The relative antimicrobial effect of blue 405 nm LED and blue 405 nm laser on methicillin-resistant Staphylococcus aureus in vitro

It has long been argued that light from a laser diode is superior to light from a light-emitting diode (LED) in terms of its effect on biological tissues. In order to shed light on this ongoing debate, we compared the antimicrobial effect of light emitted from a 405-nm LED with that of a 405-nm lase...

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Bibliographic Details
Published in:Lasers in medical science 2015-12, Vol.30 (9), p.2265-2271
Main Authors: Masson-Meyers, Daniela Santos, Bumah, Violet Vakunseh, Biener, Gabriel, Raicu, Valerica, Enwemeka, Chukuka Samuel
Format: Article
Language:English
Subjects:
Antiinfectives and antibacterials
Antimicrobial agents
Bacteria
Bacterial Load
Dentistry
Fluence
Irradiation
Lasers
Light (visible radiation)
Light emitting diodes
Light therapy
Medicine
Medicine & Public Health
Methicillin-Resistant Staphylococcus aureus - physiology
Methicillin-Resistant Staphylococcus aureus - radiation effects
MRSA
Optical Devices
Optics
Original Article
Photonics
Quantum Optics
Semiconductors
Staphylococcus aureus
Staphylococcus infections
Time Factors
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Summary:It has long been argued that light from a laser diode is superior to light from a light-emitting diode (LED) in terms of its effect on biological tissues. In order to shed light on this ongoing debate, we compared the antimicrobial effect of light emitted from a 405-nm LED with that of a 405-nm laser on methicillin-resistant Staphylococcus aureus (MRSA) at comparable fluences. We cultured 5 × 10 6  CFU/ml MRSA on tryptic soy agar and then irradiated culture plates once, twice, or thrice with either LED or laser light using 40, 54, 81, or 121 J/cm 2 fluence at 15-, 30-, or 240-min time interval between irradiation. Cultures were incubated immediately after irradiation at 37 °C for 24 h before imaging and counting remnant bacterial colonies. Regardless of the device used, LED or laser, irradiation at each fluence resulted in statistically significant bacterial growth suppression compared to non-irradiated controls ( p  
ISSN:0268-8921
1435-604X
DOI:10.1007/s10103-015-1799-1