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E2 potentializes benzo(a)pyrene-induced hepatic cytochrome P450 enzyme activities in Nile tilapia at high concentrations
In the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17β-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia (Oreochromis niloticus) were exposed f...
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| Published in: | Environmental science and pollution research international 2015-11, Vol.22 (22), p.17367-17374 |
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| creator | Rodrigues, Aline Cristina Ferreira de Oliveira Moneró, Tatiana Frighetto, Rosa Toyoko Shiraishi de Almeida, Eduardo Alves |
| description | In the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17β-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia (Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L⁻¹) and E2 (5 μg L⁻¹). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolated BaP treatment increased GST activity after 10 days. However, this response was not observed in the mixture treatment, suggesting that E2 suppressed the GST induction modulated by BaP. The results put together indicated that E2 altered the biotransformation pathway regarding enzymes activated by BaP in Nile tilapia. |
| doi_str_mv | 10.1007/s11356-014-3670-5 |
| format | article |
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In this study, Nile tilapia (Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L⁻¹) and E2 (5 μg L⁻¹). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolated BaP treatment increased GST activity after 10 days. However, this response was not observed in the mixture treatment, suggesting that E2 suppressed the GST induction modulated by BaP. The results put together indicated that E2 altered the biotransformation pathway regarding enzymes activated by BaP in Nile tilapia.</description><identifier>ISSN: 0944-1344</identifier><identifier>EISSN: 1614-7499</identifier><identifier>DOI: 10.1007/s11356-014-3670-5</identifier><identifier>PMID: 25280508</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>17β-Estradiol ; Animals ; Aquatic ecosystems ; Aquatic environment ; Aquatic Pollution ; Atmospheric Protection/Air Quality Control/Air Pollution ; Benzo(a)pyrene ; Benzo(a)pyrene - toxicity ; Biomarkers ; Biomarkers - metabolism ; Biotransformation ; Biotransformation - drug effects ; Chemicals ; Cichlids - metabolism ; Combined treatment ; Cytochrome ; cytochrome P-450 ; Cytochrome P450 ; Dose-Response Relationship, Drug ; Drug Synergism ; Earth and Environmental Science ; Ecotoxicology ; Environment ; Environmental Chemistry ; Environmental Health ; Enzymatic activity ; enzyme activity ; Enzymes ; estradiol ; Estradiol - pharmacology ; Estrogens ; fish ; Genes ; Glutathione transferase ; Liver - drug effects ; Liver - enzymology ; Liver - metabolism ; Metabolism ; Metabolites ; Molecular and Cellular Effects of Contamination in Aquatic Ecosystems ; Oreochromis niloticus ; Pesticides ; polycyclic aromatic hydrocarbons ; Pyrene ; Synergism ; Tilapia ; Time Factors ; Waste Water Technology ; Water Management ; Water Pollutants, Chemical - toxicity ; Water Pollution Control</subject><ispartof>Environmental science and pollution research international, 2015-11, Vol.22 (22), p.17367-17374</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><rights>Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-200d137624a1dee8be30056e0376735af97cb7591e14d9d9dfbb8f9c53c7ca353</citedby><cites>FETCH-LOGICAL-c536t-200d137624a1dee8be30056e0376735af97cb7591e14d9d9dfbb8f9c53c7ca353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1788599661/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1788599661?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>315,786,790,11715,27957,27958,36095,36096,44398,75252</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25280508$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodrigues, Aline Cristina Ferreira</creatorcontrib><creatorcontrib>de Oliveira Moneró, Tatiana</creatorcontrib><creatorcontrib>Frighetto, Rosa Toyoko Shiraishi</creatorcontrib><creatorcontrib>de Almeida, Eduardo Alves</creatorcontrib><title>E2 potentializes benzo(a)pyrene-induced hepatic cytochrome P450 enzyme activities in Nile tilapia at high concentrations</title><title>Environmental science and pollution research international</title><addtitle>Environ Sci Pollut Res</addtitle><addtitle>Environ Sci Pollut Res Int</addtitle><description>In the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17β-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia (Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L⁻¹) and E2 (5 μg L⁻¹). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolated BaP treatment increased GST activity after 10 days. However, this response was not observed in the mixture treatment, suggesting that E2 suppressed the GST induction modulated by BaP. The results put together indicated that E2 altered the biotransformation pathway regarding enzymes activated by BaP in Nile tilapia.</description><subject>17β-Estradiol</subject><subject>Animals</subject><subject>Aquatic ecosystems</subject><subject>Aquatic environment</subject><subject>Aquatic Pollution</subject><subject>Atmospheric Protection/Air Quality Control/Air Pollution</subject><subject>Benzo(a)pyrene</subject><subject>Benzo(a)pyrene - toxicity</subject><subject>Biomarkers</subject><subject>Biomarkers - metabolism</subject><subject>Biotransformation</subject><subject>Biotransformation - drug effects</subject><subject>Chemicals</subject><subject>Cichlids - metabolism</subject><subject>Combined treatment</subject><subject>Cytochrome</subject><subject>cytochrome P-450</subject><subject>Cytochrome P450</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Synergism</subject><subject>Earth and Environmental Science</subject><subject>Ecotoxicology</subject><subject>Environment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Enzymatic activity</subject><subject>enzyme activity</subject><subject>Enzymes</subject><subject>estradiol</subject><subject>Estradiol - pharmacology</subject><subject>Estrogens</subject><subject>fish</subject><subject>Genes</subject><subject>Glutathione transferase</subject><subject>Liver - drug effects</subject><subject>Liver - enzymology</subject><subject>Liver - metabolism</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Molecular and Cellular Effects of Contamination in Aquatic Ecosystems</subject><subject>Oreochromis niloticus</subject><subject>Pesticides</subject><subject>polycyclic aromatic hydrocarbons</subject><subject>Pyrene</subject><subject>Synergism</subject><subject>Tilapia</subject><subject>Time Factors</subject><subject>Waste Water Technology</subject><subject>Water Management</subject><subject>Water Pollutants, Chemical - toxicity</subject><subject>Water Pollution Control</subject><issn>0944-1344</issn><issn>1614-7499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>M0C</sourceid><recordid>eNp9kUFv1DAQhSMEotvCD-AClriUQ2AmtuPkWFUtIFWABD1bjjPZdZW1g50gtr8er1IQ4oB8GNvzvTcjvaJ4gfAWAdS7hMhlXQKKktcKSvmo2GCdX0q07eNiA60QJXIhTorTlO4AKmgr9bQ4qWTVgIRmU_y8qtgUZvKzM6O7p8Q68vfh3LyZDpE8lc73i6We7Wgys7PMHuZgdzHsiX0RElimD_lu7Ox-uNllA-fZJzcSm91oJmeYmdnObXfMBm_znJhtgk_PiieDGRM9f6hnxe311bfLD-XN5_cfLy9uSit5PZcVQI9c1ZUw2BM1HXEAWRPkP8WlGVplOyVbJBR9m8_Qdc3QZrFV1nDJz4rz1XeK4ftCadZ7lyyNo_EUlqRRSaxQIUJGX_-D3oUl-rxdpppGtm1dY6ZwpWwMKUUa9BTd3sSDRtDHWPQai86x6GMs-rjEywfnpdtT_0fxO4cMVCuQcstvKf41-j-ur1bRYII22-iSvv1aAdYAyFFwwX8B2qChRA</recordid><startdate>20151101</startdate><enddate>20151101</enddate><creator>Rodrigues, Aline Cristina Ferreira</creator><creator>de Oliveira Moneró, 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potentializes benzo(a)pyrene-induced hepatic cytochrome P450 enzyme activities in Nile tilapia at high concentrations</title><author>Rodrigues, Aline Cristina Ferreira ; de Oliveira Moneró, Tatiana ; Frighetto, Rosa Toyoko Shiraishi ; de Almeida, Eduardo Alves</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-200d137624a1dee8be30056e0376735af97cb7591e14d9d9dfbb8f9c53c7ca353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>17β-Estradiol</topic><topic>Animals</topic><topic>Aquatic ecosystems</topic><topic>Aquatic environment</topic><topic>Aquatic Pollution</topic><topic>Atmospheric Protection/Air Quality Control/Air Pollution</topic><topic>Benzo(a)pyrene</topic><topic>Benzo(a)pyrene - toxicity</topic><topic>Biomarkers</topic><topic>Biomarkers - metabolism</topic><topic>Biotransformation</topic><topic>Biotransformation - drug 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niloticus</topic><topic>Pesticides</topic><topic>polycyclic aromatic hydrocarbons</topic><topic>Pyrene</topic><topic>Synergism</topic><topic>Tilapia</topic><topic>Time Factors</topic><topic>Waste Water Technology</topic><topic>Water Management</topic><topic>Water Pollutants, Chemical - toxicity</topic><topic>Water Pollution Control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodrigues, Aline Cristina Ferreira</creatorcontrib><creatorcontrib>de Oliveira Moneró, Tatiana</creatorcontrib><creatorcontrib>Frighetto, Rosa Toyoko Shiraishi</creatorcontrib><creatorcontrib>de Almeida, Eduardo