Ultrafast laser microfabrication of a trapping device for colorectal cancer cells

[Display omitted] •Polycarbonate is fabricated using CNC engraving machine to make microfluidic channel.•We prepared gelatin-coated PET membrane.•We make cancer cell capture device with polycarbonate plate and PET membrane.•PET membrane is attached on the polycarbonate plate and negative pressure is...

Full description

Saved in:
Bibliographic Details
Published in:Microelectronic engineering 2015-06, Vol.140, p.1-5
Main Authors: Kim, Kwang-Ryul, Kim, Hyun-Jin, Choi, Hyun-Il, Shin, Keong-Sub, Cho, Sung-Hak, Choi, Byoung-Deog
Format: Article
Language:English
Subjects:
Cancer
Cell trapping device
Channels
Colorectal cancer cells
Devices
Lasers
Membranes
Microfluidics
Microholes
Polyethylene terephthalates
Ultrafast laser
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] •Polycarbonate is fabricated using CNC engraving machine to make microfluidic channel.•We prepared gelatin-coated PET membrane.•We make cancer cell capture device with polycarbonate plate and PET membrane.•PET membrane is attached on the polycarbonate plate and negative pressure is applied on microfluidic channel.•We successfully developed the new singular cell capture device for cancer cell manipulation. Although celltrapping devices have been microfabricated and widely used for manipulation of bio cells, devices that trap multiple cells simultaneously are difficult to design because fabrication is complicated and time consuming. We designed and manufactured a microfluidic device using a polycarbonate plate with a flatness less than 200μm and a gelatin-coated polyethylene terephthalate (PET) membrane. The device was used to capture colorectal cancer cells from one of the most common types of human malignant tumors. Microfluidic channels for the device were micromachined in minutes using a Computerized Numerically Controlled (CNC) engraving machine. We microfabricated multiple microholes on the PET membrane, which had a thickness of 13μm, using an ultrafast, 1025nm diode-pumped solid state femtosecond laser. The 100 microholes were drilled by spirally moving spot size of 4μm laser beam. It is very important to obtain smooth and clean surface to avoid cell damages when they are trapped on the device. The relationship between the diameter changes of the microholes and variations in laser output power as well as laser fluence were investigated through parametric analysis. The average diameter of the holes increased exponentially with laser power. The gelatin-coated PET membrane was attached to the polycarbonate device and a syringe with a tube controlled negative pressure inside the channels of the cell-trapping device. Maintaining negative pressure inside the channels under the microholes on the PET membrane, colorectal cancer cells were dropped using the cell dropping pipette and successfully captured for manipulation under same environmental condition.
ISSN:0167-9317
1873-5568
DOI:10.1016/j.mee.2015.04.118