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SLPI in periodontal Ligament is not sleepy during biophysical force‐induced tooth movement
Aim We aimed to identify a key molecule that maintains periodontal tissue homeostasis during biophysical force‐induced tooth movement (BTM) by orchestrating alveolar bone (AB) remodelling. Materials and Methods Differential display‐PCR was performed to identify key molecules for BTM in rats. To inve...
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| Published in: | Journal of clinical periodontology 2021-04, Vol.48 (4), p.528-540 |
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| cites | cdi_FETCH-LOGICAL-c3576-b4885a323fa0bf4e1d7c0fe6773f9ac9861694bf0ef767ddac06b4a85a391bf43 |
| container_end_page | 540 |
| container_issue | 4 |
| container_start_page | 528 |
| container_title | Journal of clinical periodontology |
| container_volume | 48 |
| creator | Lee, Su‐Young Moon, Jung‐Sun Yang, Dong‐Wook Yoo, Hong‐Il Jung, Ji‐Yeon Kim, Ok‐Su Kim, Min‐Seok Koh, Jeong‐Tae Chung, Hyun‐Ju Kim, Sun‐Hun |
| description | Aim
We aimed to identify a key molecule that maintains periodontal tissue homeostasis during biophysical force‐induced tooth movement (BTM) by orchestrating alveolar bone (AB) remodelling.
Materials and Methods
Differential display‐PCR was performed to identify key molecules for BTM in rats. To investigate the localization and expression of the identified molecules, immunofluorescence, real‐time RT‐PCR and Western blotting were performed in rats and human periodontal ligament (PDL) cells. Functional test and micro‐CT analysis were performed to examine the in vivo effects of the identified molecules on BTM.
Results
Secretory leucocyte peptidase inhibitor (SLPI) in the PDL was revealed as a key molecule for BTM‐induced AB remodelling. SLPI was enhanced in the PDL under both compression and tension, and downregulated by an adenyl cyclases inhibitor. SLPI induced osteoblastogenic genes including runt‐related transcription factor 2 (Runx2) and synergistically augmented tension‐induced Runx2 expression. SLPI augmented mineralization in PDL cells. SLPI induced osteoclastogenic genes including receptor activator of nuclear factor kappa‐Β ligand (RANKL) and synergistically augmented the compression‐induced RANKL and macrophage colony‐stimulating factor (MCSF) expression. Finally, the in vivo SLPI application into the AB significantly augmented BTM.
Conclusions
SLPI or its inhibitors might serve as a biological target molecule for therapeutic interventions to modulate BTM. |
| doi_str_mv | 10.1111/jcpe.13416 |
| format | article |
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We aimed to identify a key molecule that maintains periodontal tissue homeostasis during biophysical force‐induced tooth movement (BTM) by orchestrating alveolar bone (AB) remodelling.
Materials and Methods
Differential display‐PCR was performed to identify key molecules for BTM in rats. To investigate the localization and expression of the identified molecules, immunofluorescence, real‐time RT‐PCR and Western blotting were performed in rats and human periodontal ligament (PDL) cells. Functional test and micro‐CT analysis were performed to examine the in vivo effects of the identified molecules on BTM.
Results
Secretory leucocyte peptidase inhibitor (SLPI) in the PDL was revealed as a key molecule for BTM‐induced AB remodelling. SLPI was enhanced in the PDL under both compression and tension, and downregulated by an adenyl cyclases inhibitor. SLPI induced osteoblastogenic genes including runt‐related transcription factor 2 (Runx2) and synergistically augmented tension‐induced Runx2 expression. SLPI augmented mineralization in PDL cells. SLPI induced osteoclastogenic genes including receptor activator of nuclear factor kappa‐Β ligand (RANKL) and synergistically augmented the compression‐induced RANKL and macrophage colony‐stimulating factor (MCSF) expression. Finally, the in vivo SLPI application into the AB significantly augmented BTM.
