MicroRNA-409-3p inhibits osteosarcoma cell migration and invasion by targeting catenin-δ1

Osteosarcoma is the most common primary bone cancer which is associated with early metastatic potential and poor prognosis. However, the molecular mechanisms underlying osteosarcoma progression are not well characterized. Here, we investigated the role of miR-409-3p in osteosarcoma metastasis. Osteo...

Full description

Saved in:
Bibliographic Details
Published in:Gene 2016-06, Vol.584 (1), p.83-89
Main Authors: Wu, Shifeng, Du, Xinjie, Wu, Manwu, Du, Hechun, Shi, Xiaoliang, Zhang, Tao
Format: Article
Language:English
Subjects:
Catenin-δ1
Catenins - genetics
Cell invasion
Cell Line, Tumor
Cell migration
Down-Regulation
Humans
Metastasis
MicroRNAs - physiology
MiR-409-3p
Neoplasm Invasiveness - genetics
Neoplasm Metastasis - genetics
Osteosarcoma
Osteosarcoma - pathology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c271t-a4fbf73d235a43d15a775b52b351f960c304e571fce81e4599d8a190cc3966d83
cites cdi_FETCH-LOGICAL-c271t-a4fbf73d235a43d15a775b52b351f960c304e571fce81e4599d8a190cc3966d83
container_end_page 89
container_issue 1
container_start_page 83
container_title Gene
container_volume 584
creator Wu, Shifeng
Du, Xinjie
Wu, Manwu
Du, Hechun
Shi, Xiaoliang
Zhang, Tao
description Osteosarcoma is the most common primary bone cancer which is associated with early metastatic potential and poor prognosis. However, the molecular mechanisms underlying osteosarcoma progression are not well characterized. Here, we investigated the role of miR-409-3p in osteosarcoma metastasis. Osteosarcoma tissue showed decreased expression of miR-409-3p compared to adjacent non-tumorous tissue. The expression level of miR-409-3p was negatively correlated with osteosarcoma metastasis. Overexpression of miR-409-3p in osteosarcoma cells (U2OS) inhibited cell migration and invasion. Bioinformatics analysis showed that catenin-δ1 (CTNND1, p120-catenin) is a direct target of miR-409-3p. Overexpression of miR-409-3p repressed the expression of catenin-δ1 in U2OS cells at both mRNA and protein levels. Meanwhile, miR-409-3p repressed the activity of luciferase reporter containing the 3′-untranslated region (3′UTR) of CTNND1 gene. Furthermore, expression of catenin-δ1 rescued the inhibitory effect of miR-409-3p on cell migration and invasion. Altogether, these results indicated that miR-409-3p targets catenin-δ1 to repress osteosarcoma metastasis. •Downregulation of miR-409-3p expression is associated with osteosarcoma metastasis.•MiR-409-3p suppresses osteosarcoma cell migration and invasion.•Catenin-δ1 is a direct target of miR-409-3p in osteosarcoma cells.•Catenin-δ1 rescues the inhibitory effects of miR-409-3p on cell migration and invasion.
