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Mechanically stimulated osteocytes reduce the bone‐metastatic potential of breast cancer cells in vitro by signaling through endothelial cells
Bone metastases occur in 65% to 75% of patients with advanced breast cancer and significantly worsen their survival and quality of life. We previously showed that conditioned medium (CM) from osteocytes stimulated with oscillatory fluid flow, mimicking bone mechanical loading during routine physical...
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| Published in: | Journal of cellular biochemistry 2019-05, Vol.120 (5), p.7590-7601 |
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| creator | Ma, Yu‐Heng Vivian Xu, Liangcheng Mei, Xueting Middleton, Kevin You, Lidan |
| description | Bone metastases occur in 65% to 75% of patients with advanced breast cancer and significantly worsen their survival and quality of life. We previously showed that conditioned medium (CM) from osteocytes stimulated with oscillatory fluid flow, mimicking bone mechanical loading during routine physical activities, reduced the transendothelial migration of breast cancer cells. Endothelial cells are situated at an ideal location to mediate signals between osteocytes in the bone matrix and metastasizing cancer cells in the blood vessels. In this study, we investigated the specific effects of flow‐stimulated osteocytes on the interaction between endothelial cells and breast cancer cells in vitro. We observed that CM from flow‐stimulated osteocytes reduced endothelial permeability by 15% and breast cancer cell adhesion onto endothelial monolayers by 18%. The difference in adhesion was abolished with anti‐intercellular adhesion molecule 1 (ICAM‐1) neutralizing antibodies. Furthermore, CM from endothelial cells conditioned in CM from flow‐stimulated osteocytes significantly altered the gene expression in bone‐metastatic breast cancer cells, as shown by RNA sequencing. Specifically, breast cancer cell expression of matrix metallopeptidase 9 (MMP‐9) was downregulated by 62%, and frizzled‐4 (FZD4) by 61%, when the osteocytes were stimulated with flow. The invasion of these breast cancer cells across Matrigel was also reduced by 47%, and this difference was abolished by MMP‐9 inhibitors. In conclusion, we demonstrated that flow‐stimulated osteocytes downregulate the bone‐metastatic potential of breast cancer cells by signaling through endothelial cells. This provides insights into the capability of bone mechanical regulation in preventing bone metastases; and may assist in prescribing exercise or bone‐loading regimens to patients with breast cancers.
This article investigates the effects of mechanically stimulated osteocytes on the interaction between endothelial cells and breast cancer cells to reduce the bone‐metastatic potential. The application of conditioned medium (CM) from flow‐stimulated osteocytes to endothelial cells reduces their permeability and adhesion by breast cancer cells. The application of CM from these conditioned endothelial cells to bone‐metastatic breast cancer cells reduces their matrix metallopeptidase 9 and FZD4 expression and invasiveness. |
| doi_str_mv | 10.1002/jcb.28034 |
| format | article |
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This article investigates the effects of mechanically stimulated osteocytes on the interaction between endothelial cells and breast cancer cells to reduce the bone‐metastatic potential. The application of conditioned medium (CM) from flow‐stimulated osteocytes to endothelial cells reduces their permeability and adhesion by breast cancer cells. The application of CM from these conditioned endothelial cells to bone‐metastatic breast cancer cells reduces their matrix metallopeptidase 9 and FZD4 expression and invasiveness.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.28034</identifier><identifier>PMID: 30417549</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Adhesion ; Antibodies ; Biocompatibility ; Biomedical materials ; Blood vessels ; Bone cancer ; Bone matrix ; bone metastasis ; Breast cancer ; Cancer ; Cell adhesion ; Cell adhesion & migration ; Cell migration ; Conditioning ; Endothelial cells ; Fluid dynamics ; Fluid flow ; Frizzled protein ; Gene expression ; Gene sequencing ; Intercellular adhesion molecule 1 ; invasion ; matrix metallopeptidase 9 (MMP‐9) ; Mechanical loading ; Metalloproteinase ; Metastases ; Metastasis ; Mimicry ; osteocyte ; Osteocytes ; Patients ; Permeability ; Quality of life ; Ribonucleic acid ; RNA ; RNA sequencing ; Signaling</subject><ispartof>Journal of cellular biochemistry, 2019-05, Vol.120 (5), p.7590-7601</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><rights>2019 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3534-b43421854a064498c8071dbd142f0e3290312d83f6fc6d5ef42602471e274ae33</citedby><cites>FETCH-LOGICAL-c3534-b43421854a064498c8071dbd142f0e3290312d83f6fc6d5ef42602471e274ae33</cites><orcidid>0000-0002-5884-114X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcb.28034$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcb.28034$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30417549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Yu‐Heng