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Distribution and relevance of Dientamoeba fragilis and Blastocystis species in gastroenteritis: results from a case-control study
The actual role of Dientamoeba fragilis and Blastocystis in patients with gastrointestinal symptoms is still under debate. A multicenter case-control study was performed in The Netherlands to elucidate the clinical relevance of molecular diagnostics results in gastroenteritis (GE). Samples from this...
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Published in: | European journal of clinical microbiology & infectious diseases 2020, Vol.39 (1), p.197-203 |
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creator | de Boer, Maria Dullaert Schuurs, Theo A. Vermeer, Marloes Ruijs, Gijs J.H.M. van der Zanden, Adri G. M. Weel, Jan F. Bruijnesteijn van Coppenraet, Lesla E.S. |
description | The actual role of
Dientamoeba fragilis
and
Blastocystis
in patients with gastrointestinal symptoms is still under debate. A multicenter case-control study was performed in The Netherlands to elucidate the clinical relevance of molecular diagnostics results in gastroenteritis (GE). Samples from this case-control study were used to perform a detailed analysis on the presence of
D. fragilis
and
Blastocystis
in relation to gastrointestinal symptoms. In the present study, a real-time PCR for
Blastocystis
was performed on 1374 case samples and 1026 control samples from the multicenter gastroenteritis case-control study previously tested for
D. fragilis
. Prevalence of both micro-organisms was highest in children under 20 years of age and lowest in the oldest age group. A significantly lower overall detection of
D. fragilis
and
Blastocystis
was found in cases (both 25.8%) as compared to controls (37.6% and 40.0%, respectively). The difference for
D. fragilis
was statistically significant for subjects above 20 years of age. For
Blastocystis
, the difference was statistically significant in all age groups, except in children less than 5 years of age. A negative relation between
D. fragilis
-positive cases and diarrhea was found in this study population. More GE symptoms were reported in cases without
D. fragilis
or
Blastocystis
. In the present study, prevalence of both
D. fragilis
and
Blastocystis
is lower in cases with gastroenteritic symptoms than in controls. Besides, in cases with
D. fragilis
or
Blastocystis
, no association is shown between any of the GE symptoms. Interestingly, this suggests that the presence of these protozoans may be considered characteristic of a healthy intestinal microbiome. |
doi_str_mv | 10.1007/s10096-019-03710-z |
format | article |
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Dientamoeba fragilis
and
Blastocystis
in patients with gastrointestinal symptoms is still under debate. A multicenter case-control study was performed in The Netherlands to elucidate the clinical relevance of molecular diagnostics results in gastroenteritis (GE). Samples from this case-control study were used to perform a detailed analysis on the presence of
D. fragilis
and
Blastocystis
in relation to gastrointestinal symptoms. In the present study, a real-time PCR for
Blastocystis
was performed on 1374 case samples and 1026 control samples from the multicenter gastroenteritis case-control study previously tested for
D. fragilis
. Prevalence of both micro-organisms was highest in children under 20 years of age and lowest in the oldest age group. A significantly lower overall detection of
D. fragilis
and
Blastocystis
was found in cases (both 25.8%) as compared to controls (37.6% and 40.0%, respectively). The difference for
D. fragilis
was statistically significant for subjects above 20 years of age. For
Blastocystis
, the difference was statistically significant in all age groups, except in children less than 5 years of age. A negative relation between
D. fragilis
-positive cases and diarrhea was found in this study population. More GE symptoms were reported in cases without
D. fragilis
or
Blastocystis
. In the present study, prevalence of both
D. fragilis
and
Blastocystis
is lower in cases with gastroenteritic symptoms than in controls. Besides, in cases with
D. fragilis
or
Blastocystis