Conclusions
SLPI or its inhibitors might serve as a biological target molecule for therapeutic interventions to modulate BTM.</description><identifier>ISSN: 0303-6979</identifier><identifier>EISSN: 1600-051X</identifier><identifier>DOI: 10.1111/jcpe.13416</identifier><identifier>PMID: 33370451</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Alveolar bone ; Animals ; biophysical force ; Bone remodeling ; Cbfa-1 protein ; Cells, Cultured ; Compression ; Differential display ; Homeostasis ; Immunofluorescence ; Ligaments ; Localization ; Macrophages ; Mineralization ; PDL ; Peptidase ; Periodontal Ligament ; RANK Ligand ; Rats ; Secretory Leukocyte Peptidase Inhibitor ; SLPI ; Therapeutic applications ; tooth movement ; Tooth Movement Techniques ; TRANCE protein ; Western blotting</subject><ispartof>Journal of clinical periodontology, 2021-04, Vol.48 (4), p.528-540</ispartof><rights>2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3576-b4885a323fa0bf4e1d7c0fe6773f9ac9861694bf0ef767ddac06b4a85a391bf43</citedby><cites>FETCH-LOGICAL-c3576-b4885a323fa0bf4e1d7c0fe6773f9ac9861694bf0ef767ddac06b4a85a391bf43</cites><orcidid>0000-0002-7405-471X ; 0000-0003-4951-2587</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjcpe.13416$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjcpe.13416$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33370451$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Su‐Young</creatorcontrib><creatorcontrib>Moon, Jung‐Sun</creatorcontrib><creatorcontrib>Yang, Dong‐Wook</creatorcontrib><creatorcontrib>Yoo, Hong‐Il</creatorcontrib><creatorcontrib>Jung, Ji‐Yeon</creatorcontrib><creatorcontrib>Kim, Ok‐Su</creatorcontrib><creatorcontrib>Kim, Min‐Seok</creatorcontrib><creatorcontrib>Koh, Jeong‐Tae</creatorcontrib><creatorcontrib>Chung, Hyun‐Ju</creatorcontrib><creatorcontrib>Kim, Sun‐Hun</creatorcontrib><title>SLPI in periodontal Ligament is not sleepy during biophysical force‐induced tooth movement</title><title>Journal of clinical periodontology</title><addtitle>J Clin Periodontol</addtitle><description>Aim
We aimed to identify a key molecule that maintains periodontal tissue homeostasis during biophysical force‐induced tooth movement (BTM) by orchestrating alveolar bone (AB) remodelling.
Materials and Methods
Differential display‐PCR was performed to identify key molecules for BTM in rats. To investigate the localization and expression of the identified molecules, immunofluorescence, real‐time RT‐PCR and Western blotting were performed in rats and human periodontal ligament (PDL) cells. Functional test and micro‐CT analysis were performed to examine the in vivo effects of the identified molecules on BTM.
Results
Secretory leucocyte peptidase inhibitor (SLPI) in the PDL was revealed as a key molecule for BTM‐induced AB remodelling. SLPI was enhanced in the PDL under both compression and tension, and downregulated by an adenyl cyclases inhibitor. SLPI induced osteoblastogenic genes including runt‐related transcription factor 2 (Runx2) and synergistically augmented tension‐induced Runx2 expression. SLPI augmented mineralization in PDL cells. SLPI induced osteoclastogenic genes including receptor activator of nuclear factor kappa‐Β ligand (RANKL) and synergistically augmented the compression‐induced RANKL and macrophage colony‐stimulating factor (MCSF) expression. Finally, the in vivo SLPI application into the AB significantly augmented BTM.
