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Molecular identification and microscopic characterization of poxvirus in a Guiana dolphin and a common bottlenose dolphin, Brazil
The poxviruses identified in cetaceans are associated with characteristic tattoo or ring skin lesions. However, little is known regarding the prevalence and progression of these lesions and the molecular characterization of cetacean poxviruses in the Southern Hemisphere. This manuscript describes th...
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| Published in: | Diseases of aquatic organisms 2018-09, Vol.130 (3), p.177-185 |
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| creator | Sacristán, Carlos Esperón, Fernando Marigo, Juliana Ewbank, Ana Carolina de Carvalho, Rafael Ramos Groch, Kátia Regina de Castilho, Pedro Volkmer Sánchez-Sarmiento, Angélica María Costa-Silva, Samira Ferreira-Machado, Eduardo Gonzales-Viera, Omar Antonio Daura-Jorge, Fábio G Santos-Neto, Elitieri B Lailson-Brito, José de Freitas Azevedo, Alexandre Simões-Lopes, Paulo C Neves, Carlos G Catão-Dias, José Luiz |
| description | The poxviruses identified in cetaceans are associated with characteristic tattoo or ring skin lesions. However, little is known regarding the prevalence and progression of these lesions and the molecular characterization of cetacean poxviruses in the Southern Hemisphere. This manuscript describes the progression of poxvirus-like skin lesions in 5 free-ranging Guiana dolphins Sotalia guianensis. Additionally, 151 skin samples from 113 free-ranging cetaceans from Brazil, including 4 animals with tattoo skin lesions, were selected for poxvirus testing. Poxviral DNA polymerase gene PCR amplification was used to detect the virus in β-actin-positive samples (145/151). DNA topoisomerase I gene PCR was then used in Cetaceanpoxvirus (CePV)-positive cases (n = 2), which were further evaluated by histopathology and electron microscopy. Based on photo-identification, adult Guiana dolphins presented regressing or healed poxvirus-like lesions (2/2), while juveniles presented persistent (2/3) or healed and progressive lesions (1/3). CePV DNA was amplified in a common bottlenose dolphin Tursiops truncatus and in a Guiana dolphin. Intracytoplasmic inclusion bodies and viral particles consistent with poxvirus were identified by histology and electron microscopy, respectively. CePV-specific amino acid motifs were identified through phylogenetic analysis. Our findings corroborate previous studies that suggest the placement of poxviruses from cetaceans within the novel CePV genus. This is the first molecular identification of poxvirus in South American odontocetes. |
| doi_str_mv | 10.3354/dao03271 |
| format | article |
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However, little is known regarding the prevalence and progression of these lesions and the molecular characterization of cetacean poxviruses in the Southern Hemisphere. This manuscript describes the progression of poxvirus-like skin lesions in 5 free-ranging Guiana dolphins Sotalia guianensis. Additionally, 151 skin samples from 113 free-ranging cetaceans from Brazil, including 4 animals with tattoo skin lesions, were selected for poxvirus testing. Poxviral DNA polymerase gene PCR amplification was used to detect the virus in β-actin-positive samples (145/151). DNA topoisomerase I gene PCR was then used in Cetaceanpoxvirus (CePV)-positive cases (n = 2), which were further evaluated by histopathology and electron microscopy. Based on photo-identification, adult Guiana dolphins presented regressing or healed poxvirus-like lesions (2/2), while juveniles presented persistent (2/3) or healed and progressive lesions (1/3). CePV DNA was amplified in a common bottlenose dolphin Tursiops truncatus and in a Guiana dolphin. Intracytoplasmic inclusion bodies and viral particles consistent with poxvirus were identified by histology and electron microscopy, respectively. CePV-specific amino acid motifs were identified through phylogenetic analysis. Our findings corroborate previous studies that suggest the placement of poxviruses from cetaceans within the novel CePV genus. This is the first molecular identification of poxvirus in South American odontocetes.</description><identifier>ISSN: 0177-5103</identifier><identifier>EISSN: 1616-1580</identifier><identifier>DOI: 10.3354/dao03271</identifier><identifier>PMID: 30259870</identifier><language>eng</language><publisher>Germany: Inter-Research Science Center</publisher><subject>Actin ; Amino acids ; Amplification ; Aquatic mammals ; Cetacea ; Deoxyribonucleic acid ; DNA ; DNA polymerase ; DNA topoisomerase ; DNA-directed DNA polymerase ; Dolphins ; Dolphins & porpoises ; Electron microscopy ; Histology ; Histopathology ; Inclusion bodies ; Lesions ; Marine mammals ; Microscopy ; Phylogeny ; Polymerase chain reaction ; Regression analysis ; Skin diseases ; Sotalia guianensis ; Southern Hemisphere ; Tursiops truncatus ; Viruses</subject><ispartof>Diseases of aquatic organisms, 2018-09, Vol.130 (3), p.177-185</ispartof><rights>Copyright Inter-Research Science Center 