Tipin/Tim1/And1 protein complex promotes Pola chromatin binding and sister chromatid cohesion

The Tipin/Tim1 complex plays an important role in the S-phase checkpoint and replication fork stability. However, the biochemical function of this complex is poorly understood. Using Xenopus laevis egg extract we show that Tipin is required for DNA replication in the presence of limiting amount of r...

Full description

Saved in:
Bibliographic Details
Published in:The EMBO journal 2009-12, Vol.28 (23), p.3681-3692
Main Authors: Errico, Alessia, Cosentino, Claudia, Rivera, Teresa, Losada, Ana, Schwob, Etienne, Hunt, Tim, Costanzo, Vincenzo
Format: Article
Language:English
Subjects:
Xenopus laevis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 3692
container_issue 23
container_start_page 3681
container_title The EMBO journal
container_volume 28
creator Errico, Alessia
Cosentino, Claudia
Rivera, Teresa
Losada, Ana
Schwob, Etienne
Hunt, Tim
Costanzo, Vincenzo
description The Tipin/Tim1 complex plays an important role in the S-phase checkpoint and replication fork stability. However, the biochemical function of this complex is poorly understood. Using Xenopus laevis egg extract we show that Tipin is required for DNA replication in the presence of limiting amount of replication origins. Under these conditions the DNA replication defect correlates with decreased levels of DNA Pola on chromatin. We identified And1, a Pola chromatin-loading factor, as new Tipin-binding partner. We found that both Tipin and And1 promote stable binding of Pola to chromatin and that this is required for DNA replication under unchallenged conditions. Strikingly, extracts lacking Tipin and And1 also show reduced sister chromatids cohesion. These data indicate that Tipin/Tim1/And1 form a complex that links stabilization of replication fork and establishment of sister chromatid cohesion.
doi_str_mv 10.1038/emboj.2009.304
format article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_21261654</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>21261654</sourcerecordid><originalsourceid>FETCH-proquest_miscellaneous_212616543</originalsourceid><addsrcrecordid>eNqNTrsKwkAQvELBZ2t9lZ3JXhI1liKKpUVaCWey6so9ohvBz_cEsbYa5skIMVEQKUjzGO3J36IEYBWlkHVEH5KFmmUqX_XEgPkGAPN8qfriWFBDLi7IqnjtaiWbh2-RnKy8bQy-PtwGheXBGy2ra6C6Df6JXE3uIrWrJRO3-PiZdShfkcm7keietWEcf3EoprttsdnPwur9idyWlrhCY7RD_-QyUeHmYp6lfwffOjVMAA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>21261654</pqid></control><display><type>article</type><title>Tipin/Tim1/And1 protein complex promotes Pola chromatin binding and sister chromatid cohesion</title><source>Wiley-Blackwell Journals</source><source>PubMed Central</source><creator>Errico, Alessia ; Cosentino, Claudia ; Rivera, Teresa ; Losada, Ana ; Schwob, Etienne ; Hunt, Tim ; Costanzo, Vincenzo</creator><creatorcontrib>Errico, Alessia ; Cosentino, Claudia ; Rivera, Teresa ; Losada, Ana ; Schwob, Etienne ; Hunt, Tim ; Costanzo, Vincenzo</creatorcontrib><description>The Tipin/Tim1 complex plays an important role in the S-phase checkpoint and replication fork stability. However, the biochemical function of this complex is poorly understood. Using Xenopus laevis egg extract we show that Tipin is required for DNA replication in the presence of limiting amount of replication origins. Under these conditions the DNA replication defect correlates with decreased levels of DNA Pola on chromatin. We identified And1, a Pola chromatin-loading factor, as new Tipin-binding partner. We found that both Tipin and And1 promote stable binding of Pola to chromatin and that this is required for DNA replication under unchallenged conditions. Strikingly, extracts lacking Tipin and And1 also show reduced sister chromatids cohesion. These data indicate that Tipin/Tim1/And1 form a complex that links stabilization of replication fork and establishment of sister chromatid cohesion.</description><identifier>ISSN: 0261-4189</identifier><identifier>DOI: 10.1038/emboj.2009.304</identifier><language>eng</language><subject>Xenopus laevis</subject><ispartof>The EMBO journal, 2009-12, Vol.28 (23), p.3681-3692</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids></links><search><creatorcontrib>Errico, Alessia</creatorcontrib><creatorcontrib>Cosentino, Claudia</creatorcontrib><creatorcontrib>Rivera, Teresa</creatorcontrib><creatorcontrib>Losada, Ana</creatorcontrib><creatorcontrib>Schwob, Etienne</creatorcontrib><creatorcontrib>Hunt, Tim</creatorcontrib><creatorcontrib>Costanzo, Vincenzo</creatorcontrib><title>Tipin/Tim1/And1 protein complex promotes Pola chromatin binding and sister chromatid cohesion</title><title>The EMBO journal</title><description>The Tipin/Tim1 complex plays an important role in the S-phase checkpoint and replication fork stability. However, the biochemical function of this