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Regulation of platelet lifespan in the presence and absence of thrombopoietin signaling
Essentials We examined platelet survival in models of absent or enhanced thrombopoietin (TPO) signaling. Platelet lifespan is normal in transgenic mice with chronically enhanced TPO signaling. Mpl deficiency does not negatively affect platelet lifespan in the absence of thrombocytopenia. We conclude...
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| Published in: | Journal of thrombosis and haemostasis 2016-09, Vol.14 (9), p.1882-1887 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c4587-c23625f859dd93be69df2f5b89ff8bb677701dfd96d1af16b334f5c1c17bc9683 |
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| cites | cdi_FETCH-LOGICAL-c4587-c23625f859dd93be69df2f5b89ff8bb677701dfd96d1af16b334f5c1c17bc9683 |
| container_end_page | 1887 |
| container_issue | 9 |
| container_start_page | 1882 |
| container_title | Journal of thrombosis and haemostasis |
| container_volume | 14 |
| creator | Lebois, M. Dowling, M. R. Gangatirkar, P. Hodgkin, P. D. Kile, B. T. Alexander, W. S. Josefsson, E. C. |
| description | Essentials
We examined platelet survival in models of absent or enhanced thrombopoietin (TPO) signaling.
Platelet lifespan is normal in transgenic mice with chronically enhanced TPO signaling.
Mpl deficiency does not negatively affect platelet lifespan in the absence of thrombocytopenia.
We conclude that TPO and its receptor Mpl are dispensable for platelet survival in adult mice.
Summary
Background
It is well established that thrombopoietin (TPO), acting via its receptor Mpl, is the major cytokine regulator of platelet biogenesis. The primary mechanism by which TPO signaling stimulates thrombopoiesis is via stimulation of Mpl‐expressing hematopoietic progenitors; Mpl on megakaryocytes and platelets acts to control the amount of TPO available. TPO could potentially reduce platelet and/or megakaryocyte apoptosis, and therefore increase the platelet count. However, the effect of TPO receptor signaling on platelet survival is unresolved.
Methods and results
Here, we investigated platelet survival in mouse models of absent or enhanced TPO signaling. In the absence of thrombocytopenia, Mpl deficiency did not negatively influence platelet lifespan, and nor was platelet survival affected in transgenic mice with chronically increased TPO signaling.
Conclusions
We conclude that TPO and its receptor Mpl are dispensable for platelet survival in adult mice. |
| doi_str_mv | 10.1111/jth.13397 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1823459059</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4192697231</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4587-c23625f859dd93be69df2f5b89ff8bb677701dfd96d1af16b334f5c1c17bc9683</originalsourceid><addsrcrecordid>eNp10EFLwzAcBfAgipvTg19ACl70sC1pmjY5ylCnDASZeCxJ-8-W0Ta1aZF9e7N18yCYS97hl0d4CF0TPCH-TDftekIoFckJGhJG-TjhND49ZkHpAF04t8GYCBbiczQIExpFmNAh-nyHVVfI1tgqsDqofYQC2qAwGlwtq8BUQbuGoG7AQZVBIKs8kKrP_kG7bmypbG0NtJ46s6pkYarVJTrTsnBwdbhH6OPpcTmbjxdvzy-zh8U4ixhPxllI45BpzkSeC6ogFrkONVNcaM2VipMkwSTXuYhzIjWJFaWRZhnJSKIyEXM6Qnd9b93Yrw5cm5bGZVAUsgLbuZTwkEZMYCY8vf1DN7Zr_Hf3Kox5iKOduu9V1ljnGtBp3ZhSNtuU4HS3durXTvdre3tzaOxUCfmvPM7rwbQH36aA7f9N6ety3lf-AAfdiUY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1822682049</pqid></control><display><type>article</type><title>Regulation of platelet lifespan in the presence and absence of thrombopoietin signaling</title><source>Wiley-Blackwell Journals</source><source>EZB Electronic Journals Library</source><creator>Lebois, M. ; Dowling, M. R. ; Gangatirkar, P. ; Hodgkin, P. D. ; Kile, B. T. ; Alexander, W. S. ; Josefsson, E. C.</creator><creatorcontrib>Lebois, M. ; Dowling, M. R. ; Gangatirkar, P. ; Hodgkin, P. D. ; Kile, B. T. ; Alexander, W. S. ; Josefsson, E. C.</creatorcontrib><description>Essentials
We examined platelet survival in models of absent or enhanced thrombopoietin (TPO) signaling.
