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Sphere formation of adipose stem cell engineered by poly-2-hydroxyethyl methacrylate induces in vitro angiogenesis through fibroblast growth factor 2
A number of researchers have been reporting a wide range of in vitro and in vivo studies of cell engraftment to enhance angiogenesis using stem cells. Despite these efforts, studies involving three-dimensional (3D) culture method that mimics in vivo environment have not reached its peak yet. In this...
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| Published in: | Biochemical and biophysical research communications 2015-12, Vol.468 (1-2), p.372-379 |
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| cites | cdi_FETCH-LOGICAL-c356t-5cde832baf05452d2c3057399f97848ad105f9ede8190a20b6b95339293fac63 |
| container_end_page | 379 |
| container_issue | 1-2 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Kim, Jong-Ho Lim, I-Rang Joo, Hyung Joon Choi, Seung-Cheol Choi, Ji-Hyun Cui, Long-Hui Im, Lisa Hong, Soon Jun Lim, Do-Sun |
| description | A number of researchers have been reporting a wide range of in vitro and in vivo studies of cell engraftment to enhance angiogenesis using stem cells. Despite these efforts, studies involving three-dimensional (3D) culture method that mimics in vivo environment have not reached its peak yet. In this study, we investigated the change and effects on cellular angiogenic growth factors through sphere formation of adipose stem cell (ASC) which is engineered by poly-2-hydroxyethyl methacrylate (Poly-HEMA). First of all, we successfully induced sphere formation of ASC (sph-ASC) on Poly-HEMA coated plates. sph-ASC represented significantly higher expression levels of anti-apoptotic and hypoxic factors compared to monolayer adherent ASC (adh-ASC). Interestingly, sph-ASC showed higher mRNA levels of the following genes; CD31, CD144, vWF, IGF-2, MCP-1, PDGF-A, VEGF-A, VEGF-C, and FGF-2. In addition, mRNA expressions of angiogenic growth factor receptors such as Flk1, FGFR1, FGFR2, and Tie2 were elevated in sph-ASC. In protein level, Cytokine/Chemokines antibody array revealed a significant increase of FGF-2 in sph-ASC (3.17-fold) compared to adh-ASC. To investigate the effects of FGF-2 on sph-ASC, Matrigel angiogenic invasion assay showed significant reduced level of FGF-2 in FGF-2 siRNA transfected sph-ASC (2.27-fold) compared to negative control siRNA transfected sph-ASC. These findings suggest that Poly-HEMA coated plates induce sphere formation of ASC which has significantly higher expression of FGF-2, and plays a critical role as a major regulating growth factor of in vitro angiogenesis.
•Sphere formation of ASC (sph-ASC) is successfully produced on Poly-HEMA coated plates.•Hypoxia induced by sph-ASC increases expression of angiogenic growth factors.•sph-ASC augments in vitro angiogenic potential via up-regulation of FGF-2.•FGF-2 is a major factor for in vitro angiogenic invasion of sph-ASC. |
| doi_str_mv | 10.1016/j.bbrc.2015.10.083 |
| format | article |
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•Sphere formation of ASC (sph-ASC) is successfully produced on Poly-HEMA coated plates.•Hypoxia induced by sph-ASC increases expression of angiogenic growth factors.•sph-ASC augments in vitro angiogenic potential via up-regulation of FGF-2.•FGF-2 is a major factor for in vitro angiogenic invasion of sph-ASC.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2015.10.083</identifier><identifier>PMID: 26498525</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adipose stem cell ; Adipose Tissue - cytology ; Animals ; Cell Movement ; Cells, Cultured ; Coated Materials, Biocompatible - metabolism ; Fibroblast growth factor 2 ; Fibroblast Growth Factor 2 - metabolism ; Humans ; in vitro angiogenesis ; Mice, Inbred C57BL ; Neovascularization, Physiologic ; Poly-2-hydroxyethyl methacrylate ; Polyhydroxyethyl Methacrylate - metabolism ; Sphere ; Spheroids, Cellular - cytology ; Spheroids, Cellular - metabolism ; Stem Cells - cytology ; Stem Cells - metabolism ; Tissue Engineering</subject><ispartof>Biochemical and biophysical research communications, 2015-12, Vol.468 (1-2), p.372-379</ispartof><rights>2015 Elsevier Inc.