Conclusions
SLPI or its inhibitors might serve as a biological target molecule for therapeutic interventions to modulate BTM.</description><subject>Alveolar bone</subject><subject>Animals</subject><subject>biophysical force</subject><subject>Bone remodeling</subject><subject>Cbfa-1 protein</subject><subject>Cells, Cultured</subject><subject>Compression</subject><subject>Differential display</subject><subject>Homeostasis</subject><subject>Immunofluorescence</subject><subject>Ligaments</subject><subject>Localization</subject><subject>Macrophages</subject><subject>Mineralization</subject><subject>PDL</subject><subject>Peptidase</subject><subject>Periodontal Ligament</subject><subject>RANK Ligand</subject><subject>Rats</subject><subject>Secretory Leukocyte Peptidase Inhibitor</subject><subject>SLPI</subject><subject>Therapeutic applications</subject><subject>tooth movement</subject><subject>Tooth Movement Techniques</subject><subject>TRANCE protein</subject><subject>Western blotting</subject><issn>0303-6979</issn><issn>1600-051X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kMtKxDAUhoMoOl42PoAE3IhQTSZtMl3KMN4YUFDBhRDS9EQztE1NWmV2PoLP6JOYcdSFCw_kZPP9H4cfoV1Kjmic45lu4YiylPIVNKCckIRk9H4VDQgjLOG5yDfQZggzQqhgjK2jjbgFSTM6QA830-sLbBvcgreudE2nKjy1j6qGpsM24MZ1OFQA7RyXvbfNIy6sa5_mwepIGuc1fLy926bsNZS4c657wrV7gUV-G60ZVQXY-f630N3p5HZ8nkyvzi7GJ9NEs0zwpEhHo0yxITOKFCYFWgpNDHAhmMmVzkec8jwtDAEjuChLpQkvUrXI5DQG2BY6WHpb7557CJ2sbdBQVaoB1wc5TAUT8Y1ERPf_oDPX-yZeJ4cZzdgw4zSP1OGS0t6F4MHI1tta-bmkRC46l4vO5VfnEd77VvZFDeUv-lNyBOgSeLUVzP9Rycvx9WQp_QSVoI1J</recordid><startdate>202104</startdate><enddate>202104</enddate><creator>Lee, Su‐Young</creator><creator>Moon, Jung‐Sun</creator><creator>Yang, Dong‐Wook</creator><creator>Yoo, Hong‐Il</creator><creator>Jung, Ji‐Yeon</creator><creator>Kim, Ok‐Su</creator><creator>Kim, Min‐Seok</creator><creator>Koh, Jeong‐Tae</creator><creator>Chung, Hyun‐Ju</creator><creator>Kim, Sun‐Hun</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7405-471X</orcidid><orcidid>https://orcid.org/0000-0003-4951-2587</orcidid></search><sort><creationdate>202104</creationdate><title>SLPI in periodontal Ligament is not sleepy during biophysical force‐induced tooth movement</title><author>Lee, Su‐Young ; Moon, Jung‐Sun ; Yang, Dong‐Wook ; Yoo, Hong‐Il ; Jung, Ji‐Yeon ; Kim, Ok‐Su ; Kim, Min‐Seok ; Koh, Jeong‐Tae ; Chung, Hyun‐Ju ; Kim, Sun‐Hun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3576-b4885a323fa0bf4e1d7c0fe6773f9ac9861694bf0ef767ddac06b4a85a391bf43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alveolar bone</topic><topic>Animals</topic><topic>biophysical force</topic><topic>Bone remodeling</topic><topic>Cbfa-1 protein</topic><topic>Cells, Cultured</topic><topic>Compression</topic><topic>Differential display</topic><topic>Homeostasis</topic><topic>Immunofluorescence</topic><topic>Ligaments</topic><topic>Localization</topic><topic>Macrophages</topic><topic>Mineralization</topic><topic>PDL</topic><topic>Peptidase</topic><topic>Periodontal Ligament</topic><topic>RANK Ligand</topic><topic>Rats</topic><topic>Secretory Leukocyte Peptidase Inhibitor</topic><topic>SLPI</topic><topic>Therapeutic applications</topic><topic>tooth movement</topic><topic>Tooth Movement Techniques</topic><topic>TRANCE protein</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Su‐Young</creatorcontrib><creatorcontrib>Moon, Jung‐Sun</creatorcontrib><creatorcontrib>Yang, Dong‐Wook</creatorcontrib><creatorcontrib>Yoo, Hong‐Il</creatorcontrib><creatorcontrib>Jung, Ji‐Yeon</creatorcontrib><creatorcontrib>Kim, Ok‐Su</creatorcontrib><creatorcontrib>Kim, Min‐Seok</creatorcontrib><creatorcontrib>Koh, Jeong‐Tae</creatorcontrib><creatorcontrib>Chung, Hyun‐Ju</creatorcontrib><creatorcontrib>Kim, Sun‐Hun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of