Platelet lifespan is normal in transgenic mice with chronically enhanced TPO signaling.
Mpl deficiency does not negatively affect platelet lifespan in the absence of thrombocytopenia.
We conclude that TPO and its receptor Mpl are dispensable for platelet survival in adult mice.
Summary
Background
It is well established that thrombopoietin (TPO), acting via its receptor Mpl, is the major cytokine regulator of platelet biogenesis. The primary mechanism by which TPO signaling stimulates thrombopoiesis is via stimulation of Mpl‐expressing hematopoietic progenitors; Mpl on megakaryocytes and platelets acts to control the amount of TPO available. TPO could potentially reduce platelet and/or megakaryocyte apoptosis, and therefore increase the platelet count. However, the effect of TPO receptor signaling on platelet survival is unresolved.
Methods and results
Here, we investigated platelet survival in mouse models of absent or enhanced TPO signaling. In the absence of thrombocytopenia, Mpl deficiency did not negatively influence platelet lifespan, and nor was platelet survival affected in transgenic mice with chronically increased TPO signaling.
Conclusions
We conclude that TPO and its receptor Mpl are dispensable for platelet survival in adult mice.</description><identifier>ISSN: 1538-7933</identifier><identifier>ISSN: 1538-7836</identifier><identifier>EISSN: 1538-7836</identifier><identifier>DOI: 10.1111/jth.13397</identifier><identifier>PMID: 27344013</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>animal models ; Animals ; apoptosis ; Blood Platelets - cytology ; Blood Platelets - metabolism ; Cell Survival ; Female ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - metabolism ; Male ; Megakaryocytes - cytology ; Megakaryocytes - metabolism ; Mice ; Mice, Transgenic ; Mpl protein, mouse ; Platelet Count ; Platelet Transfusion ; platelets ; Ploidies ; Receptors, Thrombopoietin - metabolism ; Rodents ; Signal Transduction ; Thrombocytopenia ; Thrombopoiesis ; thrombopoietin ; Thrombopoietin - metabolism ; Transgenic animals</subject><ispartof>Journal of thrombosis and haemostasis, 2016-09, Vol.14 (9), p.1882-1887</ispartof><rights>2016 International Society on Thrombosis and Haemostasis</rights><rights>2016 International Society on Thrombosis and Haemostasis.</rights><rights>Copyright © 2016 International Society on Thrombosis and Haemostasis</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4587-c23625f859dd93be69df2f5b89ff8bb677701dfd96d1af16b334f5c1c17bc9683</citedby><cites>FETCH-LOGICAL-c4587-c23625f859dd93be69df2f5b89ff8bb677701dfd96d1af16b334f5c1c17bc9683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjth.13397$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjth.13397$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27344013$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lebois, M.</creatorcontrib><creatorcontrib>Dowling, M. R.</creatorcontrib><creatorcontrib>Gangatirkar, P.</creatorcontrib><creatorcontrib>Hodgkin, P. D.</creatorcontrib><creatorcontrib>Kile, B. T.</creatorcontrib><creatorcontrib>Alexander, W. S.</creatorcontrib><creatorcontrib>Josefsson, E. C.</creatorcontrib><title>Regulation of platelet lifespan in the presence and absence of thrombopoietin signaling</title><title>Journal of thrombosis and haemostasis</title><addtitle>J Thromb Haemost</addtitle><description>Essentials
We examined platelet survival in models of absent or enhanced thrombopoietin (TPO) signaling.
Platelet lifespan is normal in transgenic mice with chronically enhanced TPO signaling.
