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Constitutive expression of GmF6'H1 from soybean improves salt tolerance in transgenic Arabidopsis
Coumarin plays a pivotal role in plant response to biotic stress, as well as in the mediation of nutrient acquisition. However, its functions in response to abiotic stresses are largely unknown. In this work, a homologous gene, GmF6'H1, of AtF6'H1, which encodes the enzyme catalyzing the f...
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| Published in: | Plant physiology and biochemistry 2019-08, Vol.141, p.446-455 |
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| Main Authors: | , , , , , , , , , , , , , |
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| container_end_page | 455 |
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| container_start_page | 446 |
| container_title | Plant physiology and biochemistry |
| container_volume | 141 |
| creator | Duan, Chunli Mao, Tingting Sun, Shenqing Guo, Xianjun Guo, Laixian Huang, Lilong Wang, Zixuan Zhang, Yan Li, Miao Sheng, Yuting Yi, Yanjun Liu, Jiayao Zhang, Hongxia Zhang, Juan |
| description | Coumarin plays a pivotal role in plant response to biotic stress, as well as in the mediation of nutrient acquisition. However, its functions in response to abiotic stresses are largely unknown. In this work, a homologous gene, GmF6'H1, of AtF6'H1, which encodes the enzyme catalyzing the final rate-limiting step in the biosynthesis pathway of coumarin, was isolated from soybean. GmF6'H1 protein shares very high amino acid identity with AtF6'H1, and expression of GmF6'H1 in atf6'h1 can successfully restore the decreased coumarin production in the T-DNA insertion mutant. Further study revealed that the expression of GmF6'H1 in soybean was remarkably induced by salt stress. Constitutive expression of GmF6'H1 in Arabidopsis, driven by 35S promoter, significantly enhanced the resistance to salt of transgenic Arabidopsis. All these results suggest that GmF6'H1 can be used as a potential candidate gene for the engineering of plants with improved resistance to both biotic and abiotic stresses. |
| doi_str_mv | 10.1016/j.plaphy.2019.06.027 |
| format | article |
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However, its functions in response to abiotic stresses are largely unknown. In this work, a homologous gene, GmF6'H1, of AtF6'H1, which encodes the enzyme catalyzing the final rate-limiting step in the biosynthesis pathway of coumarin, was isolated from soybean. GmF6'H1 protein shares very high amino acid identity with AtF6'H1, and expression of GmF6'H1 in atf6'h1 can successfully restore the decreased coumarin production in the T-DNA insertion mutant. Further study revealed that the expression of GmF6'H1 in soybean was remarkably induced by salt stress. Constitutive expression of GmF6'H1 in Arabidopsis, driven by 35S promoter, significantly enhanced the resistance to salt of transgenic Arabidopsis. All these results suggest that GmF6'H1 can be used as a potential candidate gene for the engineering of plants with improved resistance to both biotic and abiotic stresses.</description><identifier>EISSN: 1873-2690</identifier><identifier>DOI: 10.1016/j.plaphy.2019.06.027</identifier><identifier>PMID: 31247427</identifier><language>eng</language><publisher>France</publisher><subject>Arabidopsis - genetics ; Arabidopsis - physiology ; Chlorophyll - chemistry ; Cloning, Molecular ; Coumarins - chemistry ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Germination ; Glycine max - enzymology ; Glycine max - genetics ; Phenotype ; Plant Leaves - metabolism ; Plants, Genetically Modified - physiology ; Promoter Regions, Genetic ; Salt Tolerance</subject><ispartof>Plant physiology and biochemistry, 2019-08, Vol.141, p.446-455</ispartof><rights>Copyright © 2019. Published by Elsevier Masson SAS.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31247427$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duan, Chunli</creatorcontrib><creatorcontrib>Mao, Tingting</creatorcontrib><creatorcontrib>Sun, Shenqing</creatorcontrib><creatorcontrib>Guo, Xianjun</creatorcontrib><creatorcontrib>Guo, Laixian</creatorcontrib><creatorcontrib>Huang, Lilong</creatorcontrib><creatorcontrib>Wang, Zixuan</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Li, Miao</creatorcontrib><creatorcontrib>Sheng, Yuting</creatorcontrib><creatorcontrib>Yi, Yanjun</creatorcontrib><creatorcontrib>Liu, Jiayao</creatorcontrib><creatorcontrib>Zhang, Hongxia</creatorcontrib><creatorcontrib>Zhang, Juan</creatorcontrib><title>Constitutive expression of GmF6'H1 from soybean improves salt tolerance in transgenic Arabidopsis</title><title>Plant physiology and biochemistry</title><addtitle>Plant Physiol Biochem</addtitle><description>Coumarin plays a pivotal role in plant response to biotic stress, as well as in the mediation of nutrient acquisition. However, its functions in response to abiotic stresses are largely unknown. In this work, a homologous gene, GmF6'H1, of AtF6'H1, which encodes the enzyme catalyzing the final rate-limiting step in the biosynthesis pathway of coumarin, was isolated from soybean. GmF6'H1 protein shares very high amino acid identity with AtF6'H1, and expression of GmF6'H1 in atf6'h1 can successfully restore the decreased coumarin production in the T-DNA insertion mutant. Further study revealed that the expression of GmF6'H1 in soybean was remarkably induced by salt stress. Constitutive expression of GmF6'H1 in Arabidopsis, driven by 35S promoter, significantly enhanced the resistance to salt of transgenic Arabidopsis. All these results suggest that GmF6'H1 can be used as a potential candidate gene for the engineering of plants with improved resistance to both biotic and abiotic stresses.