Loading…
Frail older adults show a distinct plasma microvesicle profile suggesting a prothrombotic and proinflammatory phenotype
In a global context of advanced aging, geriatric diseases such as frailty syndrome face challenges in the search for biomarkers and preventive strategies. Frailty has been associated with atherothrombotic pathologies. Circulating microvesicles (cMVs), phospholipid‐rich vesicles with a size of 0.1–1....
Saved in:
| Published in: | Journal of cellular physiology 2021-03, Vol.236 (3), p.2099-2108 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c3536-9dd2bed9cf044946beaa1ebd602d4f779edacbfcd5780ad19d65e8199d1a1ab83 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c3536-9dd2bed9cf044946beaa1ebd602d4f779edacbfcd5780ad19d65e8199d1a1ab83 |
| container_end_page | 2108 |
| container_issue | 3 |
| container_start_page | 2099 |
| container_title | Journal of cellular physiology |
| container_volume | 236 |
| creator | Arauna, Diego Chiva‐Blanch, Gemma Padró, Teresa Fuentes, Eduardo Palomo, Iván Badimon, Lina |
| description | In a global context of advanced aging, geriatric diseases such as frailty syndrome face challenges in the search for biomarkers and preventive strategies. Frailty has been associated with atherothrombotic pathologies. Circulating microvesicles (cMVs), phospholipid‐rich vesicles with a size of 0.1–1.0 μm, have been shown to participate in atherothrombosis onset and progression. We have hypothesized that cMVs from platelets, and vascular and immune cells, are increased in frail older adults. To verify this, a prevalent‐case control study was designed with 28 frail older and 27 nonfrail older adults older than 64 years. Frailty was defined by Fried's phenotype. Total cMVs, annexin V positive (AV+)‐cMVs, and annexin V negative (AV−)‐cMVs derived from blood and vascular cells were measured by flow cytometry. In the analysis of total cMVs, the frail group presented higher levels of CD14+/CD142+ (p = .042), CD41a+/CD142+ (p = .041), and CD56+ (p = .025), CD14+cMVs (p = .043), and CD16+/CD14+ (p = .019) cMVs levels. Within the phosphatidylserine‐exposing cMVs (AV+), the frail group showed higher CD14+/AV+ (p = .044), CD9+/AV+ (p = .031), P2RY12+/AV+ (p = .028), and CD235a+/AV+ (p = .043) cMVs concentrations. Finally, within AV− cMVs, the frail group showed higher CD142+/CD41a+/AV− cMVs concentrations originated from platelets (p = .027), CD56+/AV− originated from natural killer cells (p = .022), and CD34+/AV− cMVs from hematopoietic stem cells (p = .037). In summary, frail older adults present higher concentrations of platelet‐, leukocyte‐, and hematopoietic cell‐derived cMVs compared to robust age‐matched older adults. These cMVs may be involved in the deregulation of the immune system, endothelial damage, and increased risk of thrombosis associated with frailty.
(a) In the present study, we show for the first time a complete study about the production profile of circulating microvesicles (cMVs) from circulating cells in frail older adults, according to Fried's criteria. (b) We provide evidence that frail older adults present higher concentrations of platelet‐, leukocyte‐, and hematopoietic cell‐derived cMVs compared to robust age‐matched older adults. (c) The findings from this study provide a better understanding of the potential role of the cMVs production in the deregulation of the immune system, endothelial damage, and increased risk of thrombosis associated with frail older adults. |
