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Metagenomic analysis of oral microbiome in young children aged 6–8 years living in a rural isolated Chinese province
Background The mixed dentition is an important transition period from primary teeth to permanent teeth. However, the caries prevalence of first permanent molar in mixed dentitions was about 30%, which almost represent the caries rate of permanent teeth in this period of time. Therefore, we assessed...
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| Published in: | Oral diseases 2018-09, Vol.24 (6), p.1115-1125 |
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| container_title | Oral diseases |
| container_volume | 24 |
| creator | Xu, Y Jia, YH Chen, L Huang, WM Yang, DQ |
| description | Background
The mixed dentition is an important transition period from primary teeth to permanent teeth. However, the caries prevalence of first permanent molar in mixed dentitions was about 30%, which almost represent the caries rate of permanent teeth in this period of time. Therefore, we assessed the oral bacterial profiles in young children (age 6–8) with mixed dentition with or without first molar caries for providing the research basis of caries etiology.
Methods
We collected samples of supragingival plaque and saliva from the children living in Guizhou, a rural isolated province in China. Then, we performed DNA extraction and purification followed by 454 pyrosequencing of the V1–V3 hypervariable regions of the 16S rRNA and compared our results with those of previous research.
Results
(i) We analyzed 48,320 unique sequences that represented 18 phyla, 29 classes, 44 orders, 74 families, 129 genera, 15,003 species‐level OUT in plaque and saliva samples; (ii) longitudinally, there was the “healthy core microbiome” between healthy deciduous dentition and early mixed dentition, for example, Neisseria, Porphyromonas, Selenomonas etc.; (iii) horizontally, there also existed the “healthy core microbiome” in early mixed dentition, for example, Neisseria, Streptococcus, Prevotella etc.; (iv) the dominant bacteria detected by Lefse in caries group including Actinomycetaceae, Streptobacillus (p |
| doi_str_mv | 10.1111/odi.12871 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2027070122</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2027070122</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4191-4d07f2675eea4b7125d4ebe205b0c689d8c1f4067acfaa8868bd78d7881adf1f3</originalsourceid><addsrcrecordid>eNp10UFL5DAUB_AgKzuj7sEvIIG9rIdqkmmT9Ciz6yqMeFHwVtLmVSNpMyZTpbf9Dn5DP8m-cdSDYAgkhN_7B94jZJ-zI47rOFh3xIVWfItMuWQ8Y1oU3_A-K_KsELObCdlJ6Z4xrsqZ-E4mopRSCZlPyeMFrMwt9KFzDTW98WNyiYaWhmg8xccYahc6oK6nYxj6W9rcOW8j9BTLLJUv_541HcHERL17dAhQGhqHdb1LwZsVsvmd6yEBXcaApoE9st0an-DH27lLrk__XM3PssXl3_P5ySJrcl7yLLdMtUKqAsDkteKisDnUIFhRs0bq0uqGtzmTyjStMVpLXVulcWtubMvb2S75tcnFjx8GSKuqc6kB700PYUiVYEIxxbgQSH9-ovdhiNiRtSq5LLViOarDjcLGpBShrZbRdSaOFWfVehgVDqN6HQbag7fEoe7Afsj37iM43oAn52H8Oqm6_H2-ifwPdAqUyQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2091698704</pqid></control><display><type>article</type><title>Metagenomic analysis of oral microbiome in young children aged 6–8 years living in a rural isolated Chinese province</title><source>Wiley-Blackwell Read & Publish Collection</source><creator>Xu, Y ; Jia, YH ; Chen, L ; Huang, WM ; Yang, DQ</creator><creatorcontrib>Xu, Y ; Jia, YH ; Chen, L ; Huang, WM ; Yang, DQ</creatorcontrib><description>Background
The mixed dentition is an important transition period from primary teeth to permanent teeth. However, the caries prevalence of first permanent molar in mixed dentitions was about 30%, which almost represent the caries rate of permanent teeth in this period of time. Therefore, we assessed the oral bacterial profiles in young children (age 6–8) with mixed dentition with or without first molar caries for providing the research basis of caries etiology.