Mpl deficiency does not negatively affect platelet lifespan in the absence of thrombocytopenia.
We conclude that TPO and its receptor Mpl are dispensable for platelet survival in adult mice.
Summary
Background
It is well established that thrombopoietin (TPO), acting via its receptor Mpl, is the major cytokine regulator of platelet biogenesis. The primary mechanism by which TPO signaling stimulates thrombopoiesis is via stimulation of Mpl‐expressing hematopoietic progenitors; Mpl on megakaryocytes and platelets acts to control the amount of TPO available. TPO could potentially reduce platelet and/or megakaryocyte apoptosis, and therefore increase the platelet count. However, the effect of TPO receptor signaling on platelet survival is unresolved.
Methods and results
Here, we investigated platelet survival in mouse models of absent or enhanced TPO signaling. In the absence of thrombocytopenia, Mpl deficiency did not negatively influence platelet lifespan, and nor was platelet survival affected in transgenic mice with chronically increased TPO signaling.
Conclusions
We conclude that TPO and its receptor Mpl are dispensable for platelet survival in adult mice.</description><subject>animal models</subject><subject>Animals</subject><subject>apoptosis</subject><subject>Blood Platelets - cytology</subject><subject>Blood Platelets - metabolism</subject><subject>Cell Survival</subject><subject>Female</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - metabolism</subject><subject>Male</subject><subject>Megakaryocytes - cytology</subject><subject>Megakaryocytes - metabolism</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Mpl protein, mouse</subject><subject>Platelet Count</subject><subject>Platelet Transfusion</subject><subject>platelets</subject><subject>Ploidies</subject><subject>Receptors, Thrombopoietin - metabolism</subject><subject>Rodents</subject><subject>Signal Transduction</subject><subject>Thrombocytopenia</subject><subject>Thrombopoiesis</subject><subject>thrombopoietin</subject><subject>Thrombopoietin - metabolism</subject><subject>Transgenic animals</subject><issn>1538-7933</issn><issn>1538-7836</issn><issn>1538-7836</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp10EFLwzAcBfAgipvTg19ACl70sC1pmjY5ylCnDASZeCxJ-8-W0Ta1aZF9e7N18yCYS97hl0d4CF0TPCH-TDftekIoFckJGhJG-TjhND49ZkHpAF04t8GYCBbiczQIExpFmNAh-nyHVVfI1tgqsDqofYQC2qAwGlwtq8BUQbuGoG7AQZVBIKs8kKrP_kG7bmypbG0NtJ46s6pkYarVJTrTsnBwdbhH6OPpcTmbjxdvzy-zh8U4ixhPxllI45BpzkSeC6ogFrkONVNcaM2VipMkwSTXuYhzIjWJFaWRZhnJSKIyEXM6Qnd9b93Yrw5cm5bGZVAUsgLbuZTwkEZMYCY8vf1DN7Zr_Hf3Kox5iKOduu9V1ljnGtBp3ZhSNtuU4HS3durXTvdre3tzaOxUCfmvPM7rwbQH36aA7f9N6ety3lf-AAfdiUY</recordid><startdate>201609</startdate><enddate>201609</enddate><creator>Lebois, M.</creator><creator>Dowling, M. R.</creator><creator>Gangatirkar, P.</creator><creator>Hodgkin, P. D.</creator><creator>Kile, B. T.</creator><creator>Alexander, W. S.</creator><creator>Josefsson, E. C.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201609</creationdate><title>Regulation of platelet lifespan in the presence and absence of thrombopoietin signaling</title><author>Lebois, M. ; Dowling, M. R. ; Gangatirkar, P. ; Hodgkin, P. D. ; Kile, B. T. ; Alexander, W. S. ; Josefsson, E. C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4587-c23625f859dd93be69df2f5b89ff8bb677701dfd96d1af16b334f5c1c17bc9683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>animal models</topic><topic>Animals</topic><topic>apoptosis</topic><topic>Blood Platelets - cytology</topic><topic>Blood Platelets - metabolism</topic><topic>Cell Survival</topic><topic>Female</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Hematopoietic Stem Cells - metabolism</topic><topic>Male</topic><topic>Megakaryocytes - cytology</topic><topic>Megakaryocytes - metabolism</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Mpl protein, mouse</topic><topic>Platelet Count</topic><topic>Platelet Transfusion</topic><topic>platelets</topic><topic>Ploidies</topic><topic>Receptors, Thrombopoietin - metabolism</topic><topic>Rodents</topic><topic>Signal Transduction</topic><topic>Thrombocytopenia</topic><topic>Thrombopoiesis</topic><topic>thrombopoietin</topic><topic>Thrombopoietin - metabolism</topic><topic>Transgenic animals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lebois, M.