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Environmental and mucosal microbiota and their role in childhood asthma
Background High microbial diversity in the environment has been associated with lower asthma risk, particularly in children exposed to farming. It remains unclear whether this effect operates through an altered microbiome of the mucosal surfaces of the airways. Methods DNA from mattress dust and nas...
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| Published in: | Allergy (Copenhagen) 2017-01, Vol.72 (1), p.109-119 |
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| container_title | Allergy (Copenhagen) |
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| creator | Birzele, L. T. Depner, M. Ege, M. J. Engel, M. Kublik, S. Bernau, C. Loss, G. J. Genuneit, J. Horak, E. Schloter, M. Braun‐Fahrländer, C. Danielewicz, H. Heederik, D. von Mutius, E. Legatzki, A. |
| description | Background
High microbial diversity in the environment has been associated with lower asthma risk, particularly in children exposed to farming. It remains unclear whether this effect operates through an altered microbiome of the mucosal surfaces of the airways.
Methods
DNA from mattress dust and nasal samples of 86 school age children was analyzed by 454 pyrosequencing of the 16S rRNA gene fragments. Based on operational taxonomic units (OTUs), bacterial diversity and composition were related to farm exposure and asthma status.
Results
Farm exposure was positively associated with bacterial diversity in mattress dust samples as determined by richness (P = 8.1 × 10−6) and Shannon index (P = 1.3 × 10−5). Despite considerable agreement of richness between mattress and nasal samples, the association of richness with farming in nasal samples was restricted to a high gradient of farm exposure, that is, exposure to cows and straw vs no exposure at all. In mattress dust, the genera Clostridium, Facklamia, an unclassified genus within the family of Ruminococcaceae, and six OTUs were positively associated with farming. Asthma was inversely associated with richness [aOR = 0.48 (0.22–1.02)] and Shannon index [aOR = 0.41 (0.21–0.83)] in mattress dust and to a lower extent in nasal samples [richness aOR 0.63 = (0.38–1.06), Shannon index aOR = 0.66 (0.39–1.12)].
Conclusion
The stronger inverse association of asthma with bacterial diversity in mattress dust as compared to nasal samples suggests microbial involvement beyond mere colonization of the upper airways. Whether inhalation of metabolites of environmental bacteria contributes to this phenomenon should be the focus of future research. |
| doi_str_mv | 10.1111/all.13002 |
| format | article |
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High microbial diversity in the environment has been associated with lower asthma risk, particularly in children exposed to farming. It remains unclear whether this effect operates through an altered microbiome of the mucosal surfaces of the airways.
Methods
DNA from mattress dust and nasal samples of 86 school age children was analyzed by 454 pyrosequencing of the 16S rRNA gene fragments. Based on operational taxonomic units (OTUs), bacterial diversity and composition were related to farm exposure and asthma status.
Results
Farm exposure was positively associated with bacterial diversity in mattress dust samples as determined by richness (P = 8.1 × 10−6) and Shannon index (P = 1.3 × 10−5). Despite considerable agreement of richness between mattress and nasal samples, the association of richness with farming in nasal samples was restricted to a high gradient of farm exposure, that is, exposure to cows and straw vs no exposure at all. In mattress dust, the genera Clostridium, Facklamia, an unclassified genus within the family of Ruminococcaceae, and six OTUs were positively associated with farming. Asthma was inversely associated with richness [aOR = 0.48 (0.22–1.02)] and Shannon index [aOR = 0.41 (0.21–0.83)] in mattress dust and to a lower extent in nasal samples [richness aOR 0.63 = (0.38–1.06), Shannon index aOR = 0.66 (0.39–1.12)].
Conclusion
The stronger inverse association of asthma with bacterial diversity in mattress dust as compared to nasal samples suggests microbial involvement beyond mere colonization of the upper airways. Whether inhalation of metabolites of environmental bacteria contributes to this phenomenon should be the focus of future research.</description><identifier>ISSN: 0105-4538</identifier><identifier>EISSN: 1398-9995</identifier><identifier>DOI: 10.1111/all.13002</identifier><identifier>PMID: 27503830</identifier><language>eng</language><publisher>Denmark: Blackwell Publishing Ltd</publisher><subject>Asthma ; Asthma - epidemiology ; Asthma - etiology ; Bacteria - classification ; Bacteria - genetics ; Biodiversity ; Child ; Cross-Sectional Studies ; Environmental Exposure - adverse effects ; Environmental health ; Environmental Microbiology ; epidemiology ; Female ; Humans ; Male ; Microbiology ; microbiome ; Microbiota ; Mucous Membrane - microbiology ; pediatrics</subject><ispartof>Allergy (Copenhagen), 2017-01, Vol.72 (1), p.109-119</ispartof><rights>2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4212-9796bdda40f3c15dc8080cf223c66ad0668f1845c1a254dc351652b81a42c1af3</citedby><cites>FETCH-LOGICAL-c4212-9796bdda40f3c15dc8080cf223c66ad0668f1845c1a254dc351652b81a42c1af3</cites><orcidid>0000-0001-5764-1528</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,27936,27937</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27503830$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Birzele, L. T.</creatorcontrib><creatorcontrib>Depner, M.</creatorcontrib><creatorcontrib>Ege, M. J.</creatorcontrib><creatorcontrib>Engel, M.</creatorcontrib><creatorcontrib>Kublik, S.</creatorcontrib><creatorcontrib>Bernau, C.</creatorcontrib><creatorcontrib>Loss, G. J.</creatorcontrib><creatorcontrib>Genuneit, J.</creatorcontrib><creatorcontrib>Horak, E.</creatorcontrib><creatorcontrib>Schloter, M.</creatorcontrib><creatorcontrib>Braun‐Fahrländer, C.</creatorcontrib><creatorcontrib>Danielewicz, H.</creatorcontrib><creatorcontrib>Heederik, D.</creatorcontrib><creatorcontrib>von Mutius, E.</creatorcontrib><creatorcontrib>Legatzki, A.</creatorcontrib><title>Environmental and mucosal microbiota and their role in childhood asthma</title><title>Allergy (Copenhagen)</title><addtitle>Allergy</addtitle><description>Background
High microbial diversity in the environment has been associated with lower asthma risk, particularly in children exposed to farming. It remains unclear whether this effect operates through an altered microbiome of the mucosal surfaces of the airways.