| doi_str_mv | 10.1002/jcp.29996 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2430377109</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2471744449</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3536-9dd2bed9cf044946beaa1ebd602d4f779edacbfcd5780ad19d65e8199d1a1ab83</originalsourceid><addsrcrecordid>eNp1kc1OwzAQhC0EglI48ALIEhc4BOzEiesjqig_QoIDnKON7bSunDjYCVXfHpcWDkj4stLu59FoBqEzSq4pIenNUnbXqRCi2EMjSgRPWJGn-2gUbzQROaNH6DiEJSFEiCw7REdZypngLB-h1cyDsdhZpT0GNdg-4LBwKwxYmdCbVva4sxAawI2R3n3qYKTVuPOuNnGGYT7XG24ef8Rlv_CuqVxvJIZWbTamrS00DfTOr3G30K3r150-QQc12KBPd3OM3md3b9OH5Pnl_nF6-5zILM-KRCiVVloJWRPGBCsqDUB1pQqSKlZzLrQCWdVS5XxCQFGhilxPqBCKAoVqko3R5VY3OvkYotOyMUFqa6HVbghlyjKScR5Ti-jFH3TpBt9Gd5HilLP4NtTVlophhOB1XXbeNODXJSXlpo0ytlF-txHZ853iUDVa_ZI_8UfgZgusYpbr_5XKp-nrVvILiseXbQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2471744449</pqid></control><display><type>article</type><title>Frail older adults show a distinct plasma microvesicle profile suggesting a prothrombotic and proinflammatory phenotype</title><source>Wiley-Blackwell Journals</source><creator>Arauna, Diego ; Chiva‐Blanch, Gemma ; Padró, Teresa ; Fuentes, Eduardo ; Palomo, Iván ; Badimon, Lina</creator><creatorcontrib>Arauna, Diego ; Chiva‐Blanch, Gemma ; Padró, Teresa ; Fuentes, Eduardo ; Palomo, Iván ; Badimon, Lina</creatorcontrib><description>In a global context of advanced aging, geriatric diseases such as frailty syndrome face challenges in the search for biomarkers and preventive strategies. Frailty has been associated with atherothrombotic pathologies. Circulating microvesicles (cMVs), phospholipid‐rich vesicles with a size of 0.1–1.0 μm, have been shown to participate in atherothrombosis onset and progression. We have hypothesized that cMVs from platelets, and vascular and immune cells, are increased in frail older adults. To verify this, a prevalent‐case control study was designed with 28 frail older and 27 nonfrail older adults older than 64 years. Frailty was defined by Fried's phenotype. Total cMVs, annexin V positive (AV+)‐cMVs, and annexin V negative (AV−)‐cMVs derived from blood and vascular cells were measured by flow cytometry. In the analysis of total cMVs, the frail group presented higher levels of CD14+/CD142+ (p = .042), CD41a+/CD142+ (p = .041), and CD56+ (p = .025), CD14+cMVs (p = .043), and CD16+/CD14+ (p = .019) cMVs levels. Within the phosphatidylserine‐exposing cMVs (AV+), the frail group showed higher CD14+/AV+ (p = .044), CD9+/AV+ (p = .031), P2RY12+/AV+ (p = .028), and CD235a+/AV+ (p = .043) cMVs concentrations. Finally, within AV− cMVs, the frail group showed higher CD142+/CD41a+/AV− cMVs concentrations originated from platelets (p = .027), CD56+/AV− originated from natural killer cells (p = .022), and CD34+/AV− cMVs from hematopoietic stem cells (p = .037). In summary, frail older adults present higher concentrations of platelet‐, leukocyte‐, and hematopoietic cell‐derived cMVs compared to robust age‐matched older adults. These cMVs may be involved in the deregulation of the immune system, endothelial damage, and increased risk of thrombosis associated with frailty.
(a) In the present study, we show for the first time a complete study about the production profile of circulating microvesicles (cMVs) from circulating cells in frail older adults, according to Fried's criteria. (b) We provide evidence that frail older adults present higher concentrations of platelet‐, leukocyte‐, and hematopoietic cell‐derived cMVs compared to robust age‐matched older adults. (c) The findings from this study provide a better understanding of the potential role of the cMVs production in the deregulation of the immune system, endothelial damage, and increased risk of thrombosis associated with frail older adults.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.29996</identifier><identifier>PMID: 32749745</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Adults ; Age related diseases ; Aging ; Aging (natural) ; Annexin V ; Biomarkers ; CD14 antigen ; CD16 antigen ; CD34 antigen ; CD56 antigen ; CD9 antigen ; Deregulation ; Flow cytometry ; Frailty ; frailty syndrome ; Hematopoietic stem cells ; Immune system ; Inflammation ; Leukocytes ; microvesicles ; Natural killer cells ; older adults ; Older people ; Phenotypes ; Phosphatidylserine ; Phospholipids ; Platelets ; Stem cells ; Thromboembolism ; Thrombosis</subject><ispartof>Journal of cellular physiology, 2021-03, Vol.236 (3), p.2099-2108</ispartof><rights>2020 Wiley Periodicals LLC</rights><rights>2020 