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Analysis of the Vibrio pathogenicity island-encoded Mop protein suggests a pleiotropic role in the virulence of epidemic Vibrio cholerae
Epidemic Vibrio cholerae contain a large essential virulence gene cluster called the Vibrio pathogenicity island (VPI). We recently reported that no in vitro difference in virulence was found in El Tor strain N16961 containing a mutation in the VPI-encoded mop gene but this mutant was hypervirulent...
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| Published in: | FEMS microbiology letters 2003-08, Vol.225 (2), p.311-318 |
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| container_issue | 2 |
| container_start_page | 311 |
| container_title | FEMS microbiology letters |
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| creator | Zhang, Dalin Rajanna, Chythanya Sun, Weiyun Karaolis, David K.R. |
| description | Epidemic
Vibrio cholerae contain a large essential virulence gene cluster called the
Vibrio pathogenicity island (VPI). We recently reported that no in vitro difference in virulence was found in El Tor strain N16961 containing a mutation in the VPI-encoded
mop gene but this mutant was hypervirulent and reactogenic in rabbit ileal loops. In this paper, we report in vitro studies showing that independent Mop mutants of strain 3083 are significantly attenuated (∼40-fold) in cholera toxin (CT) production and have significantly increased motility and biofilm forming ability but appear to be unaffected in TcpA, hemagglutinin protease and hemolysin compared to their parent. The 3083 Mop mutant showed a 100-fold decrease in its in vivo intestinal colonization ability in the infant mouse competition assays. While reverse transcription polymerase chain reaction and phenotypic studies of a
mop plasmid in both mutant and wild-type backgrounds suggest Mop is expressed by the plasmid, the differences in CT and biofilm formation could not be restored in any of the mutants. The inability to complement the Mop mutants
in trans may be due either to the selection of secondary mutations or to
mop possibly being part of an operon. Our findings that Mop is associated with CT, motility, biofilm formation and intestinal colonization support a hypothesis in which Mop has a pleiotropic role in the pathogenesis and persistence of epidemic
V. cholerae. |
| doi_str_mv | 10.1016/S0378-1097(03)00535-4 |
| format | article |
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Vibrio cholerae contain a large essential virulence gene cluster called the
Vibrio pathogenicity island (VPI). We recently reported that no in vitro difference in virulence was found in El Tor strain N16961 containing a mutation in the VPI-encoded
mop gene but this mutant was hypervirulent and reactogenic in rabbit ileal loops. In this paper, we report in vitro studies showing that independent Mop mutants of strain 3083 are significantly attenuated (∼40-fold) in cholera toxin (CT) production and have significantly increased motility and biofilm forming ability but appear to be unaffected in TcpA, hemagglutinin protease and hemolysin compared to their parent. The 3083 Mop mutant showed a 100-fold decrease in its in vivo intestinal colonization ability in the infant mouse competition assays. While reverse transcription polymerase chain reaction and phenotypic studies of a
mop plasmid in both mutant and wild-type backgrounds suggest Mop is expressed by the plasmid, the differences in CT and biofilm formation could not be restored in any of the mutants. The inability to complement the Mop mutants
in trans may be due either to the selection of secondary mutations or to
mop possibly being part of an operon. Our findings that Mop is associated with CT, motility, biofilm formation and intestinal colonization support a hypothesis in which Mop has a pleiotropic role in the pathogenesis and persistence of epidemic
V. cholerae.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/S0378-1097(03)00535-4</identifier><identifier>PMID: 12951258</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Animals ; Bacteriology ; Biofilm ; Biofilms - growth & development ; Biological and medical sciences ; Blotting, Western ; Cell Adhesion - genetics ; Cholera ; Cholera Toxin - biosynthesis ; Colonization ; Fimbriae Proteins - biosynthesis ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genetic Complementation Test ; Genetics ; Hemolysin Proteins - biosynthesis ; Intestines - microbiology ; Metalloendopeptidases - biosynthesis ; Metalloendopeptidases - genetics ; Metalloendopeptidases - physiology ; Mice ; Microbiology ; Mop ; Movement ; Mutation ; Pathogenesis ; Pathogenicity island ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Reverse Transcriptase Polymerase Chain Reaction ; Vibrio cholerae ; Vibrio cholerae - genetics ; Vibrio cholerae - pathogenicity ; Vibrio cholerae - physiology ; Virulence ; Virulence - genetics ; Virulence Factors - genetics ; Virulence Factors - metabolism</subject><ispartof>FEMS microbiology letters, 2003-08, Vol.225 (2), p.311-318</ispartof><rights>2003</rights><rights>2003 Federation of European Microbiological Societies 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5131-8d2c8569c2729f87842e14c86043137363b8198f8dda04e17dc8c72c72ad43bd3</citedby><cites>FETCH-LOGICAL-c5131-8d2c8569c2729f87842e14c86043137363b8198f8dda04e17dc8c72c72ad43bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15244584$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12951258$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Dalin</creatorcontrib><creatorcontrib>Rajanna, Chythanya</creatorcontrib><creatorcontrib>Sun, Weiyun</creatorcontrib><creatorcontrib>Karaolis, David K.R.</creatorcontrib><title>Analysis of the Vibrio pathogenicity island-encoded Mop protein suggests a pleiotropic role in the virulence of epidemic Vibrio cholerae</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Epidemic
Vibrio cholerae contain a large essential virulence gene cluster called the
Vibrio pathogenicity island (VPI). We recently reported that no in vitro difference in virulence was found in El Tor strain N16961 containing a mutation in the VPI-encoded
mop gene but this mutant was hypervirulent and reactogenic in rabbit ileal loops. In this paper, we report in vitro studies showing that independent Mop mutants of strain 3083 are significantly attenuated (∼40-fold) in cholera toxin (CT) production and have significantly increased motility and biofilm forming ability but appear to be unaffected in TcpA, hemagglutinin protease and hemolysin compared to their parent. The 3083 Mop mutant showed a 100-fold decrease in its in vivo intestinal colonization ability in the infant mouse competition assays. While reverse transcription polymerase chain reaction and phenotypic studies of a
mop plasmid in both mutant and wild-type backgrounds suggest Mop is expressed by the plasmid, the differences in CT and biofilm formation could not be restored in any of the mutants. The inability to complement the Mop mutants
in trans may be due either to the selection of secondary mutations or to
mop possibly being part of an operon. Our findings that Mop is associated with CT, motility, biofilm formation and intestinal colonization support a hypothesis in which Mop has a pleiotropic role in the pathogenesis and persistence of epidemic
V. cholerae.</description><subject>Animals</subject><subject>Bacteriology</subject><subject>Biofilm</subject><subject>Biofilms - growth & development</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Adhesion - genetics</subject><subject>Cholera</subject><subject>Cholera Toxin - biosynthesis</subject><subject>Colonization</subject><subject>Fimbriae Proteins - biosynthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genetic Complementation Test</subject><subject>Genetics</subject><subject>Hemolysin Proteins - biosynthesis</subject><subject>Intestines - microbiology</subject><subject>Metalloendopeptidases - biosynthesis</subject><subject>Metalloendopeptidases - genetics</subject><subject>Metalloendopeptidases - physiology</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Mop</subject><subject>Movement</subject><subject>Mutation</subject><subject>Pathogenesis</subject><subject>Pathogenicity island</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Vibrio cholerae</subject><subject>Vibrio cholerae - genetics</subject><subject>Vibrio cholerae - pathogenicity</subject><subject>Vibrio cholerae - physiology</subject><subject>Virulence</subject><subject>Virulence - genetics</subject><subject>Virulence Factors - genetics</subject><subject>Virulence Factors - metabolism</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqNkctu1DAUhi0EotPCI4C8AcEixdfEWVVVRSnSVCy4bC2PfTJjlImDnRTNG_Sx6zQRFRISlSx54e87v885CL2i5JQSWn74SnilCkrq6h3h7wmRXBbiCVpRWYmirEv1FK3-IEfoOKWfhBDBSPkcHVFWS8qkWqHb8860h-QTDg0edoB_-E30Afdm2IUtdN764YB9ak3nCuhscODwdehxH8MAvsNp3G4hDQkb3LfgwxBD7y2OoQWcn6eSNz6ObXZhyoDeO9hnYgmyu0xGAy_Qs8a0CV4u9wn6fvnx28VVsf7y6fPF-bqwknJaKMeskmVtWcXqRlVKMKDCqpIITnnFS75RtFaNcs4QAbRyVtmK5WOc4BvHT9DbuW5u4NeYf673Plloc4MQxqSpUpVQrMygnEEbQ0oRGt1HvzfxoCnR0wr0_Qr0NF9NuL5fgRbZe70EjJs9uAdrmXkG3iyASda0TTSd9emBk0wIqaZC9cz99i0cHpeuL6_XnNLsktkNY_9vs_jLLKa4s1mBPP0bD1En66etOR_BDtoF_5_O7wD1OMLN</recordid><startdate>20030829</startdate><enddate>20030829</enddate><creator>Zhang, Dalin</creator><creator>Rajanna, Chythanya</creator><creator>Sun, Weiyun</creator><creator>Karaolis, David K.R.</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20030829</creationdate><title>Analysis of the Vibrio pathogenicity island-encoded Mop protein suggests a pleiotropic role in the virulence of epidemic Vibrio cholerae</title><author>Zhang, Dalin ; Rajanna, Chythanya ; Sun, Weiyun ; Karaolis, David K.