, no association is shown between any of the GE symptoms. Interestingly, this suggests that the presence of these protozoans may be considered characteristic of a healthy intestinal microbiome.</description><identifier>ISSN: 0934-9723</identifier><identifier>EISSN: 1435-4373</identifier><identifier>DOI: 10.1007/s10096-019-03710-z</identifier><identifier>PMID: 31659566</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Age ; Biomedical and Life Sciences ; Biomedicine ; Blastocystis ; Children ; Diarrhea ; Dientamoeba fragilis ; Gastroenteritis ; Gastrointestinal symptoms ; Internal Medicine ; Intestine ; Medical Microbiology ; Microbiomes ; Original Article ; Population studies ; Samples ; Statistical analysis ; Statistical methods ; Statistical significance</subject><ispartof>European journal of clinical microbiology & infectious diseases, 2020, Vol.39 (1), p.197-203</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>European Journal of Clinical Microbiology & Infectious Diseases is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-d0dc910b54324f6f540576b50a836e2cb4aad93e7e28fbbb63011f79261768833</citedby><cites>FETCH-LOGICAL-c375t-d0dc910b54324f6f540576b50a836e2cb4aad93e7e28fbbb63011f79261768833</cites><orcidid>0000-0002-3985-1203</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31659566$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Boer, Maria Dullaert</creatorcontrib><creatorcontrib>Schuurs, Theo A.</creatorcontrib><creatorcontrib>Vermeer, Marloes</creatorcontrib><creatorcontrib>Ruijs, Gijs J.H.M.</creatorcontrib><creatorcontrib>van der Zanden, Adri G. M.</creatorcontrib><creatorcontrib>Weel, Jan F.</creatorcontrib><creatorcontrib>Bruijnesteijn van Coppenraet, Lesla E.S.</creatorcontrib><title>Distribution and relevance of Dientamoeba fragilis and Blastocystis species in gastroenteritis: results from a case-control study</title><title>European journal of clinical microbiology & infectious diseases</title><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><description>The actual role of
Dientamoeba fragilis
and
Blastocystis
in patients with gastrointestinal symptoms is still under debate. A multicenter case-control study was performed in The Netherlands to elucidate the clinical relevance of molecular diagnostics results in gastroenteritis (GE). Samples from this case-control study were used to perform a detailed analysis on the presence of
D. fragilis
and
Blastocystis
in relation to gastrointestinal symptoms. In the present study, a real-time PCR for
Blastocystis
was performed on 1374 case samples and 1026 control samples from the multicenter gastroenteritis case-control study previously tested for
D. fragilis
. Prevalence of both micro-organisms was highest in children under 20 years of age and lowest in the oldest age group. A significantly lower overall detection of
D. fragilis
and
Blastocystis
was found in cases (both 25.8%) as compared to controls (37.6% and 40.0%, respectively). The difference for
D. fragilis
was statistically significant for subjects above 20 years of age. For
Blastocystis
, the difference was statistically significant in all age groups, except in children less than 5 years of age. A negative relation between
D. fragilis
-positive cases and diarrhea was found in this study population. More GE symptoms were reported in cases without
D. fragilis
or
Blastocystis
. In the present study, prevalence of both
D. fragilis
and
Blastocystis
is lower in cases with gastroenteritic symptoms than in controls. Besides, in cases with
D. fragilis
or
Blastocystis
, no association is shown between any of the GE symptoms. Interestingly, this suggests that the presence of these protozoans may be considered characteristic of a healthy intestinal microbiome.</description><subject>Age</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blastocystis</subject><subject>Children</subject><subject>Diarrhea</subject><subject>Dientamoeba fragilis</subject><subject>Gastroenteritis</subject><subject>Gastrointestinal symptoms</subject><subject>Internal Medicine</subject><subject>Intestine</subject><subject>Medical Microbiology</subject><subject>Microbiomes</subject><subject>Original Article</subject><subject>Population studies</subject><subject>Samples</subject><subject>Statistical analysis</subject><subject>Statistical methods</subject><subject>Statistical significance</subject><issn>0934-9723</issn><issn>1435-4373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kU-LFDEQxYMo7rj6BTxIwIuX1qSrk3S86a7_YMGLnkOSrh6ydHfGVLcwe_ObG3dWBQ9eqqDq914VPMaeSvFSCmFeUa1WN0LaRoCRorm5x3ayA9V0YOA-2wkLXWNNC2fsEdG1qKLemIfsDKRWVmm9Yz8uE60lhW1NeeF-GXjBCb_7JSLPI79MuKx-zhg8H4vfpynRLfV28rTmeKS1DuiAMSHxtPB9HZdcRVhSXb2udrRNK1V1nrnn0RM2MS8Vmjit23B8zB6MfiJ8ctfP2df3775cfGyuPn_4dPHmqolg1NoMYohWiqA6aLtRj6oTyuighO9BYxtD5_1gAQ22_RhC0CCkHI1ttTS67wHO2YuT76HkbxvS6uZEEafJL5g3ci1I0fZWg63o83_Q67yVpX5XqQ5AyXq8Uu2JiiUTFRzdoaTZl6OTwv0KyJ0CcjUgdxuQu6miZ3fWW5hx-CP5nUgF4ARQXS17LH9v_8f2Jx1GnbM</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>de Boer, Maria Dullaert</creator><creator>Schuurs, Theo A.</creator><creator>Vermeer, Marloes</creator><creator>Ruijs, Gijs J.H.M.</creator><creator>van der Zanden, Adri G. M.</creator><creator>Weel, Jan F.