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Biochemical and molecular biomarkers in integument biopsies of free-ranging coastal bottlenose dolphins from southern Brazil
Adverse effects of exposure to persistent organic pollutants (POPs) threaten the maintenance of odontocete populations. In southern Brazil, coastal bottlenose dolphins from the Laguna Estuarine System (LES) and Patos Lagoon Estuary (PLE) were sampled using remote biopsies during the winter and summe...
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| Published in: | Chemosphere (Oxford) 2019-06, Vol.225, p.139-149 |
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| creator | Righetti, Barbara Pacheco Harrison Mattos, Jacó Joaquim Siebert, Marília Nardelli Daura-Jorge, Fábio Gonçalves Bezamat, Carolina Fruet, Pedro Friedrich Genoves, Rodrigo Cezar Taniguchi, Satie da Silva, Josilene Montone, Rosalinda Carmela Simões-Lopes, Paulo César de Azevedo Bainy, Afonso Celso Dias Lüchmann, Karim Hahn |
| description | Adverse effects of exposure to persistent organic pollutants (POPs) threaten the maintenance of odontocete populations. In southern Brazil, coastal bottlenose dolphins from the Laguna Estuarine System (LES) and Patos Lagoon Estuary (PLE) were sampled using remote biopsies during the winter and summer months. Levels of bioaccumulated POPs were measured in the blubber. The activities of glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx), and superoxide dismutase (SOD) were also quantified, as were the mRNA transcript levels of aryl hydrocarbon receptor (AhR), AhR nuclear translocator (ARNT), cytochrome P450 1A1-like (CYP1A1), metallothionein 2A (MT2A), GST-π, GPx-4, GR, interleukin 1 alpha (IL-1α), and major histocompatibility complex II (MHCII) in the skin. In general, levels of POPs were similar among sites, sexes, ages and seasons. For most animals, total polychlorinated biphenyl (ΣPCBs) levels were above the threshold level have physiological effects and pose risks to cetaceans. The best-fitting generalized linear models (GLMs) found significant associations between GR, IL-1α and GPx-4 transcript levels, SOD and GST activities, and total polybrominated diphenyl ether (ΣPBDEs) and pesticide levels. GLMs and Kruskal-Wallis analyses also indicated that there were higher transcript levels for most genes and lower GST activity in the winter. These results reinforce the need to consider the influence of environmental traits on biomarker values in wildlife assessments.
•PCB levels were above the threshold established as risk limit for Tursiops truncatus.•Transcript levels of GR, GPx-4 and IL-1α were associated with PBDEs and pesticides.•Higher GST activity was associated with higher pesticides levels.•Data suggests a putative influence of seasonality on biomarker responses. |
| doi_str_mv | 10.1016/j.chemosphere.2019.02.179 |
| format | article |
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In southern Brazil, coastal bottlenose dolphins from the Laguna Estuarine System (LES) and Patos Lagoon Estuary (PLE) were sampled using remote biopsies during the winter and summer months. Levels of bioaccumulated POPs were measured in the blubber. The activities of glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx), and superoxide dismutase (SOD) were also quantified, as were the mRNA transcript levels of aryl hydrocarbon receptor (AhR), AhR nuclear translocator (ARNT), cytochrome P450 1A1-like (CYP1A1), metallothionein 2A (MT2A), GST-π, GPx-4, GR, interleukin 1 alpha (IL-1α), and major histocompatibility complex II (MHCII) in the skin. In general, levels of POPs were similar among sites, sexes, ages and seasons. For most animals, total polychlorinated biphenyl (ΣPCBs) levels were above the threshold level have physiological effects and pose risks to cetaceans. The best-fitting generalized linear models (GLMs) found significant associations between GR, IL-1α and GPx-4 transcript levels, SOD and GST activities, and total polybrominated diphenyl ether (ΣPBDEs) and pesticide levels. GLMs and Kruskal-Wallis analyses also indicated that there were higher transcript levels for most genes and lower GST activity in the winter. These results reinforce the need to consider the influence of environmental traits on biomarker values in wildlife assessments.
•PCB levels were above the threshold established as risk limit for Tursiops truncatus.•Transcript levels of GR, GPx-4 and IL-1α were associated with PBDEs and pesticides.•Higher GST activity was associated with higher pesticides levels.•Data suggests a putative influence of seasonality on biomarker responses.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>30870631</pmid><doi>10.1016/j.chemosphere.2019.02.179</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biomarker response Bottlenose dolphins Organic contaminants |
| title | Biochemical and molecular biomarkers in integument biopsies of free-ranging coastal bottlenose dolphins from southern Brazil |
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