Three-Dimensional Plasmonic Trap Array for Ultrasensitive Surface-Enhanced Raman Scattering Analysis of Single Cells

Single-cell analysis provides an important strategy to evaluate cellular heterogeneity. Although surface-enhanced Raman scattering (SERS) has been considered as a promising label-free technique for single-cell analysis, it remains at the early stage for characterizing the extracellular metabolites o...

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Published in:Analytical chemistry (Washington) 2018-09, Vol.90 (17), p.10394-10399
Main Authors: Yao, Yuanyuan, Ji, Ji, Zhang, Hongding, Zhang, Kun, Liu, Baohong, Yang, Pengyuan
Format: Article
Language:English
Subjects:
Biomarkers
Cells
Chemistry
Droplets
Heterogeneity
Metabolites
Microstructure
Nanoparticles
Polydimethylsiloxane
Raman spectra
Scattering
Silicone resins
Silver
Spores
Surface plasmon resonance
Traps
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cited_by cdi_FETCH-LOGICAL-a376t-1e57abc258b8ed5adc1c4263aae99015ef9faacab013a1d865f3e17668f967a93
cites cdi_FETCH-LOGICAL-a376t-1e57abc258b8ed5adc1c4263aae99015ef9faacab013a1d865f3e17668f967a93
container_end_page 10399
container_issue 17
container_start_page 10394
container_title Analytical chemistry (Washington)
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creator Yao, Yuanyuan
Ji, Ji
Zhang, Hongding
Zhang, Kun
Liu, Baohong
Yang, Pengyuan
description Single-cell analysis provides an important strategy to evaluate cellular heterogeneity. Although surface-enhanced Raman scattering (SERS) has been considered as a promising label-free technique for single-cell analysis, it remains at the early stage for characterizing the extracellular metabolites of single cells. Herein, we developed a convenient, flexible, and straightforward three-dimensional (3D) plasmonic trap array for simultaneously compartmentalizing and sensitively detecting single-cell metabolites. The 3D trap was spontaneously self-formed by an interfacial-energy-driven process when a liquid droplet was covered with an immiscible oil liquid (polydimethylsiloxane, PDMS). When a droplet of pure AgNO3 solution was immersed into PDMS, Ag+ ions were automatically reduced by the residual Si–H groups in PDMS. Snowflake-like nanoparticles of Ag could be formed on the inner surface of the 3D traps by tuning the concentration of Ag salt precursors and then assembled to flower-like microstructures, endowing the traps with remarkable plasmon enhancement. The established 3D traps exhibited considerably enhanced surface plasmon resonance signals for Raman reporting, and a low detection limit at the aM level was achieved for p-aminothiophenol. Moreover, these 3D traps can serve as an efficient tool for single-cell SERS measurement. As a proof-of-concept, dipicolinic acid, a common biomarker of bacterial spores, was successfully detected from a single cell. The presented approach provides a versatile tool for label-free and sensitive detection of single-cell environments.
doi_str_mv 10.1021/acs.analchem.8b02252
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The established 3D traps exhibited considerably enhanced surface plasmon resonance signals for Raman reporting, and a low detection limit at the aM level was achieved for p-aminothiophenol. Moreover, these 3D traps can serve as an efficient tool for single-cell SERS measurement. As a proof-of-concept, dipicolinic acid, a common biomarker of bacterial spores, was successfully detected from a single cell. 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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Biomarkers
Cells
Chemistry
Droplets
Heterogeneity
Metabolites
Microstructure
Nanoparticles
Polydimethylsiloxane
Raman spectra
Scattering
Silicone resins
Silver
Spores
Surface plasmon resonance
Traps
title Three-Dimensional Plasmonic Trap Array for Ultrasensitive Surface-Enhanced Raman Scattering Analysis of Single Cells
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