clinical periodontology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Su‐Young</au><au>Moon, Jung‐Sun</au><au>Yang, Dong‐Wook</au><au>Yoo, Hong‐Il</au><au>Jung, Ji‐Yeon</au><au>Kim, Ok‐Su</au><au>Kim, Min‐Seok</au><au>Koh, Jeong‐Tae</au><au>Chung, Hyun‐Ju</au><au>Kim, Sun‐Hun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SLPI in periodontal Ligament is not sleepy during biophysical force‐induced tooth movement</atitle><jtitle>Journal of clinical periodontology</jtitle><addtitle>J Clin Periodontol</addtitle><date>2021-04</date><risdate>2021</risdate><volume>48</volume><issue>4</issue><spage>528</spage><epage>540</epage><pages>528-540</pages><issn>0303-6979</issn><eissn>1600-051X</eissn><notes>This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2019R1A2C1003520) and (No. 2019R1A5A2027521), and Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (2018R1D1A1B07050355).</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Aim
We aimed to identify a key molecule that maintains periodontal tissue homeostasis during biophysical force‐induced tooth movement (BTM) by orchestrating alveolar bone (AB) remodelling.
Materials and Methods
Differential display‐PCR was performed to identify key molecules for BTM in rats. To investigate the localization and expression of the identified molecules, immunofluorescence, real‐time RT‐PCR and Western blotting were performed in rats and human periodontal ligament (PDL) cells. Functional test and micro‐CT analysis were performed to examine the in vivo effects of the identified molecules on BTM.
Results
Secretory leucocyte peptidase inhibitor (SLPI) in the PDL was revealed as a key molecule for BTM‐induced AB remodelling. SLPI was enhanced in the PDL under both compression and tension, and downregulated by an adenyl cyclases inhibitor. SLPI induced osteoblastogenic genes including runt‐related transcription factor 2 (Runx2) and synergistically augmented tension‐induced Runx2 expression. SLPI augmented mineralization in PDL cells. SLPI induced osteoclastogenic genes including receptor activator of nuclear factor kappa‐Β ligand (RANKL) and synergistically augmented the compression‐induced RANKL and macrophage colony‐stimulating factor (MCSF) expression. Finally, the in vivo SLPI application into the AB significantly augmented BTM.
Conclusions
SLPI or its inhibitors might serve as a biological target molecule for therapeutic interventions to modulate BTM.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>33370451</pmid><doi>10.1111/jcpe.13416</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-7405-471X</orcidid><orcidid>https://orcid.org/0000-0003-4951-2587</orcidid></addata></record> |
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| ispartof | Journal of clinical periodontology, 2021-04, Vol.48 (4), p.528-540 |
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| source | Wiley |
| subjects | Alveolar bone Animals biophysical force Bone remodeling Cbfa-1 protein Cells, Cultured Compression Differential display Homeostasis Immunofluorescence Ligaments Localization Macrophages Mineralization PDL Peptidase Periodontal Ligament RANK Ligand Rats Secretory Leukocyte Peptidase Inhibitor SLPI Therapeutic applications tooth movement Tooth Movement Techniques TRANCE protein Western blotting |
| title | SLPI in periodontal Ligament is not sleepy during biophysical force‐induced tooth movement |
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