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Characterization of the gene encoding the 16S rRNA of Enterobacter sakazakii and development of a species-specific PCR method
The gene encoding the 16S rRNA of Enterobacter ( E.) sakazakii (ATCC 29544, plus four strains isolated from powdered infant formula) was studied, and the sequence compared with those of other Enterobacteriaceae in aspects of genetic variability. Sequence differences between E. sakazakii and other En...
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| Published in: | International journal of food microbiology 2007-05, Vol.116 (2), p.214-220 |
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| cites | cdi_FETCH-LOGICAL-c460t-2e8b2676a490187b36909b312ae5813b20a8385374d61614b1c8bd315dcb8df33 |
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| container_issue | 2 |
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| container_title | International journal of food microbiology |
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| creator | Hassan, Abdulwahed Ahmed Akineden, Ömer Kress, Claudia Estuningsih, Sri Schneider, Elisabeth Usleber, Ewald |
| description | The gene encoding the 16S rRNA of
Enterobacter (
E.)
sakazakii (ATCC 29544, plus four strains isolated from powdered infant formula) was studied, and the sequence compared with those of other Enterobacteriaceae in aspects of genetic variability. Sequence differences between
E. sakazakii and other Enterobacteriaceae within the hypervariable regions V1, V2, and V3, respectively, were used to develop two PCR methods for
E. sakazakii. PCR1 employed a primer pair located in V1/V2, while PCR2 utilized a primer pair located in V1/V3, respectively. The two PCR methods were tested against a set of 57
E. sakazakii and 148 non-
E. sakazakii isolates. PCR1 gave an amplicon with a size of 406 bp and resulted in 100% positive results for
E. sakazakii, but also detected
Citrobacter koseri/
amalonaticus and all nine tested
Salmonella enterica serovars. In contrast, PCR2 (amplicon size of 952 bp) gave positive results only for
E. sakazakii, thus allowing specific identification of this species. |
| doi_str_mv | 10.1016/j.ijfoodmicro.2006.12.011 |
| format | article |
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Enterobacter (
E.)
sakazakii (ATCC 29544, plus four strains isolated from powdered infant formula) was studied, and the sequence compared with those of other Enterobacteriaceae in aspects of genetic variability. Sequence differences between
E. sakazakii and other Enterobacteriaceae within the hypervariable regions V1, V2, and V3, respectively, were used to develop two PCR methods for
E. sakazakii. PCR1 employed a primer pair located in V1/V2, while PCR2 utilized a primer pair located in V1/V3, respectively. The two PCR methods were tested against a set of 57
E. sakazakii and 148 non-
E. sakazakii isolates. PCR1 gave an amplicon with a size of 406 bp and resulted in 100% positive results for
E. sakazakii, but also detected
Citrobacter koseri/
amalonaticus and all nine tested
Salmonella enterica serovars. In contrast, PCR2 (amplicon size of 952 bp) gave positive results only for
E. sakazakii, thus allowing specific identification of this species.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2006.12.011</identifier><identifier>PMID: 17289198</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>16S rRNA ; bacterial contamination ; Base Sequence ; Biological and medical sciences ; Citrobacter koseri ; Consumer Product Safety ; Cronobacter sakazakii ; Cronobacter sakazakii - genetics ; Cronobacter sakazakii - isolation & purification ; DNA, Bacterial - chemistry ; Enterobacter ; Enterobacter sakazakii ; Enterobacteriaceae ; food contamination ; Food Contamination - analysis ; Food industries ; Food microbiology ; food pathogens ; Fundamental and applied biological sciences. Psychology ; genes ; Genetic Variation ; Humans ; Infant ; Infant Formula ; infant formulas ; Infant, Newborn ; molecular sequence data ; nucleotide sequences ; pathogen identification ; PCR ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; powdered foods ; Powdered infant formula ; ribosomal RNA ; ribotypes ; RNA, Ribosomal, 16S - genetics ; Salmonella enterica ; Sequence Homology, Nucleic Acid ; Species Specificity ; virulence</subject><ispartof>International journal of food microbiology, 2007-05, Vol.116 (2), p.214-220</ispartof><rights>2007 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-2e8b2676a490187b36909b312ae5813b20a8385374d61614b1c8bd315dcb8df33</citedby><cites>FETCH-LOGICAL-c460t-2e8b2676a490187b36909b312ae5813b20a8385374d61614b1c8bd315dcb8df33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18691977$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17289198$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hassan, Abdulwahed Ahmed</creatorcontrib><creatorcontrib>Akineden, Ömer</creatorcontrib><creatorcontrib>Kress, Claudia</creatorcontrib><creatorcontrib>Estuningsih, Sri</creatorcontrib><creatorcontrib>Schneider, Elisabeth</creatorcontrib><creatorcontrib>Usleber, Ewald</creatorcontrib><title>Characterization of the gene encoding the 16S rRNA of Enterobacter sakazakii and development of a species-specific PCR method</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>The gene encoding the 16S rRNA of
Enterobacter (
E.)
