Lipid profiles of canine spermatozoa as revealed via matrix-assisted laser desorption/ionization mass spectrometry

Contents In this study, we investigated the ability of matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) to characterize the lipid contents of canine spermatozoa. For that, samples of pure semen were analysed. Indeed, quite comprehensive lipid coverage was observed, and the mo...

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Bibliographic Details
Published in:Reproduction in domestic animals 2016-12, Vol.51 (6), p.1055-1058
Main Authors: Braga Jr, LT, Bravo, NRS, Belaz, KRA, Zampieri, D, Eberlin, MN, Conforti, VA
Format: Article
Language:English
Subjects:
Animal reproduction
Animals
Desorption
Dogs
Dogs - physiology
Ions
Lasers
Lipid Metabolism
Lipids
Lipids - chemistry
Male
Mass spectrometry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - veterinary
Sperm
Spermatozoa - chemistry
Spermatozoa - physiology
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Summary:Contents In this study, we investigated the ability of matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) to characterize the lipid contents of canine spermatozoa. For that, samples of pure semen were analysed. Indeed, quite comprehensive lipid coverage was observed, and the most abundant phospholipid ions detected were from four phosphatidylcholines, that is those of m/z 760.6; 782.6; 808.6; and 830.6 and one of m/z 725.6 from a sphingomyelin. In conclusion, MALDI‐MS was found to offer an easy, fast, accurate, and sensitive analytical method for lipid profiling in canine spermatozoa and could be used as a tool to select sires by assessing the relationship between sperm lipid profiles and variables such as age and breeding history as well as to study the effects of cryopreservation on lipid contents.
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12792