</creatorcontrib><creatorcontrib>Dowling, M. R.</creatorcontrib><creatorcontrib>Gangatirkar, P.</creatorcontrib><creatorcontrib>Hodgkin, P. D.</creatorcontrib><creatorcontrib>Kile, B. T.</creatorcontrib><creatorcontrib>Alexander, W. S.</creatorcontrib><creatorcontrib>Josefsson, E. C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of thrombosis and haemostasis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lebois, M.</au><au>Dowling, M. R.</au><au>Gangatirkar, P.</au><au>Hodgkin, P. D.</au><au>Kile, B. T.</au><au>Alexander, W. S.</au><au>Josefsson, E. C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of platelet lifespan in the presence and absence of thrombopoietin signaling</atitle><jtitle>Journal of thrombosis and haemostasis</jtitle><addtitle>J Thromb Haemost</addtitle><date>2016-09</date><risdate>2016</risdate><volume>14</volume><issue>9</issue><spage>1882</spage><epage>1887</epage><pages>1882-1887</pages><issn>1538-7933</issn><issn>1538-7836</issn><eissn>1538-7836</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Essentials
We examined platelet survival in models of absent or enhanced thrombopoietin (TPO) signaling.
Platelet lifespan is normal in transgenic mice with chronically enhanced TPO signaling.
Mpl deficiency does not negatively affect platelet lifespan in the absence of thrombocytopenia.
We conclude that TPO and its receptor Mpl are dispensable for platelet survival in adult mice.
Summary
Background
It is well established that thrombopoietin (TPO), acting via its receptor Mpl, is the major cytokine regulator of platelet biogenesis. The primary mechanism by which TPO signaling stimulates thrombopoiesis is via stimulation of Mpl‐expressing hematopoietic progenitors; Mpl on megakaryocytes and platelets acts to control the amount of TPO available. TPO could potentially reduce platelet and/or megakaryocyte apoptosis, and therefore increase the platelet count. However, the effect of TPO receptor signaling on platelet survival is unresolved.
Methods and results
Here, we investigated platelet survival in mouse models of absent or enhanced TPO signaling. In the absence of thrombocytopenia, Mpl deficiency did not negatively influence platelet lifespan, and nor was platelet survival affected in transgenic mice with chronically increased TPO signaling.
Conclusions
We conclude that TPO and its receptor Mpl are dispensable for platelet survival in adult mice.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>27344013</pmid><doi>10.1111/jth.13397</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1538-7933 |
| ispartof | Journal of thrombosis and haemostasis, 2016-09, Vol.14 (9), p.1882-1887 |
| issn | 1538-7933 1538-7836 1538-7836 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1823459059 |
| source | Wiley-Blackwell Journals; EZB Electronic Journals Library |
| subjects | animal models Animals apoptosis Blood Platelets - cytology Blood Platelets - metabolism Cell Survival Female Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - metabolism Male Megakaryocytes - cytology Megakaryocytes - metabolism Mice Mice, Transgenic Mpl protein, mouse Platelet Count Platelet Transfusion platelets Ploidies Receptors, Thrombopoietin - metabolism Rodents Signal Transduction Thrombocytopenia Thrombopoiesis thrombopoietin Thrombopoietin - metabolism Transgenic animals |
| title | Regulation of platelet lifespan in the presence and absence of thrombopoietin signaling |
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