><creator>Wu, Ming</creator><creator>Yang, Huanghao</creator><creator>Liu, Xiaolong</creator><creator>Song, Jibin</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2613-1354</orcidid><orcidid>https://orcid.org/0000-0003-4771-5006</orcidid><orcidid>https://orcid.org/0000-0002-3096-4981</orcidid><orcidid>https://orcid.org/0000-0001-8326-4076</orcidid><orcidid>https://orcid.org/0000-0002-2781-4229</orcidid></search><sort><creationdate>20210128</creationdate><title>Enhancing therapeutic effects and in vivo tracking of adipose tissue-derived mesenchymal stem cells for liver injury using bioorthogonal click chemistry</title><author>Liao, Naishun ; Zhang, Da ; Wu, Ming ; Yang, Huanghao ; Liu, Xiaolong ; Song, Jibin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-73fccccb05bdd79d33112bb144fa03e6b86b7807328768b36bf3e08990a099b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adipose Tissue</topic><topic>Animals</topic><topic>Cell Tracking</topic><topic>Cells, Cultured</topic><topic>Chemical reactions</topic><topic>Chemical synthesis</topic><topic>Click Chemistry</topic><topic>Endothelial cells</topic><topic>Fluorescent dyes</topic><topic>Fluorescent indicators</topic><topic>Injuries</topic><topic>Liver</topic><topic>Liver diseases</topic><topic>Mesenchymal Stem Cells</topic><topic>Mice</topic><topic>Near infrared radiation</topic><topic>Peptides</topic><topic>Stem cells</topic><topic>Tracking</topic><topic>Transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liao, Naishun</creatorcontrib><creatorcontrib>Zhang, Da</creatorcontrib><creatorcontrib>Wu, Ming</creatorcontrib><creatorcontrib>Yang, Huanghao</creatorcontrib><creatorcontrib>Liu, Xiaolong</creatorcontrib><creatorcontrib>Song, Jibin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Nanoscale</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liao, Naishun</au><au>Zhang, Da</au><au>Wu, Ming</au><au>Yang, Huanghao</au><au>Liu, Xiaolong</au><au>Song, Jibin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancing therapeutic effects and in vivo tracking of adipose tissue-derived mesenchymal stem cells for liver injury using bioorthogonal click chemistry</atitle><jtitle>Nanoscale</jtitle><addtitle>Nanoscale</addtitle><date>2021-01-28</date><risdate>2021</risdate><volume>13</volume><issue>3</issue><spage>1813</spage><epage>1822</epage><pages>1813-1822</pages><issn>2040-3364</issn><eissn>2040-3372</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Adipose tissue-derived mesenchymal stem cell (ADSC)-based therapy is attractive for liver diseases, but the long-term therapeutic outcome is still far from satisfaction due to the low hepatic engraftment efficiency of ADSC transplantation. Herein, we propose a strategy based on liver sinusoidal endothelial cell (LSEC)-targeting peptide modification and near infrared (NIR) fluorescent probe labeling for enhancing LSEC-barrier-migration ability and in vivo tracking of ADSCs in a liver injury mouse model. RLTRKRGLK (RK), a LSEC-targeted peptide, and indocyanine green (ICG), a FDA approved infrared fluorescent dye, were simultaneously modified on the ADSC surface via a bioorthogonal click reaction. The equipped ADSCs not only exhibited significant binding ability towards LSEC both in vitro and in vivo, but could also be monitored by NIR imaging in vivo. In particular, the RK-modified ADSCs showed remarkable higher hepatic accumulation as compared to unmodified ADSCs, resulting in better therapeutic outcomes. Therefore, this study provides a simple and convenient method for enhancing the homing of transplanted ADSCs to injured liver accompanying with in vivo cell tracking ability, which may shed light on accelerating the clinical translation of the ADSC-based therapy for liver diseases.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>33433536</pmid><doi>10.1039/d0nr07272a</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-2613-1354</orcidid><orcidid>https://orcid.org/0000-0003-4771-5006</orcidid><orcidid>https://orcid.org/0000-0002-3096-4981</orcidid><orcidid>https://orcid.org/0000-0001-8326-4076</orcidid><orcidid>https://orcid.org/0000-0002-2781-4229</orcidid></addata></record> |
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subjects | Adipose Tissue Animals Cell Tracking Cells, Cultured Chemical reactions Chemical synthesis Click Chemistry Endothelial cells Fluorescent dyes Fluorescent indicators Injuries Liver Liver diseases Mesenchymal Stem Cells Mice Near infrared radiation Peptides Stem cells Tracking Transplantation |
title | Enhancing therapeutic effects and in vivo tracking of adipose tissue-derived mesenchymal stem cells for liver injury using bioorthogonal click chemistry |
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