Establishment of cardiac remodeling model in FVB/N mice by intraperitoneal injection of isoproterenol

To explore the feasibility of intraperitoneal injection of isoproterenol (ISO) to induce cardiac remodeling in FVB/N mice. Forty-eight FVB/N mice were divided into back subcutaneous saline group (subcutaneous saline group), intraperitoneal saline group, back subcutaneous ISO group (subcutaneous ISO...

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Published in:Zhongguo dang dai er ke za zhi 2018-06, Vol.20 (6), p.508
Main Authors: Yuan, Yong-Hua, Zheng, Xue-Ming, He, Xue-Hua, Liu, Li-Ping, Xu, Wei, Xia, Xiao-Hui, Luo, Jian-Hong, Lyu, Mei, Zhu, Qian-Li, Wang, Sheng, Wu, Shi
Format: Article
Language:Chinese
Subjects:
Animals
Atrial Remodeling - drug effects
Cardiovascular Diseases - drug therapy
Cardiovascular Diseases - metabolism
Cardiovascular Diseases - pathology
Cardiovascular Diseases - physiopathology
Collagen - metabolism
Disease Models, Animal
Humans
Injections, Intraperitoneal
Isoproterenol - administration & dosage
Male
Mice
Myocardium - metabolism
Myocardium - pathology
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Summary:To explore the feasibility of intraperitoneal injection of isoproterenol (ISO) to induce cardiac remodeling in FVB/N mice. Forty-eight FVB/N mice were divided into back subcutaneous saline group (subcutaneous saline group), intraperitoneal saline group, back subcutaneous ISO group (subcutaneous ISO group), and intraperitoneal ISO group according to the route of administration of saline or ISO. ISO (30 μg/g body weight/day) was given to the subcutaneous ISO group and the intraperitoneal ISO group, twice daily with an interval of 12 hours, for 14 consecutive days. The subcutaneous saline group and the intraperitoneal saline group were injected with an equal volume of saline. The left ventricular end-diastolic posterior wall thickness was measured by echocardiography, and the ratio of heart weight to tibia length was determined. Hematoxylin-eosin staining was used to determine the myocardial fiber diameter. Picric-sirius red staining was used to determine the myocardial collagen deposition area. Quantitative rea
ISSN:1008-8830
DOI:10.7499/j.issn.1008-8830.2018.06.015