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3536-9dd2bed9cf044946beaa1ebd602d4f779edacbfcd5780ad19d65e8199d1a1ab83</citedby><cites>FETCH-LOGICAL-c3536-9dd2bed9cf044946beaa1ebd602d4f779edacbfcd5780ad19d65e8199d1a1ab83</cites><orcidid>0000-0003-0099-4108 ; 0000-0002-9162-2459 ; 0000-0003-1921-954X ; 0000-0001-6093-0160 ; 0000-0001-7132-991X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.29996$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.29996$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32749745$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arauna, Diego</creatorcontrib><creatorcontrib>Chiva‐Blanch, Gemma</creatorcontrib><creatorcontrib>Padró, Teresa</creatorcontrib><creatorcontrib>Fuentes, Eduardo</creatorcontrib><creatorcontrib>Palomo, Iván</creatorcontrib><creatorcontrib>Badimon, Lina</creatorcontrib><title>Frail older adults show a distinct plasma microvesicle profile suggesting a prothrombotic and proinflammatory phenotype</title><title>Journal of cellular physiology</title><addtitle>J Cell Physiol</addtitle><description>In a global context of advanced aging, geriatric diseases such as frailty syndrome face challenges in the search for biomarkers and preventive strategies. Frailty has been associated with atherothrombotic pathologies. Circulating microvesicles (cMVs), phospholipid‐rich vesicles with a size of 0.1–1.0 μm, have been shown to participate in atherothrombosis onset and progression. We have hypothesized that cMVs from platelets, and vascular and immune cells, are increased in frail older adults. To verify this, a prevalent‐case control study was designed with 28 frail older and 27 nonfrail older adults older than 64 years. Frailty was defined by Fried's phenotype. Total cMVs, annexin V positive (AV+)‐cMVs, and annexin V negative (AV−)‐cMVs derived from blood and vascular cells were measured by flow cytometry. In the analysis of total cMVs, the frail group presented higher levels of CD14+/CD142+ (p = .042), CD41a+/CD142+ (p = .041), and CD56+ (p = .025), CD14+cMVs (p = .043), and CD16+/CD14+ (p = .019) cMVs levels. Within the phosphatidylserine‐exposing cMVs (AV+), the frail group showed higher CD14+/AV+ (p = .044), CD9+/AV+ (p = .031), P2RY12+/AV+ (p = .028), and CD235a+/AV+ (p = .043) cMVs concentrations. Finally, within AV− cMVs, the frail group showed higher CD142+/CD41a+/AV− cMVs concentrations originated from platelets (p = .027), CD56+/AV− originated from natural killer cells (p = .022), and CD34+/AV− cMVs from hematopoietic stem cells (p = .037). In summary, frail older adults present higher concentrations of platelet‐, leukocyte‐, and hematopoietic cell‐derived cMVs compared to robust age‐matched older adults. These cMVs may be involved in the deregulation of the immune system, endothelial damage, and increased risk of thrombosis associated with frailty.
(a) In the present study, we show for the first time a complete study about the production profile of circulating microvesicles (cMVs) from circulating cells in frail older adults, according to Fried's criteria. (b) We provide evidence that frail older adults present higher concentrations of platelet‐, leukocyte‐, and hematopoietic cell‐derived cMVs compared to robust age‐matched older adults. (c) The findings from this study provide a better understanding of the potential role of the cMVs production in the deregulation of the immune system, endothelial damage, and increased risk of thrombosis associated with frail older adults.</description><subject>Adults</subject><subject>Age related diseases</subject><subject>Aging</subject><subject>Aging (natural)</subject><subject>Annexin V</subject><subject>Biomarkers</subject><subject>CD14 antigen</subject><subject>CD16 antigen</subject><subject>CD34 antigen</subject><subject>CD56 antigen</subject><subject>CD9 antigen</subject><subject>Deregulation</subject><subject>Flow cytometry</subject><subject>Frailty</subject><subject>frailty syndrome</subject><subject>Hematopoietic stem cells</subject><subject>Immune system</subject><subject>Inflammation</subject><subject>Leukocytes</subject><subject>microvesicles</subject><subject>Natural