Loading…
Prevalence and Impact of Bacteriophages on the Presence of Escherichia coli O157:H7 in Feedlot Cattle and Their Environment
The relationship between endemic bacteriophages infecting E. coli O157:H7 (referred to as "phage") and levels of shedding of E. coli O157:H7 by cattle was investigated in two commercial feedlots in southern Alberta, Canada. Between May and November 2007, 10 pens of cattle were monitored by...
Saved in:
| Published in: | Applied and Environmental Microbiology 2009-03, Vol.75 (5), p.1271-1278 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c520t-5593725692a76599ca5fc1d2b4cb7b4ed75a376c7d0a9a479b1222aa49099bf73 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c520t-5593725692a76599ca5fc1d2b4cb7b4ed75a376c7d0a9a479b1222aa49099bf73 |
| container_end_page | 1278 |
| container_issue | 5 |
| container_start_page | 1271 |
| container_title | Applied and Environmental Microbiology |
| container_volume | 75 |
| creator | Niu, Y.D McAllister, T.A Xu, Y Johnson, R.P Stephens, T.P Stanford, K |
| description | The relationship between endemic bacteriophages infecting E. coli O157:H7 (referred to as "phage") and levels of shedding of E. coli O157:H7 by cattle was investigated in two commercial feedlots in southern Alberta, Canada. Between May and November 2007, 10 pens of cattle were monitored by collection of pooled fecal pats, water with sediment from troughs, manure slurry from the pen floor, and rectal fecal samples from individual animals (20 per pen) at two separate times. Bacteriophages infecting E. coli O157:H7 were detected more frequently (P < 0.001) after 18 to 20 h enrichment than by initial screening and were recovered in 239 of 855 samples (26.5% of 411 pooled fecal pats, 23.8% of 320 fecal grab samples, 21.8% of 87 water trough samples, and 94.6% of 37 pen floor slurry samples). Overall, prevalence of phage was highest (P < 0.001) in slurry. Recovery of phage from pooled fecal pats was highest (P < 0.05) in May. Overall recovery did not differ (P > 0.10) between fecal grab samples and pooled fecal pats. A higher prevalence of phage in fecal pats or water trough samples was associated (P < 0.01) with reduced prevalence of E. coli O157:H7 in rectal fecal samples. There was a weak but significant negative correlation between isolation of phage and E. coli O157:H7 in fecal grab samples (r = -0.11; P < 0.05). These data demonstrate that the prevalence of phage fluctuates in a manner similar to that described for E. coli O157:H7. Phage were more prevalent in manure slurry than other environmental sources. The likelihood of fecal shedding of E. coli O157:H7 was reduced if cattle in the pen harbored phage. |
| doi_str_mv | 10.1128/AEM.02100-08 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_21500448</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1653047871</sourcerecordid><originalsourceid>FETCH-LOGICAL-c520t-5593725692a76599ca5fc1d2b4cb7b4ed75a376c7d0a9a479b1222aa49099bf73</originalsourceid><addsrcrecordid>eNqF0Utv1DAUBeAIgWgp7FiDQYIVU64dP2IWSGU0pZWKikS7tm4cZ-Iqiad2ZhDiz-PpjMpjw8oLfzrX16conlM4ppRV708WX46BUYAZVA-KQwq6momylA-LQwCtZ4xxOCiepHQDABxk9bg4oJqWmvHysPj5NboN9m60juDYkPNhhXYioSWf8umiD6sOly6RMJKpcyTzdIezWCTbZWE7j8SG3pNLKtSHM0X8SE6da_owkTlOU7-Lvuqcj2QxbnwM4-DG6WnxqMU-uWf786i4Pl1czc9mF5efz-cnFzMrGEwzIXSpmJCaoZJCa4uitbRhNbe1qrlrlMBSSasaQI1c6ZoyxhC5zuvXrSqPio-73NW6Hlxj8-iIvVlFP2D8YQJ68_fN6DuzDBvDJK-oLHPA231ADLdrlyYz-GRd3-PowjoZKbWQCuC_kFGRO-BVhq__gTdhHcf8C4aB0FIxzjN6t0M2hpSia--fTMFsuze5e3PXvYFt5os_1_yN92Vn8GYPMFns24ij9eneMcogz90Gvdq5zi-77z46g2kw6AajhBGGMkWzebkzLQaDy5hzrr8xoCVQoSslefkLcnjJ6A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>205967244</pqid></control><display><type>article</type><title>Prevalence and Impact of Bacteriophages on the Presence of Escherichia coli O157:H7 in Feedlot Cattle and Their Environment</title><source>American Society for Microbiology</source><source>PubMed Central</source><creator>Niu, Y.D ; McAllister, T.A ; Xu, Y ; Johnson, R.P ; Stephens, T.P ; Stanford, K</creator><creatorcontrib>Niu, Y.D ; McAllister, T.A ; Xu, Y ; Johnson, R.P ; Stephens, T.P ; Stanford, K</creatorcontrib><description>The relationship between endemic bacteriophages infecting E. coli O157:H7 (referred to as "phage") and levels