Effect of T- and C-loop mutations on the Herbaspirillum seropedicae GlnB protein in nitrogen signalling

Proteins of the PII family are found in species of all kingdoms. Although these proteins usually share high identity, their functions are specific to the different organisms. Comparison of structural data from Escherichia coli GlnB and GlnK and Herbaspirillum seropedicae GlnB showed that the T-loop...

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Bibliographic Details
Published in:Research in microbiology 2005-06, Vol.156 (5), p.634-640
Main Authors: Bonatto, Ana C., Souza, Emanuel M., Pedrosa, Fábio O., Yates, M. Geoffrey, Benelli, Elaine M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acid Substitution
Artificial Gene Fusion
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
beta-Galactosidase - analysis
Biological and medical sciences
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Genes, Reporter
GlnB protein
Herbaspirillum - genetics
Herbaspirillum - metabolism
Herbaspirillum seropedicae
Klebsiella pneumoniae
Microbiology
Miscellaneous
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
NifA activity
Nitrogen - metabolism
PII Nitrogen Regulatory Proteins
Protein Structure, Secondary
Sequence Homology, Amino Acid
Signal Transduction
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Summary:Proteins of the PII family are found in species of all kingdoms. Although these proteins usually share high identity, their functions are specific to the different organisms. Comparison of structural data from Escherichia coli GlnB and GlnK and Herbaspirillum seropedicae GlnB showed that the T-loop and C-terminus were variable regions. To evaluate the role of these regions in signal transduction by the H. seropedicae GlnB protein, four mutants were constructed: Y51F, G108A/P109a, G108W and Q3R/T5A. The activities of the native and mutated proteins were assayed in an E. coli background constitutively expressing the Klebsiella pneumoniae nifLA operon. The results suggested that the T-loop and C-terminus regions of H. seropedicae GlnB are involved in nitrogen signal transduction.
ISSN:0923-2508
1769-7123
DOI:10.1016/j.resmic.2005.01.015