killer cells</subject><subject>older adults</subject><subject>Older people</subject><subject>Phenotypes</subject><subject>Phosphatidylserine</subject><subject>Phospholipids</subject><subject>Platelets</subject><subject>Stem cells</subject><subject>Thromboembolism</subject><subject>Thrombosis</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kc1OwzAQhC0EglI48ALIEhc4BOzEiesjqig_QoIDnKON7bSunDjYCVXfHpcWDkj4stLu59FoBqEzSq4pIenNUnbXqRCi2EMjSgRPWJGn-2gUbzQROaNH6DiEJSFEiCw7REdZypngLB-h1cyDsdhZpT0GNdg-4LBwKwxYmdCbVva4sxAawI2R3n3qYKTVuPOuNnGGYT7XG24ef8Rlv_CuqVxvJIZWbTamrS00DfTOr3G30K3r150-QQc12KBPd3OM3md3b9OH5Pnl_nF6-5zILM-KRCiVVloJWRPGBCsqDUB1pQqSKlZzLrQCWdVS5XxCQFGhilxPqBCKAoVqko3R5VY3OvkYotOyMUFqa6HVbghlyjKScR5Ti-jFH3TpBt9Gd5HilLP4NtTVlophhOB1XXbeNODXJSXlpo0ytlF-txHZ853iUDVa_ZI_8UfgZgusYpbr_5XKp-nrVvILiseXbQ</recordid><startdate>202103</startdate><enddate>202103</enddate><creator>Arauna, Diego</creator><creator>Chiva‐Blanch, Gemma</creator><creator>Padró, Teresa</creator><creator>Fuentes, Eduardo</creator><creator>Palomo, Iván</creator><creator>Badimon, Lina</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0099-4108</orcidid><orcidid>https://orcid.org/0000-0002-9162-2459</orcidid><orcidid>https://orcid.org/0000-0003-1921-954X</orcidid><orcidid>https://orcid.org/0000-0001-6093-0160</orcidid><orcidid>https://orcid.org/0000-0001-7132-991X</orcidid></search><sort><creationdate>202103</creationdate><title>Frail older adults show a distinct plasma microvesicle profile suggesting a prothrombotic and proinflammatory phenotype</title><author>Arauna, Diego ; Chiva‐Blanch, Gemma ; Padró, Teresa ; Fuentes, Eduardo ; Palomo, Iván ; Badimon, Lina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3536-9dd2bed9cf044946beaa1ebd602d4f779edacbfcd5780ad19d65e8199d1a1ab83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adults</topic><topic>Age related diseases</topic><topic>Aging</topic><topic>Aging (natural)</topic><topic>Annexin V</topic><topic>Biomarkers</topic><topic>CD14 antigen</topic><topic>CD16 antigen</topic><topic>CD34 antigen</topic><topic>CD56 antigen</topic><topic>CD9 antigen</topic><topic>Deregulation</topic><topic>Flow cytometry</topic><topic>Frailty</topic><topic>frailty syndrome</topic><topic>Hematopoietic stem cells</topic><topic>Immune system</topic><topic>Inflammation</topic><topic>Leukocytes</topic><topic>microvesicles</topic><topic>Natural killer cells</topic><topic>older adults</topic><topic>Older people</topic><topic>Phenotypes</topic><topic>Phosphatidylserine</topic><topic>Phospholipids</topic><topic>Platelets</topic><topic>Stem cells</topic><topic>Thromboembolism</topic><topic>Thrombosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arauna, Diego</creatorcontrib><creatorcontrib>Chiva‐Blanch, Gemma</creatorcontrib><creatorcontrib>Padró, Teresa</creatorcontrib><creatorcontrib>Fuentes, Eduardo</creatorcontrib><creatorcontrib>Palomo, Iván</creatorcontrib><creatorcontrib>Badimon, Lina</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arauna, Diego</au><au>Chiva‐Blanch, Gemma</au><au>Padró, Teresa</au><au>Fuentes, Eduardo</au><au>Palomo, Iván</au><au>Badimon, Lina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Frail older adults show a distinct plasma microvesicle profile suggesting a prothrombotic and proinflammatory phenotype</atitle><jtitle>Journal of cellular physiology</jtitle><addtitle>J Cell Physiol</addtitle><date>2021-03</date><risdate>2021</risdate><volume>236</volume><issue>3</issue><spage>2099</spage><epage>2108</epage><pages>2099-2108</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><notes>Diego Arauna and Gemma Chiva‐Blanch contributed equally to this work.