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5131-8d2c8569c2729f87842e14c86043137363b8198f8dda04e17dc8c72c72ad43bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Bacteriology</topic><topic>Biofilm</topic><topic>Biofilms - growth & development</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cell Adhesion - genetics</topic><topic>Cholera</topic><topic>Cholera Toxin - biosynthesis</topic><topic>Colonization</topic><topic>Fimbriae Proteins - biosynthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genetic Complementation Test</topic><topic>Genetics</topic><topic>Hemolysin Proteins - biosynthesis</topic><topic>Intestines - microbiology</topic><topic>Metalloendopeptidases - biosynthesis</topic><topic>Metalloendopeptidases - genetics</topic><topic>Metalloendopeptidases - physiology</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Mop</topic><topic>Movement</topic><topic>Mutation</topic><topic>Pathogenesis</topic><topic>Pathogenicity island</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Vibrio cholerae</topic><topic>Vibrio cholerae - genetics</topic><topic>Vibrio cholerae - pathogenicity</topic><topic>Vibrio cholerae - physiology</topic><topic>Virulence</topic><topic>Virulence - genetics</topic><topic>Virulence Factors - genetics</topic><topic>Virulence Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Dalin</creatorcontrib><creatorcontrib>Rajanna, Chythanya</creatorcontrib><creatorcontrib>Sun, Weiyun</creatorcontrib><creatorcontrib>Karaolis, David K.R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Dalin</au><au>Rajanna, Chythanya</au><au>Sun, Weiyun</au><au>Karaolis, David K.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of the Vibrio pathogenicity island-encoded Mop protein suggests a pleiotropic role in the virulence of epidemic Vibrio cholerae</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2003-08-29</date><risdate>2003</risdate><volume>225</volume><issue>2</issue><spage>311</spage><epage>318</epage><pages>311-318</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><abstract>Epidemic
Vibrio cholerae contain a large essential virulence gene cluster called the
Vibrio pathogenicity island (VPI). We recently reported that no in vitro difference in virulence was found in El Tor strain N16961 containing a mutation in the VPI-encoded
mop gene but this mutant was hypervirulent and reactogenic in rabbit ileal loops. In this paper, we report in vitro studies showing that independent Mop mutants of strain 3083 are significantly attenuated (∼40-fold) in cholera toxin (CT) production and have significantly increased motility and biofilm forming ability but appear to be unaffected in TcpA, hemagglutinin protease and hemolysin compared to their parent. The 3083 Mop mutant showed a 100-fold decrease in its in vivo intestinal colonization ability in the infant mouse competition assays. While reverse transcription polymerase chain reaction and phenotypic studies of a
mop plasmid in both mutant and wild-type backgrounds suggest Mop is expressed by the plasmid, the differences in CT and biofilm formation could not be restored in any of the mutants. The inability to complement the Mop mutants
in trans may be due either to the selection of secondary mutations or to
mop possibly being part of an operon. Our findings that Mop is associated with CT, motility, biofilm formation and intestinal colonization support a hypothesis in which Mop has a pleiotropic role in the pathogenesis and persistence of epidemic
V. cholerae.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>12951258</pmid><doi>10.1016/S0378-1097(03)00535-4</doi><tpages>8</tpages></addata></record> |
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| ispartof | FEMS microbiology letters, 2003-08, Vol.225 (2), p.311-318 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | Oxford Journals |
| subjects | Animals Bacteriology Biofilm Biofilms - growth & development Biological and medical sciences Blotting, Western Cell Adhesion - genetics Cholera Cholera Toxin - biosynthesis Colonization Fimbriae Proteins - biosynthesis Fundamental and applied biological sciences. Psychology Gene Expression Genetic Complementation Test Genetics Hemolysin Proteins - biosynthesis Intestines - microbiology Metalloendopeptidases - biosynthesis Metalloendopeptidases - genetics Metalloendopeptidases - physiology Mice Microbiology Mop Movement Mutation Pathogenesis Pathogenicity island Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Reverse Transcriptase Polymerase Chain Reaction Vibrio cholerae Vibrio cholerae - genetics Vibrio cholerae - pathogenicity Vibrio cholerae - physiology Virulence Virulence - genetics Virulence Factors - genetics Virulence Factors - metabolism |
| title | Analysis of the Vibrio pathogenicity island-encoded Mop protein suggests a pleiotropic role in the virulence of epidemic Vibrio cholerae |
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