The alpha rv beta 6 integrin receptor for Foot-and-mouth disease virus is expressed constitutively on the epithelial cells targeted in cattle

Field strains of Foot-and-mouth disease virus (FMDV) use a number of alpha v-integrins as receptors to initiate infection on cultured cells, and integrins are believed to be the receptors used to target epithelial cells in animals. In this study, immunofluorescence confocal microscopy and real-time...

Full description

Saved in:
Bibliographic Details
Published in:Journal of general virology 2005-10, Vol.86 (10), p.2769-2780
Main Authors: Monaghan, P, Gold, S, Simpson, J, Zhang, Z, Weinreb, PH, Violette, S M, Alexandersen, S, Jackson, T
Format: Article
Language:English
Subjects:
Foot-and-mouth disease virus
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 2780
container_issue 10
container_start_page 2769
container_title Journal of general virology
container_volume 86
creator Monaghan, P
Gold, S
Simpson, J
Zhang, Z
Weinreb, PH
Violette, S M
Alexandersen, S
Jackson, T
description Field strains of Foot-and-mouth disease virus (FMDV) use a number of alpha v-integrins as receptors to initiate infection on cultured cells, and integrins are believed to be the receptors used to target epithelial cells in animals. In this study, immunofluorescence confocal microscopy and real-time RT-PCR were used to investigate expression of two of the integrin receptors of FMDV, alpha v beta 6 and alpha v beta 3, within various epithelia targeted by this virus in cattle. These studies show that alpha v beta 6 is expressed constitutively on the surfaces of epithelial cells at sites where infectious lesions occur during a natural infection, but not at sites where lesions are not normally formed. Expression of alpha v beta 6 protein at these sites showed a good correlation with the relative abundance of beta 6 mRNA. In contrast, alpha v beta 3 protein was only detected at low levels on the vasculature and not on the epithelial cells of any of the tissues investigated. Together, these data suggest that in cattle, alpha v beta 6, rather than alpha v beta 3, serves as the major receptor that determines the tropism of FMDV for the epithelia normally targeted by this virus.
doi_str_mv 10.1099/vir.0.81172-0
format article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_17484279</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17484279</sourcerecordid><originalsourceid>FETCH-proquest_miscellaneous_174842793</originalsourceid><addsrcrecordid>eNqNjT1OAzEQRl2ARPgp6aei28XeLDhbIyIOkD6a7E6yRo5tPOMVHII7YyQOQPHpNe_TU-re6NboYXhcXG51uzHGdo2-UCutu64xa2Ov1DXzu9am75_sSn3vZgL0aUbICxxIEJ7BBaFTdgEyjZQkZjjWbWOUBsPUnGORGSbHhExQS4XBMdBnysRME4wxsDgp4hbyXxADSK1QchXeoYeRvGcQzCeS6tfSiCKebtXlET3T3R9v1MP2dffy1qQcPwqx7M-Of88YKBbeG9tv-s4O63-LP6fRXYg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17484279</pqid></control><display><type>article</type><title>The alpha rv beta 6 integrin receptor for Foot-and-mouth disease virus is expressed constitutively on the epithelial cells targeted in cattle</title><source>Freely Accessible Journals</source><creator>Monaghan, P ; Gold, S ; Simpson, J ; Zhang, Z ; Weinreb, PH ; Violette, S M ; Alexandersen, S ; Jackson, T</creator><creatorcontrib>Monaghan, P ; Gold, S ; Simpson, J ; Zhang, Z ; Weinreb, PH ; Violette, S M ; Alexandersen, S ; Jackson, T</creatorcontrib><description>Field strains of Foot-and-mouth disease virus (FMDV) use a number of alpha v-integrins as receptors to initiate infection on cultured cells, and integrins are believed to be the receptors used to target epithelial cells in animals. In this study, immunofluorescence confocal microscopy and real-time RT-PCR were used to investigate expression of two of the integrin receptors of FMDV, alpha v beta 6 and alpha v beta 3, within various epithelia targeted by this virus in cattle. These studies show that alpha v beta 6 is expressed constitutively on the surfaces of epithelial cells at sites where infectious lesions occur during a natural infection, but not at sites where lesions are not normally formed. Expression of alpha v beta 6 protein at these sites showed a good correlation with the relative abundance of beta 6 mRNA. In contrast, alpha v beta 3 protein was only detected at low levels on the vasculature and not on the epithelial cells of any of the tissues investigated. Together, these data suggest that in cattle, alpha v beta 6, rather than alpha v beta 3, serves as the major receptor that determines the tropism of FMDV for the epithelia normally targeted by this virus.</description><identifier>ISSN: 0022-1317</identifier><identifier>DOI: 10.1099/vir.0.81172-0</identifier><language>eng</language><subject>Foot-and-mouth disease virus</subject><ispartof>Journal of general virology, 2005-10, Vol.86 (10), p.2769-2780</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids></links><search><creatorcontrib>Monaghan, P</creatorcontrib><creatorcontrib>Gold, S</creatorcontrib><creatorcontrib>Simpson, J</creatorcontrib><creatorcontrib>Zhang, Z</creatorcontrib><creatorcontrib>Weinreb, PH</creatorcontrib><creatorcontrib>Violette, S M</creatorcontrib><creatorcontrib>Alexandersen, S</creatorcontrib><creatorcontrib>Jackson, T</creatorcontrib><title>The alpha rv beta 6 integrin receptor for Foot-and-mouth disease virus is expressed constitutively on the epithelial cells targeted in cattle</title><title>Journal of general virology</title><description>Field strains of Foot-and-mouth disease virus (FMDV) use a number of alpha v-integrins as receptors to initiate infection on cultured cells, and integrins are believed to be the receptors used to target epithelial