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Comparison of catalytic activities of new La (III) and Ce (III) complexes with different diaza-crown ether ligands for DNA hydrolytic cleavage
A new diaza-crown ether with double hydroxypropyl branches (L = 1,1'-(1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diyl)dipropan-2-ol) and its two lanthanide complexes (LaL and CeL) were synthesised and characterised. Their nuclease activities on pUC19 DNA cleavage were investigated using...
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Published in: | Progress in reaction kinetics and mechanism 2016-01, Vol.41 (3), p.309-318 |
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creator | Li, Fang-zhen Xie, Jia-qing Cai, Shu-lan Feng, Fa-mei |
description | A new diaza-crown ether with double hydroxypropyl branches (L = 1,1'-(1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diyl)dipropan-2-ol) and its two lanthanide complexes (LaL and CeL) were synthesised and characterised. Their nuclease activities on pUC19 DNA cleavage were investigated using agarose gel electrophoresis, and pseudo-first-order rate constants kobs of 0.067 h–1 for LaL, 0.041 h–1 for CeL, 0.013 h–1 for LaL0, and 0.027 h–1 CeL0 were obtained (L0 = 1,4,10,13-tetraoxa-7,16-diazacyclooctadecane). The results indicated that the CeL complex was more efficient than the LaL complex for DNA cleavage at low concentrations, but the LaL complex was more efficient than the CeL complex at high concentrations. Moreover, kinetic studies demonstrated that introducing hydroxypropyl side arms in the ring of the diaza-crown ether can improve the catalytic ability of the complexes in DNA cleavage, which may be due to the hydroxypropyl side arms enhancing the bonding interaction between the complex and the DNA molecule by hydrogen bonding. A hydrolytic mechanism for DNA cleavage was demonstrated by applying several oxygen scavengers to the DNA catalytic cleavage. A proposed mechanism for the DNA cleavage shows that the hydroxypropyl side groups can recognise and bind the phosphodiester bond of DNA by hydrogen bonding and electrostatic interaction. |
doi_str_mv | 10.3184/146867816X14698928943442 |
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Their nuclease activities on pUC19 DNA cleavage were investigated using agarose gel electrophoresis, and pseudo-first-order rate constants kobs of 0.067 h–1 for LaL, 0.041 h–1 for CeL, 0.013 h–1 for LaL0, and 0.027 h–1 CeL0 were obtained (L0 = 1,4,10,13-tetraoxa-7,16-diazacyclooctadecane). The results indicated that the CeL complex was more efficient than the LaL complex for DNA cleavage at low concentrations, but the LaL complex was more efficient than the CeL complex at high concentrations. Moreover, kinetic studies demonstrated that introducing hydroxypropyl side arms in the ring of the diaza-crown ether can improve the catalytic ability of the complexes in DNA cleavage, which may be due to the hydroxypropyl side arms enhancing the bonding interaction between the complex and the DNA molecule by hydrogen bonding. A hydrolytic mechanism for DNA cleavage was demonstrated by applying several oxygen scavengers to the DNA catalytic cleavage. A proposed mechanism for the DNA cleavage shows that the hydroxypropyl side groups can recognise and bind the phosphodiester bond of DNA by hydrogen bonding and electrostatic interaction.</description><identifier>ISSN: 1468-6783</identifier><identifier>EISSN: 1471-406X</identifier><identifier>DOI: 10.3184/146867816X14698928943442</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Bonding ; Catalysis ; Catalysts ; Cleavage ; Deoxyribonucleic acid ; Ethers ; Hydrogen bonding ; Reaction kinetics</subject><ispartof>Progress in reaction kinetics and mechanism, 2016-01, Vol.41 (3), p.309-318</ispartof><rights>2016 SAGE Publications</rights><rights>Copyright Science Reviews 2000 Ltd 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-af97d5a5cacf67e72e6a9ba53d3cc7acebc1fd8c5bba342e9771d80185bd81263</citedby><cites>FETCH-LOGICAL-c399t-af97d5a5cacf67e72e6a9ba53d3cc7acebc1fd8c5bba342e9771d80185bd81263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.3184/146867816X14698928943442$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1817871924?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>315,786,790,21994,25783,27886,27957,27958,37047,37048,44625,44980,45368</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.3184/146867816X14698928943442?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc></links><search><creatorcontrib>Li, Fang-zhen</creatorcontrib><creatorcontrib>Xie, Jia-qing</creatorcontrib><creatorcontrib>Cai, Shu-lan</creatorcontrib><creatorcontrib>Feng, Fa-mei</creatorcontrib><title>Comparison of catalytic activities of new La (III) and Ce (III) complexes with different diaza-crown ether ligands for DNA hydrolytic cleavage</title><title>Progress in reaction kinetics and mechanism</title><description>A new diaza-crown ether with double hydroxypropyl branches (L = 1,1'-(1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diyl)dipropan-2-ol) and its two lanthanide complexes (LaL and CeL) were synthesised and characterised. Their nuclease activities on pUC19 DNA cleavage were investigated using agarose gel electrophoresis, and pseudo-first-order rate constants kobs of 0.067 h–1 for LaL, 0.041 h–1 for CeL, 0.013 h–1 for LaL0, and 0.027 h–1 CeL0 were obtained (L0 = 1,4,10,13-tetraoxa-7,16-diazacyclooctadecane). The results indicated that the CeL complex was more efficient than the LaL complex for DNA cleavage at low concentrations, but the LaL complex was more efficient than the CeL complex at high concentrations. Moreover, kinetic studies demonstrated that introducing hydroxypropyl side arms in the ring of the diaza-crown ether can improve the catalytic ability of the complexes in DNA cleavage, which may be due to the hydroxypropyl side arms enhancing the bonding interaction between the complex and the DNA molecule by hydrogen bonding. A hydrolytic mechanism for DNA cleavage was demonstrated by applying several oxygen scavengers to the DNA catalytic cleavage. A proposed mechanism for the DNA cleavage shows that the hydroxypropyl side groups can recognise and bind the phosphodiester bond of DNA by hydrogen bonding and electrostatic interaction.