Methods
We collected samples of supragingival plaque and saliva from the children living in Guizhou, a rural isolated province in China. Then, we performed DNA extraction and purification followed by 454 pyrosequencing of the V1–V3 hypervariable regions of the 16S rRNA and compared our results with those of previous research.
Results
(i) We analyzed 48,320 unique sequences that represented 18 phyla, 29 classes, 44 orders, 74 families, 129 genera, 15,003 species‐level OUT in plaque and saliva samples; (ii) longitudinally, there was the “healthy core microbiome” between healthy deciduous dentition and early mixed dentition, for example, Neisseria, Porphyromonas, Selenomonas etc.; (iii) horizontally, there also existed the “healthy core microbiome” in early mixed dentition, for example, Neisseria, Streptococcus, Prevotella etc.; (iv) the dominant bacteria detected by Lefse in caries group including Actinomycetaceae, Streptobacillus (p < 0.05) and those in caries‐free group including Gammaproteobacteria, Pasteurellaceae, Aggregatibacter, Chloroflexi, (p < 0.05).
Conclusions
The oral cavity is a highly heterogeneous ecosystem with the “healthy core microbiome” in children, although microbial composition shifts along with aging. In addition, the abundance and diversity of microbiota vary between caries and caries‐free groups verify the ecological plaque hypothesis.</description><identifier>ISSN: 1354-523X</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.12871</identifier><identifier>PMID: 29667264</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>454 pyrosequencing ; Aging ; Child ; Children ; China ; Dental caries ; Dental plaque ; Dentistry ; Dentition ; Dentition, Mixed ; Deoxyribonucleic acid ; DNA ; Etiology ; Female ; healthy core microbiome ; Humans ; Male ; Metagenomics ; Microbiomes ; Microbiota ; mixed dentition ; Mouth - microbiology ; Neisseria ; Oral cavity ; plaque ; Purification ; rRNA 16S ; Rural Population ; Saliva ; Sequence Analysis, DNA ; Teeth</subject><ispartof>Oral diseases, 2018-09, Vol.24 (6), p.1115-1125</ispartof><rights>2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved</rights><rights>2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved.</rights><rights>Copyright © 2018 John Wiley & Sons A/S. Published by John Wiley &Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4191-4d07f2675eea4b7125d4ebe205b0c689d8c1f4067acfaa8868bd78d7881adf1f3</citedby><cites>FETCH-LOGICAL-c4191-4d07f2675eea4b7125d4ebe205b0c689d8c1f4067acfaa8868bd78d7881adf1f3</cites><orcidid>0000-0001-6494-6012 ; 0000-0002-8769-0117</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,27938,27939</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29667264$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Y</creatorcontrib><creatorcontrib>Jia, YH</creatorcontrib><creatorcontrib>Chen, L</creatorcontrib><creatorcontrib>Huang, WM</creatorcontrib><creatorcontrib>Yang, DQ</creatorcontrib><title>Metagenomic analysis of oral microbiome in young children aged 6–8 years living in a rural isolated Chinese province</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Background
The mixed dentition is an important transition period from primary teeth to permanent teeth. However, the caries prevalence of first permanent molar in mixed dentitions was about 30%, which almost represent the caries rate of permanent teeth in this period of time. Therefore, we assessed the oral bacterial profiles in young children (age 6–8) with mixed dentition with or without first molar caries for providing the research basis of caries etiology.
Methods
We collected samples of supragingival plaque and saliva from the children living in Guizhou, a rural isolated province in China. Then, we performed DNA extraction and purification followed by 454 pyrosequencing of the V1–V3 hypervariable regions of the 16S rRNA and compared our results with those of previous research.