Methods
DNA from mattress dust and nasal samples of 86 school age children was analyzed by 454 pyrosequencing of the 16S rRNA gene fragments. Based on operational taxonomic units (OTUs), bacterial diversity and composition were related to farm exposure and asthma status.
Results
Farm exposure was positively associated with bacterial diversity in mattress dust samples as determined by richness (P = 8.1 × 10−6) and Shannon index (P = 1.3 × 10−5). Despite considerable agreement of richness between mattress and nasal samples, the association of richness with farming in nasal samples was restricted to a high gradient of farm exposure, that is, exposure to cows and straw vs no exposure at all. In mattress dust, the genera Clostridium, Facklamia, an unclassified genus within the family of Ruminococcaceae, and six OTUs were positively associated with farming. Asthma was inversely associated with richness [aOR = 0.48 (0.22–1.02)] and Shannon index [aOR = 0.41 (0.21–0.83)] in mattress dust and to a lower extent in nasal samples [richness aOR 0.63 = (0.38–1.06), Shannon index aOR = 0.66 (0.39–1.12)].
Conclusion
The stronger inverse association of asthma with bacterial diversity in mattress dust as compared to nasal samples suggests microbial involvement beyond mere colonization of the upper airways. Whether inhalation of metabolites of environmental bacteria contributes to this phenomenon should be the focus of future research.</description><subject>Asthma</subject><subject>Asthma - epidemiology</subject><subject>Asthma - etiology</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Biodiversity</subject><subject>Child</subject><subject>Cross-Sectional Studies</subject><subject>Environmental Exposure - adverse effects</subject><subject>Environmental health</subject><subject>Environmental Microbiology</subject><subject>epidemiology</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Microbiology</subject><subject>microbiome</subject><subject>Microbiota</subject><subject>Mucous Membrane - microbiology</subject><subject>pediatrics</subject><issn>0105-4538</issn><issn>1398-9995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp10EFLwzAYBuAgipvTg39ACl700O1L0nTpUcacwsCLnkuapDQjbWbSKvv3xm16EMwl4ePhJd-L0DWGKY5nJqydYgpATtAY04KnRVGwUzQGDCzNGOUjdBHCBgDmpIBzNCJzBpRTGKPVsvsw3nWt7nphE9GppB2kC_HdGuldZVwv9uO-0cYn3lmdmC6RjbGqcU4lIvRNKy7RWS1s0FfHe4LeHpevi6d0_bJ6XjysU5kRTNJiXuSVUiKDmkrMlOTAQdaEUJnnQkGe8xrzjEksCMuUpAznjFQci4zEWU0n6O6Qu_XufdChL1sTpLZWdNoNocScsbglwzzS2z904wbfxd9FlXGAvJjjqO4PKi4bgtd1ufWmFX5XYii_2y1ju-W-3WhvjolD1Wr1K3_qjGB2AJ_G6t3_SeXDen2I_ALghoHQ</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Birzele, L. T.</creator><creator>Depner, M.</creator><creator>Ege, M. J.</creator><creator>Engel, M.</creator><creator>Kublik, S.</creator><creator>Bernau, C.</creator><creator>Loss, G. J.</creator><creator>Genuneit, J.</creator><creator>Horak, E.</creator><creator>Schloter, M.</creator><creator>Braun‐Fahrländer, C.</creator><creator>Danielewicz, H.</creator><creator>Heederik, D.</creator><creator>von Mutius, E.</creator><creator>Legatzki, A.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><orcidid>https://orcid.org/0000-0001-5764-1528</orcidid></search><sort><creationdate>201701</creationdate><title>Environmental and mucosal microbiota and their role in childhood asthma</title><author>Birzele, L. T. ; Depner, M. ; Ege, M. J. ; Engel, M. ; Kublik, S. ; Bernau, C. ; Loss, G. J. ; Genuneit, J. ; Horak, E. ; Schloter, M. ; Braun‐Fahrländer, C. ; Danielewicz, H. ; Heederik, D. ; von Mutius, E. ; Legatzki, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4212-9796bdda40f3c15dc8080cf223c66ad0668f1845c1a254dc351652b81a42c1af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Asthma</topic><topic>Asthma - epidemiology</topic><topic>Asthma - etiology</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Biodiversity</topic><topic>Child</topic><topic>Cross-Sectional Studies</topic><topic>Environmental Exposure - adverse effects</topic><topic>Environmental health</topic><topic>Environmental Microbiology</topic><topic>epidemiology</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Microbiology</topic><topic>microbiome</topic><topic>Microbiota</topic><topic>Mucous Membrane - microbiology</topic><topic>pediatrics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Birzele, L. T.