Simultaneous co-immobilization of three enzymes onto a modified glassy carbon electrode to fabricate a high-performance amperometric biosensor for determination of total cholesterol

In this work, we have fabricated a novel amperometric cholesterol (CHO) biosensor because of the importance of determination of CHO levels in blood which is an important parameter for diagnosis and prevention of disease. To achieve this goal, cholesterol oxidase, cholesterol esterase and horseradish...

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Bibliographic Details
Published in:International journal of biological macromolecules 2018-12, Vol.120 (Pt A), p.587-595
Main Authors: Rashidi, Khodabakhsh, Mahmoudi, Majid, Mohammadi, Ghobad, Zangeneh, Mohammad Mahdi, Korani, Shahla, Goicoechea, Hector C., Gu, Hui-Wen, Jalalvand, Ali R.
Format: Article
Language:English
Subjects:
Animals
Benzoates - chemistry
Biosensing Techniques - instrumentation
Biosensor
Chitin - chemistry
Cholesterol
Cholesterol - blood
Cholesterol Oxidase - chemistry
Electrochemical Techniques - methods
Electrodes
Enzymes, Immobilized - chemistry
Ferrous Compounds - chemistry
Glass - chemistry
Gold - chemistry
Horseradish Peroxidase - chemistry
Limit of Detection
Metal Nanoparticles - chemistry
Nanotubes, Carbon - chemistry
Pyrroles - chemistry
Rats
Rats plasma
Reproducibility of Results
Sterol Esterase - chemistry
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Summary:In this work, we have fabricated a novel amperometric cholesterol (CHO) biosensor because of the importance of determination of CHO levels in blood which is an important parameter for diagnosis and prevention of disease. To achieve this goal, cholesterol oxidase, cholesterol esterase and horseradish peroxidase were simultaneously co-immobilized onto a glassy carbon electrode (GCE) modified with gold nanoparticles/chitin-ionic liquid/poly(3,4-ethylenedioxypyrrole)/graphene-multiwalled carbon nanotubes-1,1′-ferrocenedicarboxylic acid-ionic liquid. Modifications applied to the bare GCE were characterized by cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. The biosensor detected CHO in linear ranges of 0.1–25 μM and 25–950 μM with a detection limit of 0.07 μM. The sensitivity of the biosensor was estimated to be 6.6 μA μM−1 cm−2, its response time was
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2018.08.163