of shedding of E. coli O157:H7 by cattle was investigated in two commercial feedlots in southern Alberta, Canada. Between May and November 2007, 10 pens of cattle were monitored by collection of pooled fecal pats, water with sediment from troughs, manure slurry from the pen floor, and rectal fecal samples from individual animals (20 per pen) at two separate times. Bacteriophages infecting E. coli O157:H7 were detected more frequently (P < 0.001) after 18 to 20 h enrichment than by initial screening and were recovered in 239 of 855 samples (26.5% of 411 pooled fecal pats, 23.8% of 320 fecal grab samples, 21.8% of 87 water trough samples, and 94.6% of 37 pen floor slurry samples). Overall, prevalence of phage was highest (P < 0.001) in slurry. Recovery of phage from pooled fecal pats was highest (P < 0.05) in May. Overall recovery did not differ (P > 0.10) between fecal grab samples and pooled fecal pats. A higher prevalence of phage in fecal pats or water trough samples was associated (P < 0.01) with reduced prevalence of E. coli O157:H7 in rectal fecal samples. There was a weak but significant negative correlation between isolation of phage and E. coli O157:H7 in fecal grab samples (r = -0.11; P < 0.05). These data demonstrate that the prevalence of phage fluctuates in a manner similar to that described for E. coli O157:H7. Phage were more prevalent in manure slurry than other environmental sources. The likelihood of fecal shedding of E. coli O157:H7 was reduced if cattle in the pen harbored phage.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AEM.02100-08</identifier><identifier>PMID: 19139243</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Alberta ; Animals ; Biological and medical sciences ; Cattle ; Coliphages - isolation & purification ; Colony Count, Microbial ; Data processing ; E coli ; Environmental science ; Escherichia coli ; Escherichia coli O157 - isolation & purification ; Escherichia coli O157 - virology ; Factory farming ; Feces - microbiology ; Feces - virology ; Fundamental and applied biological sciences. Psychology ; Livestock industry ; Manures ; Microbiology ; Public Health Microbiology ; Seasons ; Sewage - microbiology ; Sewage - virology</subject><ispartof>Applied and Environmental Microbiology, 2009-03, Vol.75 (5), p.1271-1278</ispartof><rights>2009 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Mar 2009</rights><rights>Copyright © 2009, American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520t-5593725692a76599ca5fc1d2b4cb7b4ed75a376c7d0a9a479b1222aa49099bf73</citedby><cites>FETCH-LOGICAL-c520t-5593725692a76599ca5fc1d2b4cb7b4ed75a376c7d0a9a479b1222aa49099bf73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2648163/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2648163/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,733,786,790,891,3207,3208,27957,27958,53827,53829</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21207248$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19139243$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Niu, Y.D</creatorcontrib><creatorcontrib>McAllister, T.A</creatorcontrib><creatorcontrib>Xu, Y</creatorcontrib><creatorcontrib>Johnson, R.P</creatorcontrib><creatorcontrib>Stephens, T.P</creatorcontrib><creatorcontrib>Stanford, K</creatorcontrib><title>Prevalence and Impact of Bacteriophages on the Presence of Escherichia coli O157:H7 in Feedlot Cattle and Their Environment</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>The relationship between endemic bacteriophages infecting E. coli O157:H7 (referred to as "phage") and levels of shedding of E. coli O157:H7 by cattle was investigated in two commercial feedlots in southern Alberta, Canada. Between May and November 2007, 10 pens of cattle were monitored by collection of pooled fecal pats, water with sediment from troughs, manure slurry from the pen floor, and rectal fecal samples from individual animals (20 per pen) at two separate times. Bacteriophages infecting E. coli O157:H7 were detected more frequently (P < 0.001) after 18 to 20 h enrichment than by initial screening and were recovered in 239 of 855 samples (26.5% of 411 pooled fecal pats, 23.8% of 320 fecal grab samples, 21.8% of 87 water trough samples, and 94.6% of 