Ablative fractional laser enhances MAL-induced PpIX accumulation: Impact of laser channel density, incubation time and drug concentration

Pretreatment of skin with ablative fractional laser enhances accumulation of topical provided photosensitizer, but essential information is lacking on the interaction between laser channel densities and pharmacokinetics. Hence our objectives were to investigate how protoporphyrin accumulation was af...

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Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2016-06, Vol.159, p.42-48
Main Authors: Haak, C.S., Christiansen, K., Erlendsson, A.M., Taudorf, E.H., Thaysen-Petersen, D., Wulf, H.C., Haedersdal, M.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aminolevulinic Acid - administration & dosage
Aminolevulinic Acid - analogs & derivatives
Aminolevulinic Acid - metabolism
Drug delivery
Fractional Er:YAG laser
Humans
Lasers
Male
Methyl aminolevulinate
Photosensitizer
Photosensitizing Agents - administration & dosage
Photosensitizing Agents - metabolism
Protoporphyrins - metabolism
Spectrometry, Fluorescence
Young Adult
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Summary:Pretreatment of skin with ablative fractional laser enhances accumulation of topical provided photosensitizer, but essential information is lacking on the interaction between laser channel densities and pharmacokinetics. Hence our objectives were to investigate how protoporphyrin accumulation was affected by laser densities, incubation time and drug concentration. We conducted the study on the back of healthy male volunteers (n=11). Test areas were pretreated with 2940nm ablative fractional Er:YAG laser, 11.2mJ per laser channel using densities of 1, 2, 5, 10 and 15% (AFL 1–15%). Control areas received pretreatment with curettage or no pretreatment. Methyl aminolevulinate (MAL) was applied under occlusion in concentrations of 0, 80 and 160mg/g. MAL-induced protoporphyrin fluorescence was quantified with a handheld photometer after 0, 30, 60, 120 and 180min incubation. The individual fluorescence intensity reached from the highest density (15%) and longest MAL 160mg/g incubation time (180min) was selected as reference (100%) for other interventional measurements. A low laser density of 1% markedly enhanced fluorescence intensities from 34% to 75% (no pretreatment vs. AFL 1%, MAL 160mg/g, 180min; p0.0537, 30–180min). MAL-induced protoporphyrin accumulation is augmented by enhancing AFL densities up to 5%. Further, this model indicates that incubation time as well as drug concentration of MAL may be reduced with laser pretreatment. •Ablative fractional laser (AFL) enhances accumulation of topical provided photosensitizer.•PpIX formation is accelerated by AFL-exposure in healthy human skin.•Protoporfyrin IX (PpIX) levels increase with AFL densities up to 5% coverage.•Laser densities above 5% exert a minor additional effect on PpIX accumulation.•Methyl aminolevulinate 80 and 160mg/g induce similar PpIX levels after AFL 1–5%.
ISSN:1011-1344
1873-2682
DOI:10.1016/j.jphotobiol.2016.03.021