</creatorcontrib><creatorcontrib>Depner, M.</creatorcontrib><creatorcontrib>Ege, M. J.</creatorcontrib><creatorcontrib>Engel, M.</creatorcontrib><creatorcontrib>Kublik, S.</creatorcontrib><creatorcontrib>Bernau, C.</creatorcontrib><creatorcontrib>Loss, G. J.</creatorcontrib><creatorcontrib>Genuneit, J.</creatorcontrib><creatorcontrib>Horak, E.</creatorcontrib><creatorcontrib>Schloter, M.</creatorcontrib><creatorcontrib>Braun‐Fahrländer, C.</creatorcontrib><creatorcontrib>Danielewicz, H.</creatorcontrib><creatorcontrib>Heederik, D.</creatorcontrib><creatorcontrib>von Mutius, E.</creatorcontrib><creatorcontrib>Legatzki, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Allergy (Copenhagen)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Birzele, L. T.</au><au>Depner, M.</au><au>Ege, M. J.</au><au>Engel, M.</au><au>Kublik, S.</au><au>Bernau, C.</au><au>Loss, G. J.</au><au>Genuneit, J.</au><au>Horak, E.</au><au>Schloter, M.</au><au>Braun‐Fahrländer, C.</au><au>Danielewicz, H.</au><au>Heederik, D.</au><au>von Mutius, E.</au><au>Legatzki, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Environmental and mucosal microbiota and their role in childhood asthma</atitle><jtitle>Allergy (Copenhagen)</jtitle><addtitle>Allergy</addtitle><date>2017-01</date><risdate>2017</risdate><volume>72</volume><issue>1</issue><spage>109</spage><epage>119</epage><pages>109-119</pages><issn>0105-4538</issn><eissn>1398-9995</eissn><abstract>Background
High microbial diversity in the environment has been associated with lower asthma risk, particularly in children exposed to farming. It remains unclear whether this effect operates through an altered microbiome of the mucosal surfaces of the airways.
Methods
DNA from mattress dust and nasal samples of 86 school age children was analyzed by 454 pyrosequencing of the 16S rRNA gene fragments. Based on operational taxonomic units (OTUs), bacterial diversity and composition were related to farm exposure and asthma status.
Results
Farm exposure was positively associated with bacterial diversity in mattress dust samples as determined by richness (P = 8.1 × 10−6) and Shannon index (P = 1.3 × 10−5). Despite considerable agreement of richness between mattress and nasal samples, the association of richness with farming in nasal samples was restricted to a high gradient of farm exposure, that is, exposure to cows and straw vs no exposure at all. In mattress dust, the genera Clostridium, Facklamia, an unclassified genus within the family of Ruminococcaceae, and six OTUs were positively associated with farming. Asthma was inversely associated with richness [aOR = 0.48 (0.22–1.02)] and Shannon index [aOR = 0.41 (0.21–0.83)] in mattress dust and to a lower extent in nasal samples [richness aOR 0.63 = (0.38–1.06), Shannon index aOR = 0.66 (0.39–1.12)].
Conclusion
The stronger inverse association of asthma with bacterial diversity in mattress dust as compared to nasal samples suggests microbial involvement beyond mere colonization of the upper airways. Whether inhalation of metabolites of environmental bacteria contributes to this phenomenon should be the focus of future research.</abstract><cop>Denmark</cop><pub>Blackwell Publishing Ltd</pub><pmid>27503830</pmid><doi>10.1111/all.13002</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-5764-1528</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Allergy (Copenhagen), 2017-01, Vol.72 (1), p.109-119 |
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| language | eng |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Asthma Asthma - epidemiology Asthma - etiology Bacteria - classification Bacteria - genetics Biodiversity Child Cross-Sectional Studies Environmental Exposure - adverse effects Environmental health Environmental Microbiology epidemiology Female Humans Male Microbiology microbiome Microbiota Mucous Membrane - microbiology pediatrics |
| title | Environmental and mucosal microbiota and their role in childhood asthma |
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