complex is poorly understood. Using Xenopus laevis egg extract we show that Tipin is required for DNA replication in the presence of limiting amount of replication origins. Under these conditions the DNA replication defect correlates with decreased levels of DNA Pola on chromatin. We identified And1, a Pola chromatin-loading factor, as new Tipin-binding partner. We found that both Tipin and And1 promote stable binding of Pola to chromatin and that this is required for DNA replication under unchallenged conditions. Strikingly, extracts lacking Tipin and And1 also show reduced sister chromatids cohesion. These data indicate that Tipin/Tim1/And1 form a complex that links stabilization of replication fork and establishment of sister chromatid cohesion.</description><subject>Xenopus laevis</subject><issn>0261-4189</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqNTrsKwkAQvELBZ2t9lZ3JXhI1liKKpUVaCWey6so9ohvBz_cEsbYa5skIMVEQKUjzGO3J36IEYBWlkHVEH5KFmmUqX_XEgPkGAPN8qfriWFBDLi7IqnjtaiWbh2-RnKy8bQy-PtwGheXBGy2ra6C6Df6JXE3uIrWrJRO3-PiZdShfkcm7keietWEcf3EoprttsdnPwur9idyWlrhCY7RD_-QyUeHmYp6lfwffOjVMAA</recordid><startdate>20091202</startdate><enddate>20091202</enddate><creator>Errico, Alessia</creator><creator>Cosentino, Claudia</creator><creator>Rivera, Teresa</creator><creator>Losada, Ana</creator><creator>Schwob, Etienne</creator><creator>Hunt, Tim</creator><creator>Costanzo, Vincenzo</creator><scope>7TM</scope></search><sort><creationdate>20091202</creationdate><title>Tipin/Tim1/And1 protein complex promotes Pola chromatin binding and sister chromatid cohesion</title><author>Errico, Alessia ; Cosentino, Claudia ; Rivera, Teresa ; Losada, Ana ; Schwob, Etienne ; Hunt, Tim ; Costanzo, Vincenzo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_212616543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Errico, Alessia</creatorcontrib><creatorcontrib>Cosentino, Claudia</creatorcontrib><creatorcontrib>Rivera, Teresa</creatorcontrib><creatorcontrib>Losada, Ana</creatorcontrib><creatorcontrib>Schwob, Etienne</creatorcontrib><creatorcontrib>Hunt, Tim</creatorcontrib><creatorcontrib>Costanzo, Vincenzo</creatorcontrib><collection>Nucleic Acids Abstracts</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Errico, Alessia</au><au>Cosentino, Claudia</au><au>Rivera, Teresa</au><au>Losada, Ana</au><au>Schwob, Etienne</au><au>Hunt, Tim</au><au>Costanzo, Vincenzo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tipin/Tim1/And1 protein complex promotes Pola chromatin binding and sister chromatid cohesion</atitle><jtitle>The EMBO journal</jtitle><date>2009-12-02</date><risdate>2009</risdate><volume>28</volume><issue>23</issue><spage>3681</spage><epage>3692</epage><pages>3681-3692</pages><issn>0261-4189</issn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>content type line 23</notes><notes>ObjectType-Feature-2</notes><abstract>The Tipin/Tim1 complex plays an important role in the S-phase checkpoint and replication fork stability. However, the biochemical function of this complex is poorly understood. Using Xenopus laevis egg extract we show that Tipin is required for DNA replication in the presence of limiting amount of replication origins. Under these conditions the DNA replication defect correlates with decreased levels of DNA Pola on chromatin. We identified And1, a Pola chromatin-loading factor, as new Tipin-binding partner. We found that both Tipin and And1 promote stable binding of Pola to chromatin and that this is required for DNA replication under unchallenged conditions. Strikingly, extracts lacking Tipin and And1 also show reduced sister chromatids cohesion. These data indicate that Tipin/Tim1/And1 form a complex that links stabilization of replication fork and establishment of sister chromatid cohesion.</abstract><doi>10.1038/emboj.2009.304</doi></addata></record>
fulltext fulltext
identifier ISSN: 0261-4189
ispartof The EMBO journal, 2009-12, Vol.28 (23), p.3681-3692
issn 0261-4189
language eng
recordid cdi_proquest_miscellaneous_21261654
source Wiley-Blackwell Journals; PubMed Central
subjects Xenopus laevis
title Tipin/Tim1/And1 protein complex promotes Pola chromatin binding and sister chromatid cohesion
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T20%3A30%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Tipin/Tim1/And1%20protein%20complex%20promotes%20Pola%20chromatin%20binding%20and%20sister%20chromatid%20cohesion&rft.jtitle=The%20EMBO%20journal&rft.au=Errico,%20Alessia&rft.date=2009-12-02&rft.volume=28&rft.issue=23&rft.spage=3681&rft.epage=3692&rft.pages=3681-3692&rft.issn=0261-4189&rft_id=info:doi/10.1038/emboj.2009.304&rft_dat=%3Cproquest%3E21261654%3C/proquest%3E%3Cgrp_id%3Ecdi_FETCH-proquest_miscellaneous_212616543%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=21261654&rft_id=info:pmid/&rfr_iscdi=true