doi_str_mv 10.1016/j.gene.2016.03.021
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1779414269</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378111916301664</els_id><sourcerecordid>1779414269</sourcerecordid><originalsourceid>FETCH-LOGICAL-c271t-a4fbf73d235a43d15a775b52b351f960c304e571fce81e4599d8a190cc3966d83</originalsourceid><addsrcrecordid>eNp9kMFO3DAURa0K1Blof6CLKks2CX52HMdSNwhBQRqohOimG8txXqYeTZyp7UHiv_iOflMdDXSJN7al867ePYR8AVoBheZ8U63RY8Xyu6K8ogw-kCW0UpWU8vaILCmXbQkAakFOYtzQfIRgH8mCNUqxhssl-XXnbJge7i_KmqqS7wrnf7vOpVhMMeEUTbDTaAqL220xunUwyU2-ML7P4JOJ86d7LpIJa0zOrwtrEnrny78v8IkcD2Yb8fPrfUp-Xl89Xt6Uqx_fby8vVqVlElJp6qEbJO8ZF6bmPQgjpegE67iAQTXUclqjkDBYbAFroVTfGlDUWq6apm_5KTk75O7C9GePMenRxXlh43HaRw1Sqhrq3Dmj7IDmzjEGHPQuuNGEZw1Uz071Rs9O9exUU66z0zz09TV_343Y_x95k5iBbwcAc8snh0FH69Bb7F1Am3Q_uffy_wG49Yez</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1779414269</pqid></control><display><type>article</type><title>MicroRNA-409-3p inhibits osteosarcoma cell migration and invasion by targeting catenin-δ1</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Wu, Shifeng ; Du, Xinjie ; Wu, Manwu ; Du, Hechun ; Shi, Xiaoliang ; Zhang, Tao</creator><creatorcontrib>Wu, Shifeng ; Du, Xinjie ; Wu, Manwu ; Du, Hechun ; Shi, Xiaoliang ; Zhang, Tao</creatorcontrib><description>Osteosarcoma is the most common primary bone cancer which is associated with early metastatic potential and poor prognosis. However, the molecular mechanisms underlying osteosarcoma progression are not well characterized. Here, we investigated the role of miR-409-3p in osteosarcoma metastasis. Osteosarcoma tissue showed decreased expression of miR-409-3p compared to adjacent non-tumorous tissue. The expression level of miR-409-3p was negatively correlated with osteosarcoma metastasis. Overexpression of miR-409-3p in osteosarcoma cells (U2OS) inhibited cell migration and invasion. Bioinformatics analysis showed that catenin-δ1 (CTNND1, p120-catenin) is a direct target of miR-409-3p. Overexpression of miR-409-3p repressed the expression of catenin-δ1 in U2OS cells at both mRNA and protein levels. Meanwhile, miR-409-3p repressed the activity of luciferase reporter containing the 3′-untranslated region (3′UTR) of CTNND1 gene. Furthermore, expression of catenin-δ1 rescued the inhibitory effect of miR-409-3p on cell migration and invasion. Altogether, these results indicated that miR-409-3p targets catenin-δ1 to repress osteosarcoma metastasis. •Downregulation of miR-409-3p expression is associated with osteosarcoma metastasis.•MiR-409-3p suppresses osteosarcoma cell migration and invasion.•Catenin-δ1 is a direct target of miR-409-3p in osteosarcoma cells.•Catenin-δ1 rescues the inhibitory effects of miR-409-3p on cell migration and invasion.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/j.gene.2016.03.021</identifier><identifier>PMID: 26992637</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Catenin-δ1 ; Catenins - genetics ; Cell invasion ; Cell Line, Tumor ; Cell migration ; Down-Regulation ; Humans ; Metastasis ; MicroRNAs - physiology ; MiR-409-3p ; Neoplasm Invasiveness - genetics ; Neoplasm Metastasis - genetics ; Osteosarcoma ; Osteosarcoma - pathology</subject><ispartof>Gene, 2016-06, Vol.584 (1), p.83-89</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c271t-a4fbf73d235a43d15a775b52b351f960c304e571fce81e4599d8a190cc3966d83</citedby><cites>FETCH-LOGICAL-c271t-a4fbf73d235a43d15a775b52b351f960c304e571fce81e4599d8a190cc3966d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26992637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Shifeng</creatorcontrib><creatorcontrib>Du, Xinjie</creatorcontrib><creatorcontrib>Wu, Manwu</creatorcontrib><creatorcontrib>Du, Hechun</creatorcontrib><creatorcontrib>Shi, Xiaoliang</creatorcontrib><creatorcontrib>Zhang, Tao</creatorcontrib><title>MicroRNA-409-3p