37 pen floor slurry samples). Overall, prevalence of phage was highest (P < 0.001) in slurry. Recovery of phage from pooled fecal pats was highest (P < 0.05) in May. Overall recovery did not differ (P > 0.10) between fecal grab samples and pooled fecal pats. A higher prevalence of phage in fecal pats or water trough samples was associated (P < 0.01) with reduced prevalence of E. coli O157:H7 in rectal fecal samples. There was a weak but significant negative correlation between isolation of phage and E. coli O157:H7 in fecal grab samples (r = -0.11; P < 0.05). These data demonstrate that the prevalence of phage fluctuates in a manner similar to that described for E. coli O157:H7. Phage were more prevalent in manure slurry than other environmental sources. The likelihood of fecal shedding of E. coli O157:H7 was reduced if cattle in the pen harbored phage.</description><subject>Alberta</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Coliphages - isolation & purification</subject><subject>Colony Count, Microbial</subject><subject>Data processing</subject><subject>E coli</subject><subject>Environmental science</subject><subject>Escherichia coli</subject><subject>Escherichia coli O157 - isolation & purification</subject><subject>Escherichia coli O157 - virology</subject><subject>Factory farming</subject><subject>Feces - microbiology</subject><subject>Feces - virology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Livestock industry</subject><subject>Manures</subject><subject>Microbiology</subject><subject>Public Health Microbiology</subject><subject>Seasons</subject><subject>Sewage - microbiology</subject><subject>Sewage - virology</subject><issn>0099-2240</issn><issn>1098-5336</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqF0Utv1DAUBeAIgWgp7FiDQYIVU64dP2IWSGU0pZWKikS7tm4cZ-Iqiad2ZhDiz-PpjMpjw8oLfzrX16conlM4ppRV708WX46BUYAZVA-KQwq6momylA-LQwCtZ4xxOCiepHQDABxk9bg4oJqWmvHysPj5NboN9m60juDYkPNhhXYioSWf8umiD6sOly6RMJKpcyTzdIezWCTbZWE7j8SG3pNLKtSHM0X8SE6da_owkTlOU7-Lvuqcj2QxbnwM4-DG6WnxqMU-uWf786i4Pl1czc9mF5efz-cnFzMrGEwzIXSpmJCaoZJCa4uitbRhNbe1qrlrlMBSSasaQI1c6ZoyxhC5zuvXrSqPio-73NW6Hlxj8-iIvVlFP2D8YQJ68_fN6DuzDBvDJK-oLHPA231ADLdrlyYz-GRd3-PowjoZKbWQCuC_kFGRO-BVhq__gTdhHcf8C4aB0FIxzjN6t0M2hpSia--fTMFsuze5e3PXvYFt5os_1_yN92Vn8GYPMFns24ij9eneMcogz90Gvdq5zi-77z46g2kw6AajhBGGMkWzebkzLQaDy5hzrr8xoCVQoSslefkLcnjJ6A</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Niu, Y.D</creator><creator>McAllister, T.A</creator><creator>Xu, Y</creator><creator>Johnson, R.P</creator><creator>Stephens, T.P</creator><creator>Stanford, K</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090301</creationdate><title>Prevalence and Impact of Bacteriophages on the Presence of Escherichia coli O157:H7 in Feedlot Cattle and Their Environment</title><author>Niu, Y.D ; McAllister, T.A ; Xu, Y ; Johnson, R.P ; Stephens, T.P ; Stanford, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c520t-5593725692a76599ca5fc1d2b4cb7b4ed75a376c7d0a9a479b1222aa49099bf73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Alberta</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Coliphages - isolation & purification</topic><topic>Colony Count, Microbial</topic><topic>Data processing</topic><topic>E coli</topic><topic>Environmental science</topic><topic>Escherichia coli</topic><topic>Escherichia coli O157 - isolation & purification</topic><topic>Escherichia coli O157 - virology</topic><topic>Factory farming</topic><topic>Feces - microbiology</topic><topic>Feces - virology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Livestock industry</topic><topic>Manures</topic><topic>Microbiology</topic><topic>Public Health Microbiology</topic><topic>Seasons</topic><topic>Sewage - microbiology</topic><topic>Sewage - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Niu, Y.D</creatorcontrib><creatorcontrib>McAllister, T.A</creatorcontrib><creatorcontrib>Xu, Y</creatorcontrib><creatorcontrib>Johnson, R.P</creatorcontrib><creatorcontrib>Stephens, T.P</creatorcontrib><creatorcontrib>Stanford, K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Niu, Y.D</au><au>McAllister, T.A</au><au>Xu, Y</au><au>Johnson, R.P</au><au>Stephens, T.P</au><au>Stanford, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence and Impact of Bacteriophages on the Presence of Escherichia coli O157:H7 in Feedlot Cattle and Their Environment</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2009-03-01</date><risdate>2009</risdate><volume>75</volume><issue>5</issue><spage>1271</spage><epage>1278</epage><pages>1271-1278</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><eissn>1098-6596</eissn><coden>AEMIDF</coden><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><notes>ObjectType-Article-1</notes><notes>ObjectType-Feature-2</notes><notes>Corresponding author. Mailing address: Agriculture and Agri-Food Canada, Lethbridge Research Centre, P.O. Box 3000, Lethbridge, Alberta, Canada T1J 4B1. Phone: (403) 317-2240. Fax: (403) 382-3156. E-mail: mcallistert@agr.gc.ca</notes><abstract>The relationship between endemic bacteriophages infecting E. coli O157:H7 (referred to as "phage") and levels of shedding of E. coli O157:H7 by cattle was investigated in two commercial feedlots in southern Alberta, Canada. Between May and November 2007, 10 pens of cattle were monitored by collection of pooled fecal pats, water with sediment from troughs, manure slurry from the pen floor, and rectal fecal samples from individual animals (20 per pen) at two separate times. Bacteriophages infecting E. coli O157:H7 were detected more frequently (P < 0.001) after 18 to 20 h enrichment than by initial screening and were recovered in 239 of 855 samples (26.5% of 411 pooled fecal pats, 23.8% of 320 fecal grab samples, 21.8% of 87 water trough samples, and 94.6% of 37 pen floor slurry samples). Overall, prevalence of phage was highest (P < 0.001) in slurry. Recovery of phage from pooled fecal pats was highest (P < 0.05) in May. Overall recovery did not differ (P > 0.10) between fecal grab samples and pooled fecal pats. A higher prevalence of phage in fecal pats or water trough samples was associated (P < 0.01) with reduced prevalence of E. coli O157:H7 in rectal fecal samples. There was a weak but significant negative correlation between isolation of phage and E. coli O157:H7 in fecal grab samples (r = -0.11; P < 0.05). These data demonstrate that the prevalence of phage fluctuates in a manner similar to that described for E. coli O157:H7. Phage were more prevalent in manure slurry than other environmental sources. The likelihood of fecal shedding of E. coli O157:H7 was reduced if cattle in the pen harbored phage.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>19139243</pmid><doi>10.1128/AEM.02100-08</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0099-2240 |
| ispartof | Applied and Environmental Microbiology, 2009-03, Vol.75 (5), p.1271-1278 |
| issn | 0099-2240 1098-5336 1098-6596 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_21500448 |
| source | American Society for Microbiology; PubMed Central |
| subjects | Alberta Animals Biological and medical sciences Cattle Coliphages - isolation & purification Colony Count, Microbial Data processing E coli Environmental science Escherichia coli Escherichia coli O157 - isolation & purification Escherichia coli O157 - virology Factory farming Feces - microbiology Feces - virology Fundamental and applied biological sciences. Psychology Livestock industry Manures Microbiology Public Health Microbiology Seasons Sewage - microbiology Sewage - virology |
| title | Prevalence and Impact of Bacteriophages on the Presence of Escherichia coli O157:H7 in Feedlot Cattle and Their Environment |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T14%3A57%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Prevalence%20and%20Impact%20of%20Bacteriophages%20on%20the%20Presence%20of%20Escherichia%20coli%20O157:H7%20in%20Feedlot%20Cattle%20and%20Their%20Environment&rft.jtitle=Applied%20and%20Environmental%20Microbiology&rft.au=Niu,%20Y.D&rft.date=2009-03-01&rft.volume=75&rft.issue=5&rft.spage=1271&rft.epage=1278&rft.pages=1271-1278&rft.issn=0099-2240&rft.eissn=1098-5336&rft.coden=AEMIDF&rft_id=info:doi/10.1128/AEM.02100-08&rft_dat=%3Cproquest_cross%3E1653047871%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c520t-5593725692a76599ca5fc1d2b4cb7b4ed75a376c7d0a9a479b1222aa49099bf73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=205967244&rft_id=info:pmid/19139243&rfr_iscdi=true |