Biodegradation of methyl parathion and p-nitrophenol: evidence for the presence of a p-nitrophenol 2-hydroxylase in a Gram-negative Serratia sp. strain DS001

A soil bacterium capable of utilizing methyl parathion as sole carbon and energy source was isolated by selective enrichment on minimal medium containing methyl parathion. The strain was identified as belonging to the genus Serratia based on a phylogram constructed using the complete sequence of the...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2007, Vol.73 (6), p.1452-1462
Main Authors: Pakala, Suresh B, Gorla, Purushotham, Pinjari, Aleem Basha, Krovidi, Ravi Kumar, Baru, Rajasekhar, Yanamandra, Mahesh, Merrick, Mike, Siddavattam, Dayananda
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Proteins - metabolism
Biodegradation
Biodegradation, Environmental
Biological and medical sciences
Biotechnology
Carbon
Catabolomics
Cytochrome P-450 CYP2E1 - metabolism
Electrophoresis, Gel, Two-Dimensional
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
Gram-positive bacteria
Methyl Parathion - chemistry
Methyl Parathion - metabolism
Microbiology
Models, Chemical
Molecular Sequence Data
Molecular Structure
Nitrophenols - chemistry
Nitrophenols - metabolism
Oxygen - metabolism
p-Nitrophenol hydroxylase component A
Parathion hydrolase
Phylogeny
Serratia
Serratia - classification
Serratia - genetics
Serratia - metabolism
Serratia sp
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:A soil bacterium capable of utilizing methyl parathion as sole carbon and energy source was isolated by selective enrichment on minimal medium containing methyl parathion. The strain was identified as belonging to the genus Serratia based on a phylogram constructed using the complete sequence of the 16S rRNA. Serratia sp. strain DS001 utilized methyl parathion, p-nitrophenol, 4-nitrocatechol, and 1,2,4-benzenetriol as sole carbon and energy sources but could not grow using hydroquinone as a source of carbon. p-Nitrophenol and dimethylthiophosphoric acid were found to be the major degradation products of methyl parathion. Growth on p-nitrophenol led to release of stoichiometric amounts of nitrite and to the formation of 4-nitrocatechol and benzenetriol. When these catabolic intermediates of p-nitrophenol were added to resting cells of Serratia sp. strain DS001 oxygen consumption was detected whereas no oxygen consumption was apparent when hydroquinone was added to the resting cells suggesting that it is not part of the p-nitrophenol degradation pathway. Key enzymes involved in degradation of methyl parathion and in conversion of p-nitrophenol to 4-nitrocatechol, namely parathion hydrolase and p-nitrophenol hydroxylase component “A” were detected in the proteomes of the methyl parathion and p-nitrophenol grown cultures, respectively. These studies report for the first time the existence of a p-nitrophenol hydroxylase component “A”, typically found in Gram-positive bacteria, in a Gram-negative strain of the genus Serratia.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-006-0595-z