Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide sele...

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Bibliographic Details
Published in:Nature biotechnology 2017-05, Vol.35 (5), p.441-443
Main Authors: Shimatani, Zenpei, Kashojiya, Sachiko, Takayama, Mariko, Terada, Rie, Arazoe, Takayuki, Ishii, Hisaki, Teramura, Hiroshi, Yamamoto, Tsuyoshi, Komatsu, Hiroki, Miura, Kenji, Ezura, Hiroshi, Nishida, Keiji, Ariizumi, Tohru, Kondo, Akihiko
Format: Article
Language:English
Subjects:
Base Pairing - genetics
Clustered Regularly Interspaced Short Palindromic Repeats - genetics
CRISPR-Associated Proteins - genetics
Crops
Cytidine Deaminase - genetics
DNA, Plant - genetics
Enzymes
Gene Editing - methods
Genes, Plant - genetics
Genetic aspects
Genetic engineering
Lycopersicon esculentum
Lycopersicon esculentum - genetics
Methods
Mutagenesis
Mutagenesis, Site-Directed - methods
Mutation
Oryza - genetics
Plant genetic engineering
Plants, Genetically Modified - genetics
Point Mutation - genetics
Recombinant Fusion Proteins - genetics
Ribonucleic acid
Rice
RNA
Tomatoes
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Summary:We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt.3833