Immobilization of invertase on chitosan coated γ-Fe2O3 magnetic nanoparticles to facilitate magnetic separation

[Display omitted] Industrially important invertase enzyme was immobilized on chitosan coated sol gel derived γ-Fe2O3 magnetic nanoparticles (MNPs) to enable it for repetitive use by magnetic separation. MNPs were characterized by X-ray diffraction (XRD), dynamic light scattering (DLS), field emissio...

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Bibliographic Details
Published in:Journal of colloid and interface science 2016-11, Vol.482, p.159-164
Main Authors: Waifalkar, P.P., Parit, S.B., Chougale, A.D., Sahoo, Subasa C., Patil, P.S., Patil, P.B.
Format: Article
Language:English
Subjects:
beta-Fructofuranosidase - chemistry
Chemical Fractionation - methods
Chitosan - chemistry
Enzymes, Immobilized - chemistry
Equipment Reuse
Ferric Compounds - chemistry
Hydrogen-Ion Concentration
Invertase
Magnetic properties
Magnetic separation
Magnetite Nanoparticles - chemistry
Magnetite Nanoparticles - ultrastructure
Magnets
Phase Transition
Sol–gel processes
Sucrose - chemistry
Temperature
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Summary:[Display omitted] Industrially important invertase enzyme was immobilized on chitosan coated sol gel derived γ-Fe2O3 magnetic nanoparticles (MNPs) to enable it for repetitive use by magnetic separation. MNPs were characterized by X-ray diffraction (XRD), dynamic light scattering (DLS), field emission scanning electron microscope (FE-SEM), Fourier transform infrared (FTIR) spectrometer and magnetic measurements. FTIR studies confirmed successful immobilization of invertase on MNPs. The ability to convert sucrose into invert syrup was enhanced in immobilized invertase compared to that of free enzyme. Further it was found that invertase immobilized on MNPs (IIMNPs) were more stable at varying pH and temperature conditions. Magnetic separation technique was successfully employed for reuse of the IIMNPs for 20 times without significant loss of activity.
ISSN:0021-9797
1095-7103
DOI:10.1016/j.jcis.2016.07.082