</description><subject>Bonding</subject><subject>Catalysis</subject><subject>Catalysts</subject><subject>Cleavage</subject><subject>Deoxyribonucleic acid</subject><subject>Ethers</subject><subject>Hydrogen bonding</subject><subject>Reaction kinetics</subject><issn>1468-6783</issn><issn>1471-406X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqNkU1rGzEQhpfSQlM3_0HQS3rYZvWx-jgGt0kMpr00kNsyqx3FCuuVK8lxnB_R3xwZ51ACpT3NO-J5Z9A7VUVo84VTLc6pkFoqTeVtUUYbpo3gQrA31QkVitaikbdvD1rqunD8ffUhpfumYZIpcVL9nof1BqJPYSLBEQsZxn32loDN_sFnj-nwPuGOLIGcLRaLzwSmgczxpbHFP-JjwXY-r8jgncOIUy4KnqC2MewmgnmFkYz-rlgTcSGSr98vyGo_xHDcZkeEB7jDj9U7B2PC05c6q24uv_2cX9fLH1eL-cWyttyYXIMzamihtWCdVKgYSjA9tHzg1iqw2FvqBm3bvgcuGBql6KAbqtt-0JRJPqvOjnM3MfzaYsrd2ieL4wgThm3qSrKtMtxI8x8oK2QjZVvQT6_Q-7CNU_lIoajSihomCqWPVMkmpYiu20S_hrjvaNMdbtr97abF2h6tqWT1x_B_-Z4BL9SkIA</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Li, Fang-zhen</creator><creator>Xie, Jia-qing</creator><creator>Cai, Shu-lan</creator><creator>Feng, Fa-mei</creator><general>SAGE Publications</general><general>Sage Publications Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>EHMNL</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>L7M</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7TM</scope></search><sort><creationdate>20160101</creationdate><title>Comparison of catalytic activities of new La (III) and Ce (III) complexes with different diaza-crown ether ligands for DNA hydrolytic cleavage</title><author>Li, Fang-zhen ; Xie, Jia-qing ; Cai, Shu-lan ; Feng, Fa-mei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-af97d5a5cacf67e72e6a9ba53d3cc7acebc1fd8c5bba342e9771d80185bd81263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Bonding</topic><topic>Catalysis</topic><topic>Catalysts</topic><topic>Cleavage</topic><topic>Deoxyribonucleic acid</topic><topic>Ethers</topic><topic>Hydrogen bonding</topic><topic>Reaction kinetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Fang-zhen</creatorcontrib><creatorcontrib>Xie, Jia-qing</creatorcontrib><creatorcontrib>Cai, Shu-lan</creatorcontrib><creatorcontrib>Feng, Fa-mei</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>UK & Ireland Database</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>Materials Research Database</collection><collection>ProQuest Materials Science Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Progress in reaction kinetics and mechanism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Li, Fang-zhen</au><au>Xie, Jia-qing</au><au>Cai, Shu-lan</au><au>Feng, Fa-mei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of catalytic activities of new La (III) and Ce (III) complexes with different diaza-crown ether ligands for DNA hydrolytic cleavage</atitle><jtitle>Progress in reaction kinetics and mechanism</jtitle><date>2016-01-01</date><risdate>2016</risdate><volume>41</volume><issue>3</issue><spage>309</spage><epage>318</epage><pages>309-318</pages><issn>1468-6783</issn><eissn>1471-406X</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>A new diaza-crown ether with double hydroxypropyl branches (L = 1,1'-(1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diyl)dipropan-2-ol) and its two lanthanide complexes (LaL and CeL) were synthesised and characterised. Their nuclease activities on pUC19 DNA cleavage were investigated using agarose gel electrophoresis, and pseudo-first-order rate constants kobs of 0.067 h–1 for LaL, 0.041 h–1 for CeL, 0.013 h–1 for LaL0, and 0.027 h–1 CeL0 were obtained (L0 = 1,4,10,13-tetraoxa-7,16-diazacyclooctadecane). The results indicated that the CeL complex was more efficient than the LaL complex for DNA cleavage at low concentrations, but the LaL complex was more efficient than the CeL complex at high concentrations. Moreover, kinetic studies demonstrated that introducing hydroxypropyl side arms in the ring of the diaza-crown ether can improve the catalytic ability of the complexes in DNA cleavage, which may be due to the hydroxypropyl side arms enhancing the bonding interaction between the complex and the DNA molecule by hydrogen bonding. A hydrolytic mechanism for DNA cleavage was demonstrated by applying several oxygen scavengers to the DNA catalytic cleavage. A proposed mechanism for the DNA cleavage shows that the hydroxypropyl side groups can recognise and bind the phosphodiester bond of DNA by hydrogen bonding and electrostatic interaction.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><doi>10.3184/146867816X14698928943442</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Bonding Catalysis Catalysts Cleavage Deoxyribonucleic acid Ethers Hydrogen bonding Reaction kinetics |
title | Comparison of catalytic activities of new La (III) and Ce (III) complexes with different diaza-crown ether ligands for DNA hydrolytic cleavage |
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