Knockdown of eukaryotic translation initiation factor 3 subunit D (eIF3D) inhibits proliferation of acute myeloid leukemia cells

Various eukaryotic translation initiation factors (eIFs) have been implicated in carcinoma development. Eukaryotic translation initiation factor 3 subunit D (eIF3D) has recently been shown to regulate the growth of several types of human cancer cells. However, the function of eIF3D in acute myeloid...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 2018-01, Vol.438 (1-2), p.191-198
Main Authors: Liu, Guo-zhen, Liu, Ji-zhu, Li, Xiao-qing, Zhang, Li, Li, Shuang-jing, Xiao, Tai-wu, Wang, Jing-xia, Li, Guang-yao, Liu, Yusen
Format: Article
Language:English
Subjects:
Acute myelocytic leukemia
Analysis
Biochemistry
Biomedical and Life Sciences
Cancer research
Cardiology
Cell Proliferation
Eukaryotic Initiation Factor-3 - genetics
Eukaryotic Initiation Factor-3 - metabolism
Gene Knockdown Techniques
Health aspects
Humans
Leukemia, Myeloid, Acute - genetics
Leukemia, Myeloid, Acute - metabolism
Leukemia, Myeloid, Acute - pathology
Life Sciences
Medical Biochemistry
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism
Oncology
RNA
Translation (Genetics)
U937 Cells
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Summary:Various eukaryotic translation initiation factors (eIFs) have been implicated in carcinoma development. Eukaryotic translation initiation factor 3 subunit D (eIF3D) has recently been shown to regulate the growth of several types of human cancer cells. However, the function of eIF3D in acute myeloid leukemia (AML) remains unclear. In this study, we investigated the expression of eIF3D in three AML cell lines and a lymphoblast cell line, and found that eIF3D was expressed in all four leukemia cell lines. To explore the role of eIF3D in AML cell proliferation, lentivirus-mediated RNA interference was applied to knock down the expression of eIF3D in U937 cells. The expression of eIF3D was significantly downregulated in U937 cells after eIF3D knockdown, as confirmed by quantitative real-time PCR (qRT-PCR) and Western blot analysis. Knockdown of eIF3D significantly inhibited proliferation of U937 cells. Furthermore, flow cytometry analysis revealed that eIF3D silencing induced cell cycle arrest at the G2/M phase, ultimately leading to apoptosis. Our results indicate that eIF3D plays a key role in the proliferation of AML cells, and suggest that eIF3D silencing might be a potential therapeutic strategy for leukemia.
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-017-3127-5