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Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion
Targeted editing of single base pairs is achieved in monocot rice and dicot tomato using Target-AID (Cas9 activation-induced cytidine deaminase fusion). We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by sin...
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| Published in: | Nature biotechnology 2017-05, Vol.35 (5), p.441-443 |
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| Main Authors: | , , , , , , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c616t-91a11f86ac1cf4bb1e587066f6e32cce6e1b17795fc7d052b3289ca0a27bcbf53 |
| container_end_page | 443 |
| container_issue | 5 |
| container_start_page | 441 |
| container_title | Nature biotechnology |
| container_volume | 35 |
| creator | Shimatani, Zenpei Kashojiya, Sachiko Takayama, Mariko Terada, Rie Arazoe, Takayuki Ishii, Hisaki Teramura, Hiroshi Yamamoto, Tsuyoshi Komatsu, Hiroki Miura, Kenji Ezura, Hiroshi Nishida, Keiji Ariizumi, Tohru Kondo, Akihiko |
| description | Targeted editing of single base pairs is achieved in monocot rice and dicot tomato using Target-AID (Cas9 activation-induced cytidine deaminase fusion).
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement. |
| doi_str_mv | 10.1038/nbt.3833 |
| format | article |
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We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.</description><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt.3833</identifier><identifier>PMID: 28346401</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>45/41 ; 45/70 ; 631/449/711 ; 631/553/2690 ; Agriculture ; Base Pairing - genetics ; Bioinformatics ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; brief-communication ; Cereal crops ; Clustered Regularly Interspaced Short Palindromic Repeats - genetics ; CRISPR-Associated Proteins - genetics ; Crop improvement ; Crops ; Cytidine Deaminase - genetics ; Deoxyribonucleic acid ; DNA ; DNA, Plant - genetics ; Enzymes ; Gene Editing - methods ; Genes, Plant - genetics ; Genetic aspects ; Genetic engineering ; Herbicides ; Life Sciences ; Lycopersicon esculentum ; Lycopersicon esculentum - genetics ; Methods ; Mutagenesis ; Mutagenesis, Site-Directed - methods ; Mutation ; Oryza - genetics ; Plant genetic engineering ; Plants, Genetically Modified - genetics ; Point Mutation - genetics ; Recombinant Fusion Proteins - genetics ; Ribonucleic acid ; Rice ; RNA ; Tomatoes</subject><ispartof>Nature biotechnology, 2017-05, Vol.35 (5), p.441-443</ispartof><rights>Springer Nature America, Inc. 2017</rights><rights>COPYRIGHT 2017 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group May 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c616t-91a11f86ac1cf4bb1e587066f6e32cce6e1b17795fc7d052b3289ca0a27bcbf53</citedby><cites>FETCH-LOGICAL-c616t-91a11f86ac1cf4bb1e587066f6e32cce6e1b17795fc7d052b3289ca0a27bcbf53</cites><orcidid>0000-0002-9371-4971 ; 0000-0003-1527-5288</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,27936,27937</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28346401$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shimatani, Zenpei</creatorcontrib><creatorcontrib>Kashojiya, Sachiko</creatorcontrib><creatorcontrib>Takayama, Mariko</creatorcontrib><creatorcontrib>Terada, Rie</creatorcontrib><creatorcontrib>Arazoe, Takayuki</creatorcontrib><creatorcontrib>Ishii, Hisaki</creatorcontrib><creatorcontrib>Teramura, Hiroshi</creatorcontrib><creatorcontrib>Yamamoto, Tsuyoshi</creatorcontrib><creatorcontrib>Komatsu, Hiroki</creatorcontrib><creatorcontrib>Miura, Kenji</creatorcontrib><creatorcontrib>Ezura, Hiroshi</creatorcontrib><creatorcontrib>Nishida, Keiji</creatorcontrib><creatorcontrib>Ariizumi, Tohru</creatorcontrib><creatorcontrib>Kondo, Akihiko</creatorcontrib><title>Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion</title><title>Nature biotechnology</title><addtitle>Nat Biotechnol</addtitle><addtitle>Nat Biotechnol</addtitle><description>Targeted editing of single base pairs is achieved in monocot rice and dicot tomato using Target-AID (Cas9 activation-induced cytidine deaminase fusion).
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.</description><subject>45/41</subject><subject>45/70</subject><subject>631/449/711</subject><subject>631/553/2690</subject><subject>Agriculture</subject><subject>Base Pairing - genetics</subject><subject>Bioinformatics</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>brief-communication</subject><subject>Cereal crops</subject><subject>Clustered Regularly Interspaced Short Palindromic Repeats - genetics</subject><subject>CRISPR-Associated Proteins - genetics</subject><subject>Crop improvement</subject><subject>Crops</subject><subject>Cytidine Deaminase - genetics</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Plant - genetics</subject><subject>Enzymes</subject><subject>Gene Editing - methods</subject><subject>Genes, Plant - genetics</subject><subject>Genetic aspects</subject><subject>Genetic engineering</subject><subject>Herbicides</subject><subject>Life Sciences</subject><subject>Lycopersicon esculentum</subject><subject>Lycopersicon esculentum - genetics</subject><subject>Methods</subject><subject>Mutagenesis</subject><subject>Mutagenesis, Site-Directed - methods</subject><subject>Mutation</subject><subject>Oryza - genetics</subject><subject>Plant genetic engineering</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Point Mutation - genetics</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Ribonucleic 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Akihiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion</atitle><jtitle>Nature biotechnology</jtitle><stitle>Nat Biotechnol</stitle><addtitle>Nat Biotechnol</addtitle><date>2017-05-01</date><risdate>2017</risdate><volume>35</volume><issue>5</issue><spage>441</spage><epage>443</epage><pages>441-443</pages><issn>1087-0156</issn><eissn>1546-1696</eissn><abstract>Targeted editing of single base pairs is achieved in monocot rice and dicot tomato using Target-AID (Cas9 activation-induced cytidine deaminase fusion).
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>28346401</pmid><doi>10.1038/nbt.3833</doi><tpages>3</tpages><orcidid>https://orcid.org/0000-0002-9371-4971</orcidid><orcidid>https://orcid.org/0000-0003-1527-5288</orcidid></addata></record> |
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| ispartof | Nature biotechnology, 2017-05, Vol.35 (5), p.441-443 |
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| source | Nature |
| subjects | 45/41 45/70 631/449/711 631/553/2690 Agriculture Base Pairing - genetics Bioinformatics Biomedical Engineering/Biotechnology Biomedicine Biotechnology brief-communication Cereal crops Clustered Regularly Interspaced Short Palindromic Repeats - genetics CRISPR-Associated Proteins - genetics Crop improvement Crops Cytidine Deaminase - genetics Deoxyribonucleic acid DNA DNA, Plant - genetics Enzymes Gene Editing - methods Genes, Plant - genetics Genetic aspects Genetic engineering Herbicides Life Sciences Lycopersicon esculentum Lycopersicon esculentum - genetics Methods Mutagenesis Mutagenesis, Site-Directed - methods Mutation Oryza - genetics Plant genetic engineering Plants, Genetically Modified - genetics Point Mutation - genetics Recombinant Fusion Proteins - genetics Ribonucleic acid Rice RNA Tomatoes |
| title | Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion |
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