sakazakii (ATCC 29544, plus four strains isolated from powdered infant formula) was studied, and the sequence compared with those of other Enterobacteriaceae in aspects of genetic variability. Sequence differences between
E. sakazakii and other Enterobacteriaceae within the hypervariable regions V1, V2, and V3, respectively, were used to develop two PCR methods for
E. sakazakii. PCR1 employed a primer pair located in V1/V2, while PCR2 utilized a primer pair located in V1/V3, respectively. The two PCR methods were tested against a set of 57
E. sakazakii and 148 non-
E. sakazakii isolates. PCR1 gave an amplicon with a size of 406 bp and resulted in 100% positive results for
E. sakazakii, but also detected
Citrobacter koseri/
amalonaticus and all nine tested
Salmonella enterica serovars. In contrast, PCR2 (amplicon size of 952 bp) gave positive results only for
E. sakazakii, thus allowing specific identification of this species.</description><subject>16S rRNA</subject><subject>bacterial contamination</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Citrobacter koseri</subject><subject>Consumer Product Safety</subject><subject>Cronobacter sakazakii</subject><subject>Cronobacter sakazakii - genetics</subject><subject>Cronobacter sakazakii - isolation & purification</subject><subject>DNA, Bacterial - chemistry</subject><subject>Enterobacter</subject><subject>Enterobacter sakazakii</subject><subject>Enterobacteriaceae</subject><subject>food contamination</subject><subject>Food Contamination - analysis</subject><subject>Food industries</subject><subject>Food microbiology</subject><subject>food pathogens</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genetic Variation</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant Formula</subject><subject>infant formulas</subject><subject>Infant, Newborn</subject><subject>molecular sequence data</subject><subject>nucleotide sequences</subject><subject>pathogen identification</subject><subject>PCR</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>powdered foods</subject><subject>Powdered infant formula</subject><subject>ribosomal RNA</subject><subject>ribotypes</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Salmonella enterica</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Species Specificity</subject><subject>virulence</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqNkEtvEzEURi0EoqHwF8Asym4GPzK2Z1mNykOqALV0bXnsO4nTjB3sSSUq8d_xJJHKktWVrs53Hweh95TUlFDxcVP7zRCjG71NsWaEiJqymlD6DC2okm3Fl4I8R4vCqooK0pyhVzlvCCEN5-QlOqOSqZa2aoH-dGuTjJ0g-Ucz-RhwHPC0BryCABiCjc6H1aFDxS1ON98uZ-IqlETsD0Gczb15NPfeYxMcdvAA27gbIUwzaXDegfWQq0MdvMU_uhs8wrSO7jV6MZhthjeneo7uPl397L5U198_f-0urytbHpkqBqpnQgqzbEn5r-eiJW3PKTPQKMp7RoziquFy6QQVdNlTq3rHaeNsr9zA-Tn6cJy7S_HXHvKkR58tbLcmQNxnXVRI2UhWwPYIFrE5Jxj0LvnRpN-aEj271xv9j3s9u9eU6eK-ZN-eluz7EdxT8iS7ABcnwGRrtkMywfr8xClRMCkL9-7IDSZqs0qFubtlhHJCpBBtM6_qjgQUaQ8eks5FcbDgfAI7aRf9fxz8F3ossV8</recordid><startdate>20070510</startdate><enddate>20070510</enddate><creator>Hassan, Abdulwahed Ahmed</creator><creator>Akineden, Ömer</creator><creator>Kress, Claudia</creator><creator>Estuningsih, Sri</creator><creator>Schneider, Elisabeth</creator><creator>Usleber, Ewald</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20070510</creationdate><title>Characterization of the gene encoding the 16S rRNA of Enterobacter sakazakii and development of a species-specific PCR method</title><author>Hassan, Abdulwahed Ahmed ; Akineden, Ömer ; Kress, Claudia ; Estuningsih, Sri ; Schneider, Elisabeth ; Usleber, Ewald</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-2e8b2676a490187b36909b312ae5813b20a8385374d61614b1c8bd315dcb8df33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>16S rRNA</topic><topic>bacterial contamination</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Citrobacter koseri</topic><topic>Consumer Product Safety</topic><topic>Cronobacter sakazakii</topic><topic>Cronobacter sakazakii - genetics</topic><topic>Cronobacter sakazakii - isolation & purification</topic><topic>DNA, Bacterial - chemistry</topic><topic>Enterobacter</topic><topic>Enterobacter sakazakii</topic><topic>Enterobacteriaceae</topic><topic>food contamination</topic><topic>Food Contamination - analysis</topic><topic>Food industries</topic><topic>Food microbiology</topic><topic>food pathogens</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Genetic Variation</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant Formula</topic><topic>infant formulas</topic><topic>Infant, Newborn</topic><topic>molecular sequence data</topic><topic>nucleotide sequences</topic><topic>pathogen identification</topic><topic>PCR</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>powdered foods</topic><topic>Powdered infant formula</topic><topic>ribosomal RNA</topic><topic>ribotypes</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Salmonella enterica</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Species Specificity</topic><topic>virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hassan, Abdulwahed Ahmed</creatorcontrib><creatorcontrib>Akineden, Ömer</creatorcontrib><creatorcontrib>Kress, Claudia</creatorcontrib><creatorcontrib>Estuningsih, Sri</creatorcontrib><creatorcontrib>Schneider, Elisabeth</creatorcontrib><creatorcontrib>Usleber, Ewald</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hassan, Abdulwahed Ahmed</au><au>Akineden, Ömer</au><au>Kress, Claudia</au><au>Estuningsih, Sri</au><au>Schneider, Elisabeth</au><au>Usleber, Ewald</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the gene encoding the 16S rRNA of Enterobacter sakazakii and development of a species-specific PCR method</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2007-05-10</date><risdate>2007</risdate><volume>116</volume><issue>2</issue><spage>214</spage><epage>220</epage><pages>214-220</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><notes>http://dx.doi.org/10.1016/j.ijfoodmicro.2006.12.011</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>The gene encoding the 16S rRNA of
Enterobacter (
E.)
sakazakii (ATCC 29544, plus four strains isolated from powdered infant formula) was studied, and the sequence compared with those of other Enterobacteriaceae in aspects of genetic variability. Sequence differences between
E. sakazakii and other Enterobacteriaceae within the hypervariable regions V1, V2, and V3, respectively, were used to develop two PCR methods for
E. sakazakii. PCR1 employed a primer pair located in V1/V2, while PCR2 utilized a primer pair located in V1/V3, respectively. The two PCR methods were tested against a set of 57
E. sakazakii and 148 non-
E. sakazakii isolates. PCR1 gave an amplicon with a size of 406 bp and resulted in 100% positive results for
E. sakazakii, but also detected
Citrobacter koseri/
amalonaticus and all nine tested
Salmonella enterica serovars. In contrast, PCR2 (amplicon size of 952 bp) gave positive results only for
E. sakazakii, thus allowing specific identification of this species.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17289198</pmid><doi>10.1016/j.ijfoodmicro.2006.12.011</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1605 |
| ispartof | International journal of food microbiology, 2007-05, Vol.116 (2), p.214-220 |
| issn | 0168-1605 1879-3460 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19877572 |
| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | 16S rRNA bacterial contamination Base Sequence Biological and medical sciences Citrobacter koseri Consumer Product Safety Cronobacter sakazakii Cronobacter sakazakii - genetics Cronobacter sakazakii - isolation & purification DNA, Bacterial - chemistry Enterobacter Enterobacter sakazakii Enterobacteriaceae food contamination Food Contamination - analysis Food industries Food microbiology food pathogens Fundamental and applied biological sciences. Psychology genes Genetic Variation Humans Infant Infant Formula infant formulas Infant, Newborn molecular sequence data nucleotide sequences pathogen identification PCR polymerase chain reaction Polymerase Chain Reaction - methods powdered foods Powdered infant formula ribosomal RNA ribotypes RNA, Ribosomal, 16S - genetics Salmonella enterica Sequence Homology, Nucleic Acid Species Specificity virulence |
| title | Characterization of the gene encoding the 16S rRNA of Enterobacter sakazakii and development of a species-specific PCR method |
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