Results
(i) We analyzed 48,320 unique sequences that represented 18 phyla, 29 classes, 44 orders, 74 families, 129 genera, 15,003 species‐level OUT in plaque and saliva samples; (ii) longitudinally, there was the “healthy core microbiome” between healthy deciduous dentition and early mixed dentition, for example, Neisseria, Porphyromonas, Selenomonas etc.; (iii) horizontally, there also existed the “healthy core microbiome” in early mixed dentition, for example, Neisseria, Streptococcus, Prevotella etc.; (iv) the dominant bacteria detected by Lefse in caries group including Actinomycetaceae, Streptobacillus (p < 0.05) and those in caries‐free group including Gammaproteobacteria, Pasteurellaceae, Aggregatibacter, Chloroflexi, (p < 0.05).
Conclusions
The oral cavity is a highly heterogeneous ecosystem with the “healthy core microbiome” in children, although microbial composition shifts along with aging. In addition, the abundance and diversity of microbiota vary between caries and caries‐free groups verify the ecological plaque hypothesis.</description><subject>454 pyrosequencing</subject><subject>Aging</subject><subject>Child</subject><subject>Children</subject><subject>China</subject><subject>Dental caries</subject><subject>Dental plaque</subject><subject>Dentistry</subject><subject>Dentition</subject><subject>Dentition, Mixed</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Etiology</subject><subject>Female</subject><subject>healthy core microbiome</subject><subject>Humans</subject><subject>Male</subject><subject>Metagenomics</subject><subject>Microbiomes</subject><subject>Microbiota</subject><subject>mixed dentition</subject><subject>Mouth - microbiology</subject><subject>Neisseria</subject><subject>Oral cavity</subject><subject>plaque</subject><subject>Purification</subject><subject>rRNA 16S</subject><subject>Rural Population</subject><subject>Saliva</subject><subject>Sequence Analysis, DNA</subject><subject>Teeth</subject><issn>1354-523X</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp10UFL5DAUB_AgKzuj7sEvIIG9rIdqkmmT9Ciz6yqMeFHwVtLmVSNpMyZTpbf9Dn5DP8m-cdSDYAgkhN_7B94jZJ-zI47rOFh3xIVWfItMuWQ8Y1oU3_A-K_KsELObCdlJ6Z4xrsqZ-E4mopRSCZlPyeMFrMwt9KFzDTW98WNyiYaWhmg8xccYahc6oK6nYxj6W9rcOW8j9BTLLJUv_541HcHERL17dAhQGhqHdb1LwZsVsvmd6yEBXcaApoE9st0an-DH27lLrk__XM3PssXl3_P5ySJrcl7yLLdMtUKqAsDkteKisDnUIFhRs0bq0uqGtzmTyjStMVpLXVulcWtubMvb2S75tcnFjx8GSKuqc6kB700PYUiVYEIxxbgQSH9-ovdhiNiRtSq5LLViOarDjcLGpBShrZbRdSaOFWfVehgVDqN6HQbag7fEoe7Afsj37iM43oAn52H8Oqm6_H2-ifwPdAqUyQ</recordid><startdate>201809</startdate><enddate>201809</enddate><creator>Xu, Y</creator><creator>Jia, YH</creator><creator>Chen, L</creator><creator>Huang, WM</creator><creator>Yang, DQ</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6494-6012</orcidid><orcidid>https://orcid.org/0000-0002-8769-0117</orcidid></search><sort><creationdate>201809</creationdate><title>Metagenomic analysis of oral microbiome in young children aged 6–8 years living in a rural isolated Chinese province</title><author>Xu, Y ; Jia, YH ; Chen, L ; Huang, WM ; Yang, DQ</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4191-4d07f2675eea4b7125d4ebe205b0c689d8c1f4067acfaa8868bd78d7881adf1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>454 pyrosequencing</topic><topic>Aging</topic><topic>Child</topic><topic>Children</topic><topic>China</topic><topic>Dental caries</topic><topic>Dental plaque</topic><topic>Dentistry</topic><topic>Dentition</topic><topic>Dentition, Mixed</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Etiology</topic><topic>Female</topic><topic>healthy core microbiome</topic><topic>Humans</topic><topic>Male</topic><topic>Metagenomics</topic><topic>Microbiomes</topic><topic>Microbiota</topic><topic>mixed dentition</topic><topic>Mouth - microbiology</topic><topic>Neisseria</topic><topic>Oral