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-2ecb96f4fe34cbd3dd7cca40fe4395a3faa69af4517dafe5fc1c69152b9b8bed3</citedby><cites>FETCH-LOGICAL-c345t-2ecb96f4fe34cbd3dd7cca40fe4395a3faa69af4517dafe5fc1c69152b9b8bed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30259870$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sacristán, Carlos</creatorcontrib><creatorcontrib>Esperón, Fernando</creatorcontrib><creatorcontrib>Marigo, Juliana</creatorcontrib><creatorcontrib>Ewbank, Ana Carolina</creatorcontrib><creatorcontrib>de Carvalho, Rafael Ramos</creatorcontrib><creatorcontrib>Groch, Kátia Regina</creatorcontrib><creatorcontrib>de Castilho, Pedro Volkmer</creatorcontrib><creatorcontrib>Sánchez-Sarmiento, Angélica María</creatorcontrib><creatorcontrib>Costa-Silva, Samira</creatorcontrib><creatorcontrib>Ferreira-Machado, Eduardo</creatorcontrib><creatorcontrib>Gonzales-Viera, Omar Antonio</creatorcontrib><creatorcontrib>Daura-Jorge, Fábio G</creatorcontrib><creatorcontrib>Santos-Neto, Elitieri B</creatorcontrib><creatorcontrib>Lailson-Brito, José</creatorcontrib><creatorcontrib>de Freitas Azevedo, Alexandre</creatorcontrib><creatorcontrib>Simões-Lopes, Paulo C</creatorcontrib><creatorcontrib>Neves, Carlos G</creatorcontrib><creatorcontrib>Catão-Dias, José Luiz</creatorcontrib><title>Molecular identification and microscopic characterization of poxvirus in a Guiana dolphin and a common bottlenose dolphin, Brazil</title><title>Diseases of aquatic organisms</title><addtitle>Dis Aquat Organ</addtitle><description>The poxviruses identified in cetaceans are associated with characteristic tattoo or ring skin lesions. However, little is known regarding the prevalence and progression of these lesions and the molecular characterization of cetacean poxviruses in the Southern Hemisphere. This manuscript describes the progression of poxvirus-like skin lesions in 5 free-ranging Guiana dolphins Sotalia guianensis. Additionally, 151 skin samples from 113 free-ranging cetaceans from Brazil, including 4 animals with tattoo skin lesions, were selected for poxvirus testing. Poxviral DNA polymerase gene PCR amplification was used to detect the virus in β-actin-positive samples (145/151). DNA topoisomerase I gene PCR was then used in Cetaceanpoxvirus (CePV)-positive cases (n = 2), which were further evaluated by histopathology and electron microscopy. Based on photo-identification, adult Guiana dolphins presented regressing or healed poxvirus-like lesions (2/2), while juveniles presented persistent (2/3) or healed and progressive lesions (1/3). CePV DNA was amplified in a common bottlenose dolphin Tursiops truncatus and in a Guiana dolphin. Intracytoplasmic inclusion bodies and viral particles consistent with poxvirus were identified by histology and electron microscopy, respectively. CePV-specific amino acid motifs were identified through phylogenetic analysis. Our findings corroborate previous studies that suggest the placement of poxviruses from cetaceans within the novel CePV genus. 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However, little is known regarding the prevalence and progression of these lesions and the molecular characterization of cetacean poxviruses in the Southern Hemisphere. This manuscript describes the progression of poxvirus-like skin lesions in 5 free-ranging Guiana dolphins Sotalia guianensis. Additionally, 151 skin samples from 113 free-ranging cetaceans from Brazil, including 4 animals with tattoo skin lesions, were selected for poxvirus testing. Poxviral DNA polymerase gene PCR amplification was used to detect the virus in β-actin-positive samples (145/151). DNA topoisomerase I gene PCR was then used in Cetaceanpoxvirus (CePV)-positive cases (n = 2), which were further evaluated by histopathology and electron microscopy. Based on photo-identification, adult Guiana dolphins presented regressing or healed poxvirus-like lesions (2/2), while juveniles presented persistent (2/3) or healed and progressive lesions (1/3). CePV DNA was amplified in a common bottlenose dolphin Tursiops truncatus and in a Guiana dolphin. Intracytoplasmic inclusion bodies and viral particles consistent with poxvirus were identified by histology and electron microscopy, respectively. CePV-specific amino acid motifs were identified through phylogenetic analysis. Our findings corroborate previous studies that suggest the placement of poxviruses from cetaceans within the novel CePV genus. This is the first molecular identification of poxvirus in South American odontocetes.</abstract><cop>Germany</cop><pub>Inter-Research Science Center</pub><pmid>30259870</pmid><doi>10.3354/dao03271</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0177-5103 |
| ispartof | Diseases of aquatic organisms, 2018-09, Vol.130 (3), p.177-185 |
| issn | 0177-5103 1616-1580 |
| language | eng |
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| source | Science Journals (Open access) |
| subjects | Actin Amino acids Amplification Aquatic mammals Cetacea Deoxyribonucleic acid DNA DNA polymerase DNA topoisomerase DNA-directed DNA polymerase Dolphins Dolphins & porpoises Electron microscopy Histology Histopathology Inclusion bodies Lesions Marine mammals Microscopy Phylogeny Polymerase chain reaction Regression analysis Skin diseases Sotalia guianensis Southern Hemisphere Tursiops truncatus Viruses |
| title | Molecular identification and microscopic characterization of poxvirus in a Guiana dolphin and a common bottlenose dolphin, Brazil |
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