</creator><creator>Bruijnesteijn van Coppenraet, Lesla E.S.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3985-1203</orcidid></search><sort><creationdate>2020</creationdate><title>Distribution and relevance of Dientamoeba fragilis and Blastocystis species in gastroenteritis: results from a case-control study</title><author>de Boer, Maria Dullaert ; Schuurs, Theo A. ; Vermeer, Marloes ; Ruijs, Gijs J.H.M. ; van der Zanden, Adri G. M. ; Weel, Jan F. ; Bruijnesteijn van Coppenraet, Lesla E.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-d0dc910b54324f6f540576b50a836e2cb4aad93e7e28fbbb63011f79261768833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Age</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blastocystis</topic><topic>Children</topic><topic>Diarrhea</topic><topic>Dientamoeba fragilis</topic><topic>Gastroenteritis</topic><topic>Gastrointestinal symptoms</topic><topic>Internal Medicine</topic><topic>Intestine</topic><topic>Medical Microbiology</topic><topic>Microbiomes</topic><topic>Original Article</topic><topic>Population studies</topic><topic>Samples</topic><topic>Statistical analysis</topic><topic>Statistical methods</topic><topic>Statistical significance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Boer, Maria Dullaert</creatorcontrib><creatorcontrib>Schuurs, Theo A.</creatorcontrib><creatorcontrib>Vermeer, Marloes</creatorcontrib><creatorcontrib>Ruijs, Gijs J.H.M.</creatorcontrib><creatorcontrib>van der Zanden, Adri G. 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M.</au><au>Weel, Jan F.</au><au>Bruijnesteijn van Coppenraet, Lesla E.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution and relevance of Dientamoeba fragilis and Blastocystis species in gastroenteritis: results from a case-control study</atitle><jtitle>European journal of clinical microbiology & infectious diseases</jtitle><stitle>Eur J Clin Microbiol Infect Dis</stitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><date>2020</date><risdate>2020</risdate><volume>39</volume><issue>1</issue><spage>197</spage><epage>203</epage><pages>197-203</pages><issn>0934-9723</issn><eissn>1435-4373</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>The actual role of
Dientamoeba fragilis
and
Blastocystis
in patients with gastrointestinal symptoms is still under debate. A multicenter case-control study was performed in The Netherlands to elucidate the clinical relevance of molecular diagnostics results in gastroenteritis (GE). Samples from this case-control study were used to perform a detailed analysis on the presence of
D. fragilis
and
Blastocystis
in relation to gastrointestinal symptoms. In the present study, a real-time PCR for
Blastocystis
was performed on 1374 case samples and 1026 control samples from the multicenter gastroenteritis case-control study previously tested for
D. fragilis
. Prevalence of both micro-organisms was highest in children under 20 years of age and lowest in the oldest age group. A significantly lower overall detection of
D. fragilis
and
Blastocystis
was found in cases (both 25.8%) as compared to controls (37.6% and 40.0%, respectively). The difference for
D. fragilis
was statistically significant for subjects above 20 years of age. For
Blastocystis
, the difference was statistically significant in all age groups, except in children less than 5 years of age. A negative relation between
D. fragilis
-positive cases and diarrhea was found in this study population. More GE symptoms were reported in cases without
D. fragilis
or
Blastocystis
. In the present study, prevalence of both
D. fragilis
and
Blastocystis
is lower in cases with gastroenteritic symptoms than in controls. Besides, in cases with
D. fragilis
or
Blastocystis
, no association is shown between any of the GE symptoms. Interestingly, this suggests that the presence of these protozoans may be considered characteristic of a healthy intestinal microbiome.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31659566</pmid><doi>10.1007/s10096-019-03710-z</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-3985-1203</orcidid></addata></record> |
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language | eng |
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subjects | Age Biomedical and Life Sciences Biomedicine Blastocystis Children Diarrhea Dientamoeba fragilis Gastroenteritis Gastrointestinal symptoms Internal Medicine Intestine Medical Microbiology Microbiomes Original Article Population studies Samples Statistical analysis Statistical methods Statistical significance |
title | Distribution and relevance of Dientamoeba fragilis and Blastocystis species in gastroenteritis: results from a case-control study |
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