cavity</topic><topic>plaque</topic><topic>Purification</topic><topic>rRNA 16S</topic><topic>Rural Population</topic><topic>Saliva</topic><topic>Sequence Analysis, DNA</topic><topic>Teeth</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Y</creatorcontrib><creatorcontrib>Jia, YH</creatorcontrib><creatorcontrib>Chen, L</creatorcontrib><creatorcontrib>Huang, WM</creatorcontrib><creatorcontrib>Yang, DQ</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Y</au><au>Jia, YH</au><au>Chen, L</au><au>Huang, WM</au><au>Yang, DQ</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metagenomic analysis of oral microbiome in young children aged 6–8 years living in a rural isolated Chinese province</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2018-09</date><risdate>2018</risdate><volume>24</volume><issue>6</issue><spage>1115</spage><epage>1125</epage><pages>1115-1125</pages><issn>1354-523X</issn><eissn>1601-0825</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Background
The mixed dentition is an important transition period from primary teeth to permanent teeth. However, the caries prevalence of first permanent molar in mixed dentitions was about 30%, which almost represent the caries rate of permanent teeth in this period of time. Therefore, we assessed the oral bacterial profiles in young children (age 6–8) with mixed dentition with or without first molar caries for providing the research basis of caries etiology.
Methods
We collected samples of supragingival plaque and saliva from the children living in Guizhou, a rural isolated province in China. Then, we performed DNA extraction and purification followed by 454 pyrosequencing of the V1–V3 hypervariable regions of the 16S rRNA and compared our results with those of previous research.
Results
(i) We analyzed 48,320 unique sequences that represented 18 phyla, 29 classes, 44 orders, 74 families, 129 genera, 15,003 species‐level OUT in plaque and saliva samples; (ii) longitudinally, there was the “healthy core microbiome” between healthy deciduous dentition and early mixed dentition, for example, Neisseria, Porphyromonas, Selenomonas etc.; (iii) horizontally, there also existed the “healthy core microbiome” in early mixed dentition, for example, Neisseria, Streptococcus, Prevotella etc.; (iv) the dominant bacteria detected by Lefse in caries group including Actinomycetaceae, Streptobacillus (p < 0.05) and those in caries‐free group including Gammaproteobacteria, Pasteurellaceae, Aggregatibacter, Chloroflexi, (p < 0.05).
Conclusions
The oral cavity is a highly heterogeneous ecosystem with the “healthy core microbiome” in children, although microbial composition shifts along with aging. In addition, the abundance and diversity of microbiota vary between caries and caries‐free groups verify the ecological plaque hypothesis.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29667264</pmid><doi>10.1111/odi.12871</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-6494-6012</orcidid><orcidid>https://orcid.org/0000-0002-8769-0117</orcidid></addata></record> |
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| ispartof | Oral diseases, 2018-09, Vol.24 (6), p.1115-1125 |
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| subjects | 454 pyrosequencing Aging Child Children China Dental caries Dental plaque Dentistry Dentition Dentition, Mixed Deoxyribonucleic acid DNA Etiology Female healthy core microbiome Humans Male Metagenomics Microbiomes Microbiota mixed dentition Mouth - microbiology Neisseria Oral cavity plaque Purification rRNA 16S Rural Population Saliva Sequence Analysis, DNA Teeth |
| title | Metagenomic analysis of oral microbiome in young children aged 6–8 years living in a rural isolated Chinese province |
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