Vivian</creatorcontrib><creatorcontrib>Xu, Liangcheng</creatorcontrib><creatorcontrib>Mei, Xueting</creatorcontrib><creatorcontrib>Middleton, Kevin</creatorcontrib><creatorcontrib>You, Lidan</creatorcontrib><title>Mechanically stimulated osteocytes reduce the bone‐metastatic potential of breast cancer cells in vitro by signaling through endothelial cells</title><title>Journal of cellular biochemistry</title><addtitle>J Cell Biochem</addtitle><description>Bone metastases occur in 65% to 75% of patients with advanced breast cancer and significantly worsen their survival and quality of life. We previously showed that conditioned medium (CM) from osteocytes stimulated with oscillatory fluid flow, mimicking bone mechanical loading during routine physical activities, reduced the transendothelial migration of breast cancer cells. Endothelial cells are situated at an ideal location to mediate signals between osteocytes in the bone matrix and metastasizing cancer cells in the blood vessels. In this study, we investigated the specific effects of flow‐stimulated osteocytes on the interaction between endothelial cells and breast cancer cells in vitro. We observed that CM from flow‐stimulated osteocytes reduced endothelial permeability by 15% and breast cancer cell adhesion onto endothelial monolayers by 18%. The difference in adhesion was abolished with anti‐intercellular adhesion molecule 1 (ICAM‐1) neutralizing antibodies. Furthermore, CM from endothelial cells conditioned in CM from flow‐stimulated osteocytes significantly altered the gene expression in bone‐metastatic breast cancer cells, as shown by RNA sequencing. Specifically, breast cancer cell expression of matrix metallopeptidase 9 (MMP‐9) was downregulated by 62%, and frizzled‐4 (FZD4) by 61%, when the osteocytes were stimulated with flow. The invasion of these breast cancer cells across Matrigel was also reduced by 47%, and this difference was abolished by MMP‐9 inhibitors. In conclusion, we demonstrated that flow‐stimulated osteocytes downregulate the bone‐metastatic potential of breast cancer cells by signaling through endothelial cells. This provides insights into the capability of bone mechanical regulation in preventing bone metastases; and may assist in prescribing exercise or bone‐loading regimens to patients with breast cancers.
This article investigates the effects of mechanically stimulated osteocytes on the interaction between endothelial cells and breast cancer cells to reduce the bone‐metastatic potential. The application of conditioned medium (CM) from flow‐stimulated osteocytes to endothelial cells reduces their permeability and adhesion by breast cancer cells. The application of CM from these conditioned endothelial cells to bone‐metastatic breast cancer cells reduces their matrix metallopeptidase 9 and FZD4 expression and invasiveness.</description><subject>Adhesion</subject><subject>Antibodies</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Blood vessels</subject><subject>Bone cancer</subject><subject>Bone matrix</subject><subject>bone metastasis</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Cell adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell migration</subject><subject>Conditioning</subject><subject>Endothelial cells</subject><subject>Fluid dynamics</subject><subject>Fluid flow</subject><subject>Frizzled protein</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Intercellular adhesion molecule 1</subject><subject>invasion</subject><subject>matrix metallopeptidase 9 (MMP‐9)</subject><subject>Mechanical loading</subject><subject>Metalloproteinase</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Mimicry</subject><subject>osteocyte</subject><subject>Osteocytes</subject><subject>Patients</subject><subject>Permeability</subject><subject>Quality of life</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA sequencing</subject><subject>Signaling</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kctu2zAQRYmiReOmXfQHCgLdJAslw4csadkaeRUJumnXAkWNbBoU6ZJUA-_yCfnGfEnpOMmiQFYDDA4O5s4l5DODEwbAT9e6O-E1CPmGzBg0VSHnUr4lM6gEFFwwfkA-xLgGgKYR_D05ECBZVcpmRu5vUK-UM1pZu6UxmXGyKmFPfUzo9TZhpAH7SSNNK6Sdd_hwdz9iUjGpZDTd-IQuGWWpH2gXMO-pVk5joBqtjdQ4-tek4GmX_WbplDVumWXBT8sVRdf7LLY7wSP_kbwblI346Wkekt_nZ78Wl8X1z4urxbfrQotSyKKTQnJWl1JBztrUuoaK9V3PJB8ABW8gx-5rMcwHPe9LHCSfA5cVQ15JhUIckqO9dxP8nwljakcTdxcoh36KLWeC85ILITP69T907aeQg-yoBvIjywoydbyndPAxBhzaTTCjCtuWQburqc01tY81ZfbLk3HqRuxfyOdeMnC6B26Nxe3rpvbH4vte-Q9lvJ4c</recordid><startdate>201905</startdate><enddate>201905</enddate><creator>Ma, Yu‐Heng Vivian</creator><creator>Xu, Liangcheng</creator><creator>Mei, Xueting</creator><creator>Middleton, Kevin</creator><creator>You, Lidan</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5884-114X</orcidid></search><sort><creationdate>201905</creationdate><title>Mechanically stimulated osteocytes reduce the bone‐metastatic potential of breast cancer cells in vitro by signaling through endothelial cells</title><author>Ma, Yu‐Heng Vivian ; Xu, Liangcheng ; Mei, Xueting ; Middleton, Kevin ; You, Lidan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3534-b43421854a064498c8071dbd142f0e3290312d83f6fc6d5ef42602471e274ae33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adhesion</topic><topic>Antibodies</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Blood