cells in animals. In this study, immunofluorescence confocal microscopy and real-time RT-PCR were used to investigate expression of two of the integrin receptors of FMDV, alpha v beta 6 and alpha v beta 3, within various epithelia targeted by this virus in cattle. These studies show that alpha v beta 6 is expressed constitutively on the surfaces of epithelial cells at sites where infectious lesions occur during a natural infection, but not at sites where lesions are not normally formed. Expression of alpha v beta 6 protein at these sites showed a good correlation with the relative abundance of beta 6 mRNA. In contrast, alpha v beta 3 protein was only detected at low levels on the vasculature and not on the epithelial cells of any of the tissues investigated. Together, these data suggest that in cattle, alpha v beta 6, rather than alpha v beta 3, serves as the major receptor that determines the tropism of FMDV for the epithelia normally targeted by this virus.</description><subject>Foot-and-mouth disease virus</subject><issn>0022-1317</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqNjT1OAzEQRl2ARPgp6aei28XeLDhbIyIOkD6a7E6yRo5tPOMVHII7YyQOQPHpNe_TU-re6NboYXhcXG51uzHGdo2-UCutu64xa2Ov1DXzu9am75_sSn3vZgL0aUbICxxIEJ7BBaFTdgEyjZQkZjjWbWOUBsPUnGORGSbHhExQS4XBMdBnysRME4wxsDgp4hbyXxADSK1QchXeoYeRvGcQzCeS6tfSiCKebtXlET3T3R9v1MP2dffy1qQcPwqx7M-Of88YKBbeG9tv-s4O63-LP6fRXYg</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Monaghan, P</creator><creator>Gold, S</creator><creator>Simpson, J</creator><creator>Zhang, Z</creator><creator>Weinreb, PH</creator><creator>Violette, S M</creator><creator>Alexandersen, S</creator><creator>Jackson, T</creator><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20051001</creationdate><title>The alpha rv beta 6 integrin receptor for Foot-and-mouth disease virus is expressed constitutively on the epithelial cells targeted in cattle</title><author>Monaghan, P ; Gold, S ; Simpson, J ; Zhang, Z ; Weinreb, PH ; Violette, S M ; Alexandersen, S ; Jackson, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_174842793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Foot-and-mouth disease virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Monaghan, P</creatorcontrib><creatorcontrib>Gold, S</creatorcontrib><creatorcontrib>Simpson, J</creatorcontrib><creatorcontrib>Zhang, Z</creatorcontrib><creatorcontrib>Weinreb, PH</creatorcontrib><creatorcontrib>Violette, S M</creatorcontrib><creatorcontrib>Alexandersen, S</creatorcontrib><creatorcontrib>Jackson, T</creatorcontrib><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Monaghan, P</au><au>Gold, S</au><au>Simpson, J</au><au>Zhang, Z</au><au>Weinreb, PH</au><au>Violette, S M</au><au>Alexandersen, S</au><au>Jackson, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The alpha rv beta 6 integrin receptor for Foot-and-mouth disease virus is expressed constitutively on the epithelial cells targeted in cattle</atitle><jtitle>Journal of general virology</jtitle><date>2005-10-01</date><risdate>2005</risdate><volume>86</volume><issue>10</issue><spage>2769</spage><epage>2780</epage><pages>2769-2780</pages><issn>0022-1317</issn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>content type line 23</notes><notes>ObjectType-Feature-2</notes><abstract>Field strains of Foot-and-mouth disease virus (FMDV) use a number of alpha v-integrins as receptors to initiate infection on cultured cells, and integrins are believed to be the receptors used to target epithelial cells in animals. In this study, immunofluorescence confocal microscopy and real-time RT-PCR were used to investigate expression of two of the integrin receptors of FMDV, alpha v beta 6 and alpha v beta 3, within various epithelia targeted by this virus in cattle. These studies show that alpha v beta 6 is expressed constitutively on the surfaces of epithelial cells at sites where infectious lesions occur during a natural infection, but not at sites where lesions are not normally formed. Expression of alpha v beta 6 protein at these sites showed a good correlation with the relative abundance of beta 6 mRNA. In contrast, alpha v beta 3 protein was only detected at low levels on the vasculature and not on the epithelial cells of any of the tissues investigated. Together, these data suggest that in cattle, alpha v beta 6, rather than alpha v beta 3, serves as the major receptor that determines the tropism of FMDV for the epithelia normally targeted by this virus.</abstract><doi>10.1099/vir.0.81172-0</doi></addata></record>
fulltext fulltext
identifier ISSN: 0022-1317
ispartof Journal of general virology, 2005-10, Vol.86 (10), p.2769-2780
issn 0022-1317
language eng
recordid cdi_proquest_miscellaneous_17484279
source Freely Accessible Journals
subjects Foot-and-mouth disease virus
title The alpha rv beta 6 integrin receptor for Foot-and-mouth disease virus is expressed constitutively on the epithelial cells targeted in cattle
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T18%3A35%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20alpha%20rv%20beta%206%20integrin%20receptor%20for%20Foot-and-mouth%20disease%20virus%20is%20expressed%20constitutively%20on%20the%20epithelial%20cells%20targeted%20in%20cattle&rft.jtitle=Journal%20of%20general%20virology&rft.au=Monaghan,%20P&rft.date=2005-10-01&rft.volume=86&rft.issue=10&rft.spage=2769&rft.epage=2780&rft.pages=2769-2780&rft.issn=0022-1317&rft_id=info:doi/10.1099/vir.0.81172-0&rft_dat=%3Cproquest%3E17484279%3C/proquest%3E%3Cgrp_id%3Ecdi_FETCH-proquest_miscellaneous_174842793%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=17484279&rft_id=info:pmid/&rfr_iscdi=true