inhibits osteosarcoma cell migration and invasion by targeting catenin-δ1</title><title>Gene</title><addtitle>Gene</addtitle><description>Osteosarcoma is the most common primary bone cancer which is associated with early metastatic potential and poor prognosis. However, the molecular mechanisms underlying osteosarcoma progression are not well characterized. Here, we investigated the role of miR-409-3p in osteosarcoma metastasis. Osteosarcoma tissue showed decreased expression of miR-409-3p compared to adjacent non-tumorous tissue. The expression level of miR-409-3p was negatively correlated with osteosarcoma metastasis. Overexpression of miR-409-3p in osteosarcoma cells (U2OS) inhibited cell migration and invasion. Bioinformatics analysis showed that catenin-δ1 (CTNND1, p120-catenin) is a direct target of miR-409-3p. Overexpression of miR-409-3p repressed the expression of catenin-δ1 in U2OS cells at both mRNA and protein levels. Meanwhile, miR-409-3p repressed the activity of luciferase reporter containing the 3′-untranslated region (3′UTR) of CTNND1 gene. Furthermore, expression of catenin-δ1 rescued the inhibitory effect of miR-409-3p on cell migration and invasion. Altogether, these results indicated that miR-409-3p targets catenin-δ1 to repress osteosarcoma metastasis. •Downregulation of miR-409-3p expression is associated with osteosarcoma metastasis.•MiR-409-3p suppresses osteosarcoma cell migration and invasion.•Catenin-δ1 is a direct target of miR-409-3p in osteosarcoma cells.•Catenin-δ1 rescues the inhibitory effects of miR-409-3p on cell migration and invasion.</description><subject>Catenin-δ1</subject><subject>Catenins - genetics</subject><subject>Cell invasion</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Down-Regulation</subject><subject>Humans</subject><subject>Metastasis</subject><subject>MicroRNAs - physiology</subject><subject>MiR-409-3p</subject><subject>Neoplasm Invasiveness - genetics</subject><subject>Neoplasm Metastasis - genetics</subject><subject>Osteosarcoma</subject><subject>Osteosarcoma - pathology</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp9kMFO3DAURa0K1Blof6CLKks2CX52HMdSNwhBQRqohOimG8txXqYeTZyp7UHiv_iOflMdDXSJN7al867ePYR8AVoBheZ8U63RY8Xyu6K8ogw-kCW0UpWU8vaILCmXbQkAakFOYtzQfIRgH8mCNUqxhssl-XXnbJge7i_KmqqS7wrnf7vOpVhMMeEUTbDTaAqL220xunUwyU2-ML7P4JOJ86d7LpIJa0zOrwtrEnrny78v8IkcD2Yb8fPrfUp-Xl89Xt6Uqx_fby8vVqVlElJp6qEbJO8ZF6bmPQgjpegE67iAQTXUclqjkDBYbAFroVTfGlDUWq6apm_5KTk75O7C9GePMenRxXlh43HaRw1Sqhrq3Dmj7IDmzjEGHPQuuNGEZw1Uz071Rs9O9exUU66z0zz09TV_343Y_x95k5iBbwcAc8snh0FH69Bb7F1Am3Q_uffy_wG49Yez</recordid><startdate>20160610</startdate><enddate>20160610</enddate><creator>Wu, Shifeng</creator><creator>Du, Xinjie</creator><creator>Wu, Manwu</creator><creator>Du, Hechun</creator><creator>Shi, Xiaoliang</creator><creator>Zhang, Tao</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160610</creationdate><title>MicroRNA-409-3p inhibits osteosarcoma cell migration and invasion by targeting catenin-δ1</title><author>Wu, Shifeng ; Du, Xinjie ; Wu, Manwu ; Du, Hechun ; Shi, Xiaoliang ; Zhang, Tao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c271t-a4fbf73d235a43d15a775b52b351f960c304e571fce81e4599d8a190cc3966d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Catenin-δ1</topic><topic>Catenins - genetics</topic><topic>Cell invasion</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Down-Regulation</topic><topic>Humans</topic><topic>Metastasis</topic><topic>MicroRNAs - physiology</topic><topic>MiR-409-3p</topic><topic>Neoplasm Invasiveness - genetics</topic><topic>Neoplasm Metastasis - genetics</topic><topic>Osteosarcoma</topic><topic>Osteosarcoma - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Shifeng</creatorcontrib><creatorcontrib>Du, Xinjie</creatorcontrib><creatorcontrib>Wu, Manwu</creatorcontrib><creatorcontrib>Du, Hechun</creatorcontrib><creatorcontrib>Shi, Xiaoliang</creatorcontrib><creatorcontrib>Zhang, Tao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Shifeng</au><au>Du, Xinjie</au><au>Wu, Manwu</au><au>Du, Hechun</au><au>Shi, Xiaoliang</au><au>Zhang, Tao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA-409-3p inhibits osteosarcoma cell migration and invasion by targeting catenin-δ1</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2016-06-10</date><risdate>2016</risdate><volume>584</volume><issue>1</issue><spage>83</spage><epage>89</epage><pages>83-89</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Osteosarcoma is the most common primary bone cancer which is associated with early metastatic potential and poor prognosis. However, the molecular mechanisms underlying osteosarcoma progression are not well characterized. Here, we investigated the role of miR-409-3p in osteosarcoma metastasis. Osteosarcoma tissue showed decreased expression of miR-409-3p compared to adjacent non-tumorous tissue. The expression level of miR-409-3p was negatively correlated with osteosarcoma metastasis. Overexpression of miR-409-3p in osteosarcoma cells (U2OS) inhibited cell migration and invasion. Bioinformatics analysis showed that catenin-δ1 (CTNND1, p120-catenin) is a direct target of miR-409-3p. Overexpression of miR-409-3p repressed the expression of catenin-δ1 in U2OS cells at both mRNA and protein levels. Meanwhile, miR-409-3p repressed the activity of luciferase reporter containing the 3′-untranslated region (3′UTR) of CTNND1 gene. Furthermore, expression of catenin-δ1 rescued the inhibitory effect of miR-409-3p on cell migration and invasion. Altogether, these results indicated that miR-409-3p targets catenin-δ1 to repress osteosarcoma metastasis. •Downregulation of miR-409-3p expression is associated with osteosarcoma metastasis.•MiR-409-3p suppresses osteosarcoma cell migration and invasion.•Catenin-δ1 is a direct target of miR-409-3p in osteosarcoma cells.•Catenin-δ1 rescues the inhibitory effects of miR-409-3p on cell migration and invasion.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26992637</pmid><doi>10.1016/j.gene.2016.03.021</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0378-1119
ispartof Gene, 2016-06, Vol.584 (1), p.83-89
issn 0378-1119
1879-0038
language eng
recordid cdi_proquest_miscellaneous_1779414269
source ScienceDirect Freedom Collection 2022-2024
subjects Catenin-δ1
Catenins - genetics
Cell invasion
Cell Line, Tumor
Cell migration
Down-Regulation
Humans
Metastasis
MicroRNAs - physiology
MiR-409-3p
Neoplasm Invasiveness - genetics
Neoplasm Metastasis - genetics
Osteosarcoma
Osteosarcoma - pathology
title MicroRNA-409-3p inhibits osteosarcoma cell migration and invasion by targeting catenin-δ1
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-23T04%3A20%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=MicroRNA-409-3p%20inhibits%20osteosarcoma%20cell%20migration%20and%20invasion%20by%20targeting%20catenin-%CE%B41&rft.jtitle=Gene&rft.au=Wu,%20Shifeng&rft.date=2016-06-10&rft.volume=584&rft.issue=1&rft.spage=83&rft.epage=89&rft.pages=83-89&rft.issn=0378-1119&rft.eissn=1879-0038&rft_id=info:doi/10.1016/j.gene.2016.03.021&rft_dat=%3Cproquest_cross%3E1779414269%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c271t-a4fbf73d235a43d15a775b52b351f960c304e571fce81e4599d8a190cc3966d83%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1779414269&rft_id=info:pmid/26992637&rfr_iscdi=true