</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>In a global context of advanced aging, geriatric diseases such as frailty syndrome face challenges in the search for biomarkers and preventive strategies. Frailty has been associated with atherothrombotic pathologies. Circulating microvesicles (cMVs), phospholipid‐rich vesicles with a size of 0.1–1.0 μm, have been shown to participate in atherothrombosis onset and progression. We have hypothesized that cMVs from platelets, and vascular and immune cells, are increased in frail older adults. To verify this, a prevalent‐case control study was designed with 28 frail older and 27 nonfrail older adults older than 64 years. Frailty was defined by Fried's phenotype. Total cMVs, annexin V positive (AV+)‐cMVs, and annexin V negative (AV−)‐cMVs derived from blood and vascular cells were measured by flow cytometry. In the analysis of total cMVs, the frail group presented higher levels of CD14+/CD142+ (p = .042), CD41a+/CD142+ (p = .041), and CD56+ (p = .025), CD14+cMVs (p = .043), and CD16+/CD14+ (p = .019) cMVs levels. Within the phosphatidylserine‐exposing cMVs (AV+), the frail group showed higher CD14+/AV+ (p = .044), CD9+/AV+ (p = .031), P2RY12+/AV+ (p = .028), and CD235a+/AV+ (p = .043) cMVs concentrations. Finally, within AV− cMVs, the frail group showed higher CD142+/CD41a+/AV− cMVs concentrations originated from platelets (p = .027), CD56+/AV− originated from natural killer cells (p = .022), and CD34+/AV− cMVs from hematopoietic stem cells (p = .037). In summary, frail older adults present higher concentrations of platelet‐, leukocyte‐, and hematopoietic cell‐derived cMVs compared to robust age‐matched older adults. These cMVs may be involved in the deregulation of the immune system, endothelial damage, and increased risk of thrombosis associated with frailty.
(a) In the present study, we show for the first time a complete study about the production profile of circulating microvesicles (cMVs) from circulating cells in frail older adults, according to Fried's criteria. (b) We provide evidence that frail older adults present higher concentrations of platelet‐, leukocyte‐, and hematopoietic cell‐derived cMVs compared to robust age‐matched older adults. (c) The findings from this study provide a better understanding of the potential role of the cMVs production in the deregulation of the immune system, endothelial damage, and increased risk of thrombosis associated with frail older adults.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>32749745</pmid><doi>10.1002/jcp.29996</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-0099-4108</orcidid><orcidid>https://orcid.org/0000-0002-9162-2459</orcidid><orcidid>https://orcid.org/0000-0003-1921-954X</orcidid><orcidid>https://orcid.org/0000-0001-6093-0160</orcidid><orcidid>https://orcid.org/0000-0001-7132-991X</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9541 |
| ispartof | Journal of cellular physiology, 2021-03, Vol.236 (3), p.2099-2108 |
| issn | 0021-9541 1097-4652 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2430377109 |
| source | Wiley-Blackwell Journals |
| subjects | Adults Age related diseases Aging Aging (natural) Annexin V Biomarkers CD14 antigen CD16 antigen CD34 antigen CD56 antigen CD9 antigen Deregulation Flow cytometry Frailty frailty syndrome Hematopoietic stem cells Immune system Inflammation Leukocytes microvesicles Natural killer cells older adults Older people Phenotypes Phosphatidylserine Phospholipids Platelets Stem cells Thromboembolism Thrombosis |
| title | Frail older adults show a distinct plasma microvesicle profile suggesting a prothrombotic and proinflammatory phenotype |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T13%3A37%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Frail%20older%20adults%20show%20a%20distinct%20plasma%20microvesicle%20profile%20suggesting%20a%20prothrombotic%20and%20proinflammatory%20phenotype&rft.jtitle=Journal%20of%20cellular%20physiology&rft.au=Arauna,%20Diego&rft.date=2021-03&rft.volume=236&rft.issue=3&rft.spage=2099&rft.epage=2108&rft.pages=2099-2108&rft.issn=0021-9541&rft.eissn=1097-4652&rft_id=info:doi/10.1002/jcp.29996&rft_dat=%3Cproquest_cross%3E2471744449%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c3536-9dd2bed9cf044946beaa1ebd602d4f779edacbfcd5780ad19d65e8199d1a1ab83%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2471744449&rft_id=info:pmid/32749745&rfr_iscdi=true |