vessels</topic><topic>Bone cancer</topic><topic>Bone matrix</topic><topic>bone metastasis</topic><topic>Breast cancer</topic><topic>Cancer</topic><topic>Cell adhesion</topic><topic>Cell adhesion & migration</topic><topic>Cell migration</topic><topic>Conditioning</topic><topic>Endothelial cells</topic><topic>Fluid dynamics</topic><topic>Fluid flow</topic><topic>Frizzled protein</topic><topic>Gene expression</topic><topic>Gene sequencing</topic><topic>Intercellular adhesion molecule 1</topic><topic>invasion</topic><topic>matrix metallopeptidase 9 (MMP‐9)</topic><topic>Mechanical loading</topic><topic>Metalloproteinase</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Mimicry</topic><topic>osteocyte</topic><topic>Osteocytes</topic><topic>Patients</topic><topic>Permeability</topic><topic>Quality of life</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA sequencing</topic><topic>Signaling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Yu‐Heng Vivian</creatorcontrib><creatorcontrib>Xu, Liangcheng</creatorcontrib><creatorcontrib>Mei, Xueting</creatorcontrib><creatorcontrib>Middleton, Kevin</creatorcontrib><creatorcontrib>You, Lidan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Yu‐Heng Vivian</au><au>Xu, Liangcheng</au><au>Mei, Xueting</au><au>Middleton, Kevin</au><au>You, Lidan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanically stimulated osteocytes reduce the bone‐metastatic potential of breast cancer cells in vitro by signaling through endothelial cells</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J Cell Biochem</addtitle><date>2019-05</date><risdate>2019</risdate><volume>120</volume><issue>5</issue><spage>7590</spage><epage>7601</epage><pages>7590-7601</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Bone metastases occur in 65% to 75% of patients with advanced breast cancer and significantly worsen their survival and quality of life. We previously showed that conditioned medium (CM) from osteocytes stimulated with oscillatory fluid flow, mimicking bone mechanical loading during routine physical activities, reduced the transendothelial migration of breast cancer cells. Endothelial cells are situated at an ideal location to mediate signals between osteocytes in the bone matrix and metastasizing cancer cells in the blood vessels. In this study, we investigated the specific effects of flow‐stimulated osteocytes on the interaction between endothelial cells and breast cancer cells in vitro. We observed that CM from flow‐stimulated osteocytes reduced endothelial permeability by 15% and breast cancer cell adhesion onto endothelial monolayers by 18%. The difference in adhesion was abolished with anti‐intercellular adhesion molecule 1 (ICAM‐1) neutralizing antibodies. Furthermore, CM from endothelial cells conditioned in CM from flow‐stimulated osteocytes significantly altered the gene expression in bone‐metastatic breast cancer cells, as shown by RNA sequencing. Specifically, breast cancer cell expression of matrix metallopeptidase 9 (MMP‐9) was downregulated by 62%, and frizzled‐4 (FZD4) by 61%, when the osteocytes were stimulated with flow. The invasion of these breast cancer cells across Matrigel was also reduced by 47%, and this difference was abolished by MMP‐9 inhibitors. In conclusion, we demonstrated that flow‐stimulated osteocytes downregulate the bone‐metastatic potential of breast cancer cells by signaling through endothelial cells. This provides insights into the capability of bone mechanical regulation in preventing bone metastases; and may assist in prescribing exercise or bone‐loading regimens to patients with breast cancers.
This article investigates the effects of mechanically stimulated osteocytes on the interaction between endothelial cells and breast cancer cells to reduce the bone‐metastatic potential. The application of conditioned medium (CM) from flow‐stimulated osteocytes to endothelial cells reduces their permeability and adhesion by breast cancer cells. The application of CM from these conditioned endothelial cells to bone‐metastatic breast cancer cells reduces their matrix metallopeptidase 9 and FZD4 expression and invasiveness.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30417549</pmid><doi>10.1002/jcb.28034</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-5884-114X</orcidid></addata></record> |
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| subjects | Adhesion Antibodies Biocompatibility Biomedical materials Blood vessels Bone cancer Bone matrix bone metastasis Breast cancer Cancer Cell adhesion Cell adhesion & migration Cell migration Conditioning Endothelial cells Fluid dynamics Fluid flow Frizzled protein Gene expression Gene sequencing Intercellular adhesion molecule 1 invasion matrix metallopeptidase 9 (MMP‐9) Mechanical loading Metalloproteinase Metastases Metastasis Mimicry osteocyte Osteocytes Patients Permeability Quality of life Ribonucleic acid RNA RNA sequencing Signaling |
| title | Mechanically stimulated osteocytes reduce the bone‐metastatic potential of breast cancer cells in vitro by signaling through endothelial cells |
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