Alves</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology 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Int</addtitle><date>2015-11-01</date><risdate>2015</risdate><volume>22</volume><issue>22</issue><spage>17367</spage><epage>17374</epage><pages>17367-17374</pages><issn>0944-1344</issn><eissn>1614-7499</eissn><notes>http://dx.doi.org/10.1007/s11356-014-3670-5</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>In the aquatic environment, biotransformation enzymes are established biomarkers for assessing PAH exposure in fish, but little is known about the effect of 17β-estradiol (E2) on these enzymes during exposure to benzo(a)pyrene (BaP). In this study, Nile tilapia (Oreochromis niloticus) were exposed for 3, 5, and 10 days to BaP (300 μg L⁻¹) and E2 (5 μg L⁻¹). These substances were applied isolated or mixed. In the mixture experiment, fish were analyzed pre- and postexposure in order to better understand whether preexposure to the hormone masks the responses activated by PAH or vice versa. Phase I enzymes ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-depenthylase (PROD), and benzyloxyresorufin-O-debenzylase (BROD) activities as well as the phase II enzyme glutathione S-transferase (GST) were analyzed. Isolated E2 treatment decreased EROD activity after 3 days, but this enzyme activity returned to control values after 5 and 10 days of exposure. Isolated BaP treatment significantly induced EROD activity after 3 and 5 days, and the activity returned to control levels after ten exposure days. Combined treatment (E2 + Bap) significantly increased EROD activity, both in the pre- and postexposure. This increase was even higher than in the isolated BaP treatment, suggesting a synergism between these two compounds. When E2 and BaP were used singly, they did not change BROD and PROD activities. However, combined treatment (E2 + Bap) significantly increased PROD activity. Isolated BaP treatment increased GST activity after 10 days. However, this response was not observed in the mixture treatment, suggesting that E2 suppressed the GST induction modulated by BaP. The results put together indicated that E2 altered the biotransformation pathway regarding enzymes activated by BaP in Nile tilapia.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25280508</pmid><doi>10.1007/s11356-014-3670-5</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0944-1344 |
| ispartof | Environmental science and pollution research international, 2015-11, Vol.22 (22), p.17367-17374 |
| issn | 0944-1344 1614-7499 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1751217110 |
| source | ABI/INFORM Global; Springer Link |
| subjects | 17β-Estradiol Animals Aquatic ecosystems Aquatic environment Aquatic Pollution Atmospheric Protection/Air Quality Control/Air Pollution Benzo(a)pyrene Benzo(a)pyrene - toxicity Biomarkers Biomarkers - metabolism Biotransformation Biotransformation - drug effects Chemicals Cichlids - metabolism Combined treatment Cytochrome cytochrome P-450 Cytochrome P450 Dose-Response Relationship, Drug Drug Synergism Earth and Environmental Science Ecotoxicology Environment Environmental Chemistry Environmental Health Enzymatic activity enzyme activity Enzymes estradiol Estradiol - pharmacology Estrogens fish Genes Glutathione transferase Liver - drug effects Liver - enzymology Liver - metabolism Metabolism Metabolites Molecular and Cellular Effects of Contamination in Aquatic Ecosystems Oreochromis niloticus Pesticides polycyclic aromatic hydrocarbons Pyrene Synergism Tilapia Time Factors Waste Water Technology Water Management Water Pollutants, Chemical - toxicity Water Pollution Control |
| title | E2 potentializes benzo(a)pyrene-induced hepatic cytochrome P450 enzyme activities in Nile tilapia at high concentrations |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T19%3A18%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=E2%20potentializes%20benzo(a)pyrene-induced%20hepatic%20cytochrome%20P450%20enzyme%20activities%20in%20Nile%20tilapia%20at%20high%20concentrations&rft.jtitle=Environmental%20science%20and%20pollution%20research%20international&rft.au=Rodrigues,%20Aline%20Cristina%20Ferreira&rft.date=2015-11-01&rft.volume=22&rft.issue=22&rft.spage=17367&rft.epage=17374&rft.pages=17367-17374&rft.issn=0944-1344&rft.eissn=1614-7499&rft_id=info:doi/10.1007/s11356-014-3670-5&rft_dat=%3Cproquest_cross%3E4054980891%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c536t-200d137624a1dee8be30056e0376735af97cb7591e14d9d9dfbb8f9c53c7ca353%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1788599661&rft_id=info:pmid/25280508&rfr_iscdi=true |