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - physiology</subject><subject>Chlorophyll - chemistry</subject><subject>Cloning, Molecular</subject><subject>Coumarins - chemistry</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant</subject><subject>Germination</subject><subject>Glycine max - enzymology</subject><subject>Glycine max - genetics</subject><subject>Phenotype</subject><subject>Plant Leaves - metabolism</subject><subject>Plants, Genetically Modified - physiology</subject><subject>Promoter Regions, Genetic</subject><subject>Salt Tolerance</subject><issn>1873-2690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNo1kE1Lw0AURQdBbK3-A5HZ6SZxvjKTLEuxrVBw032YSV51SjIT85Ji_72B1tU9i8Plcgl54izljOu3Y9o1tvs-p4LxImU6ZcLckDnPjUyELtiM3CMeGWNCGXlHZpJPoISZE7uKAQc_jIM_AYXfrgdEHwONB7pp1_ply-mhjy3FeHZgA_Vt18cTIEXbDHSIDfQ2VEB9oMNE-AXBV3TZW-fr2KHHB3J7sA3C4zUXZL9-36-2ye5z87Fa7pIu0ybJRAF5BtY4bqq6KEA7J7lWSoN1xmruKm5AOiU0mDwrQGVVLZ1kTru8VpVckNdL7TTvZwQcytZjBU1jA8QRSyEypoXguZzU56s6uhbqsut9a_tz-f-K_AOgl2Vz</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Duan, Chunli</creator><creator>Mao, Tingting</creator><creator>Sun, Shenqing</creator><creator>Guo, Xianjun</creator><creator>Guo, Laixian</creator><creator>Huang, Lilong</creator><creator>Wang, Zixuan</creator><creator>Zhang, Yan</creator><creator>Li, Miao</creator><creator>Sheng, Yuting</creator><creator>Yi, Yanjun</creator><creator>Liu, Jiayao</creator><creator>Zhang, Hongxia</creator><creator>Zhang, Juan</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201908</creationdate><title>Constitutive expression of GmF6'H1 from soybean improves salt tolerance in transgenic Arabidopsis</title><author>Duan, Chunli ; Mao, Tingting ; Sun, Shenqing ; Guo, Xianjun ; Guo, Laixian ; Huang, Lilong ; Wang, Zixuan ; Zhang, Yan ; Li, Miao ; Sheng, Yuting ; Yi, Yanjun ; Liu, Jiayao ; Zhang, Hongxia ; Zhang, Juan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p567-529e85ea7b17cd99e6bb316446eab7a61bc17e3b426e7859e45cd3b30b6b8d4c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - physiology</topic><topic>Chlorophyll - chemistry</topic><topic>Cloning, Molecular</topic><topic>Coumarins - chemistry</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant</topic><topic>Germination</topic><topic>Glycine max - enzymology</topic><topic>Glycine max - genetics</topic><topic>Phenotype</topic><topic>Plant Leaves - metabolism</topic><topic>Plants, Genetically Modified - physiology</topic><topic>Promoter Regions, Genetic</topic><topic>Salt Tolerance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duan, Chunli</creatorcontrib><creatorcontrib>Mao, Tingting</creatorcontrib><creatorcontrib>Sun, Shenqing</creatorcontrib><creatorcontrib>Guo, Xianjun</creatorcontrib><creatorcontrib>Guo, Laixian</creatorcontrib><creatorcontrib>Huang, Lilong</creatorcontrib><creatorcontrib>Wang, Zixuan</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Li, Miao</creatorcontrib><creatorcontrib>Sheng, Yuting</creatorcontrib><creatorcontrib>Yi, Yanjun</creatorcontrib><creatorcontrib>Liu, Jiayao</creatorcontrib><creatorcontrib>Zhang, Hongxia</creatorcontrib><creatorcontrib>Zhang, Juan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duan, Chunli</au><au>Mao, Tingting</au><au>Sun, Shenqing</au><au>Guo, Xianjun</au><au>Guo, Laixian</au><au>Huang, Lilong</au><au>Wang, Zixuan</au><au>Zhang, Yan</au><au>Li, Miao</au><au>Sheng, Yuting</au><au>Yi, Yanjun</au><au>Liu, Jiayao</au><au>Zhang, Hongxia</au><au>Zhang, Juan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Constitutive expression of GmF6'H1 from soybean improves salt tolerance in transgenic Arabidopsis</atitle><jtitle>Plant physiology and biochemistry</jtitle><addtitle>Plant Physiol Biochem</addtitle><date>2019-08</date><risdate>2019</risdate><volume>141</volume><spage>446</spage><epage>455</epage><pages>446-455</pages><eissn>1873-2690</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Coumarin plays a pivotal role in plant response to biotic stress, as well as in the mediation of nutrient acquisition. 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All these results suggest that GmF6'H1 can be used as a potential candidate gene for the engineering of plants with improved resistance to both biotic and abiotic stresses.</abstract><cop>France</cop><pmid>31247427</pmid><doi>10.1016/j.plaphy.2019.06.027</doi><tpages>10</tpages></addata></record> |
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| ispartof | Plant physiology and biochemistry, 2019-08, Vol.141, p.446-455 |
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| subjects | Arabidopsis - genetics Arabidopsis - physiology Chlorophyll - chemistry Cloning, Molecular Coumarins - chemistry Gene Expression Profiling Gene Expression Regulation, Plant Germination Glycine max - enzymology Glycine max - genetics Phenotype Plant Leaves - metabolism Plants, Genetically Modified - physiology Promoter Regions, Genetic Salt Tolerance |
| title | Constitutive expression of GmF6'H1 from soybean improves salt tolerance in transgenic Arabidopsis |
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