Biosynthesis and Characterization of Copper Nanoparticles Using Shewanella oneidensis: Application for Click Chemistry

Copper nanoparticles (Cu‐NPs) have a wide range of applications as heterogeneous catalysts. In this study, a novel green biosynthesis route for producing Cu‐NPs using the metal‐reducing bacterium, Shewanella oneidensis is demonstrated. Thin section transmission electron microscopy shows that the Cu‐...

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Bibliographic Details
Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2018-03, Vol.14 (10), p.n/a
Main Authors: Kimber, Richard L., Lewis, Edward A., Parmeggiani, Fabio, Smith, Kurt, Bagshaw, Heath, Starborg, Toby, Joshi, Nimisha, Figueroa, Adriana I., van der Laan, Gerrit, Cibin, Giannantonio, Gianolio, Diego, Haigh, Sarah J., Pattrick, Richard A. D., Turner, Nicholas J., Lloyd, Jonathan R.
Format: Article
Language:English
Subjects:
Absorption
Alkynes
Atomic structure
Biosynthesis
Catalysis
Catalysts
Catalytic activity
Chemical synthesis
click chemistry
Copper
copper nanoparticles
Copper oxides
Deletion
Electron energy
Electron energy loss spectroscopy
Electron microscopy
Electrons
Microscopy
Nanoparticles
Nanotechnology
Oxidation
Reduction (metal working)
Shewanella oneidensis
Surface chemistry
Surface layers
Valence
XANES
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Summary:Copper nanoparticles (Cu‐NPs) have a wide range of applications as heterogeneous catalysts. In this study, a novel green biosynthesis route for producing Cu‐NPs using the metal‐reducing bacterium, Shewanella oneidensis is demonstrated. Thin section transmission electron microscopy shows that the Cu‐NPs are predominantly intracellular and present in a typical size range of 20–40 nm. Serial block‐face scanning electron microscopy demonstrates the Cu‐NPs are well‐dispersed across the 3D structure of the cells. X‐ray absorption near‐edge spectroscopy and extended X‐ray absorption fine‐structure spectroscopy analysis show the nanoparticles are Cu(0), however, atomic resolution images and electron energy loss spectroscopy suggest partial oxidation of the surface layer to Cu2O upon exposure to air. The catalytic activity of the Cu‐NPs is demonstrated in an archetypal “click chemistry” reaction, generating good yields during azide‐alkyne cycloadditions, most likely catalyzed by the Cu(I) surface layer of the nanoparticles. Furthermore, cytochrome deletion mutants suggest a novel metal reduction system is involved in enzymatic Cu(II) reduction and Cu‐NP synthesis, which is not dependent on the Mtr pathway commonly used to reduce other high oxidation state metals in this bacterium. This work demonstrates a novel, simple, green biosynthesis method for producing efficient copper nanoparticle catalysts. A novel green biosynthesis route for the production of functional copper nanoparticles (Cu‐NPs) using a metal‐reducing bacterium is reported. Detailed characterization reveals well‐dispersed Cu(0) nanoparticles with a thin, stable Cu2O surface layer. The catalytic activity of the biogenic Cu‐NPs is demonstrated for a range of “click chemistry” azide‐alkyne cycloaddition reactions, and the commercial potential of this approach discussed.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201703145