Evaluation of filter paper as a means to transport inactivated bacteria for identification using the MALDI-TOF MS system

A novel approach to transport inactivated bacteria in filter paper for identification in the MALDI-TOF MS was evaluated. Seventy four bacterial isolates were evaluated and the approach presented sensitivity of 97.3% and specificity of 100%. Inactivated bacteria in filter paper are a safer alternativ...

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Bibliographic Details
Published in:Journal of microbiological methods 2020-04, Vol.171, p.105863-105863, Article 105863
Main Authors: Carneiro, M.S., Fracasso, A., Lovison, O.V.A., Barreto, F., Barth, A.L.
Format: Article
Language:English
Subjects:
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacterial Typing Techniques - methods
Filter paper
MALDI-TOF MS
Specimen Handling - methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Transport bacteria
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Summary:A novel approach to transport inactivated bacteria in filter paper for identification in the MALDI-TOF MS was evaluated. Seventy four bacterial isolates were evaluated and the approach presented sensitivity of 97.3% and specificity of 100%. Inactivated bacteria in filter paper are a safer alternative to transport bacteria for MALDI-TOF MS identification. •Inactive bacteria impregnated on filter paper reduces biological risks.•MALDI-TOF/MS system was able to identify bacteria impregnated on filter paper.•This is a simple and low cost alternative to transport bacteria for identification.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2020.105863