</rights><rights>Copyright © 2015 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-5cde832baf05452d2c3057399f97848ad105f9ede8190a20b6b95339293fac63</citedby><cites>FETCH-LOGICAL-c356t-5cde832baf05452d2c3057399f97848ad105f9ede8190a20b6b95339293fac63</cites><orcidid>0000-0001-5751-5177</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,27936,27937</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26498525$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Jong-Ho</creatorcontrib><creatorcontrib>Lim, I-Rang</creatorcontrib><creatorcontrib>Joo, Hyung Joon</creatorcontrib><creatorcontrib>Choi, Seung-Cheol</creatorcontrib><creatorcontrib>Choi, Ji-Hyun</creatorcontrib><creatorcontrib>Cui, Long-Hui</creatorcontrib><creatorcontrib>Im, Lisa</creatorcontrib><creatorcontrib>Hong, Soon Jun</creatorcontrib><creatorcontrib>Lim, Do-Sun</creatorcontrib><title>Sphere formation of adipose stem cell engineered by poly-2-hydroxyethyl methacrylate induces in vitro angiogenesis through fibroblast growth factor 2</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>A number of researchers have been reporting a wide range of in vitro and in vivo studies of cell engraftment to enhance angiogenesis using stem cells. Despite these efforts, studies involving three-dimensional (3D) culture method that mimics in vivo environment have not reached its peak yet. In this study, we investigated the change and effects on cellular angiogenic growth factors through sphere formation of adipose stem cell (ASC) which is engineered by poly-2-hydroxyethyl methacrylate (Poly-HEMA). First of all, we successfully induced sphere formation of ASC (sph-ASC) on Poly-HEMA coated plates. sph-ASC represented significantly higher expression levels of anti-apoptotic and hypoxic factors compared to monolayer adherent ASC (adh-ASC). Interestingly, sph-ASC showed higher mRNA levels of the following genes; CD31, CD144, vWF, IGF-2, MCP-1, PDGF-A, VEGF-A, VEGF-C, and FGF-2. In addition, mRNA expressions of angiogenic growth factor receptors such as Flk1, FGFR1, FGFR2, and Tie2 were elevated in sph-ASC. In protein level, Cytokine/Chemokines antibody array revealed a significant increase of FGF-2 in sph-ASC (3.17-fold) compared to adh-ASC. To investigate the effects of FGF-2 on sph-ASC, Matrigel angiogenic invasion assay showed significant reduced level of FGF-2 in FGF-2 siRNA transfected sph-ASC (2.27-fold) compared to negative control siRNA transfected sph-ASC. These findings suggest that Poly-HEMA coated plates induce sphere formation of ASC which has significantly higher expression of FGF-2, and plays a critical role as a major regulating growth factor of in vitro angiogenesis.
•Sphere formation of ASC (sph-ASC) is successfully produced on Poly-HEMA coated plates.•Hypoxia induced by sph-ASC increases expression of angiogenic growth factors.•sph-ASC augments in vitro angiogenic potential via up-regulation of FGF-2.•FGF-2 is a major factor for in vitro angiogenic invasion of sph-ASC.</description><subject>Adipose stem cell</subject><subject>Adipose Tissue - cytology</subject><subject>Animals</subject><subject>Cell Movement</subject><subject>Cells, Cultured</subject><subject>Coated Materials, Biocompatible - metabolism</subject><subject>Fibroblast growth factor 2</subject><subject>Fibroblast Growth Factor 2 - metabolism</subject><subject>Humans</subject><subject>in vitro angiogenesis</subject><subject>Mice, Inbred C57BL</subject><subject>Neovascularization, Physiologic</subject><subject>Poly-2-hydroxyethyl methacrylate</subject><subject>Polyhydroxyethyl Methacrylate - metabolism</subject><subject>Sphere</subject><subject>Spheroids, Cellular - cytology</subject><subject>Spheroids, Cellular - metabolism</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Tissue Engineering</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNp9kc1u1DAUhS0EokPhBVggL9lk8E-ciSU2qIKCVIkFXbCz_HMz8SiJg-205EVY8yw8WR1NYcnqSEffPVf3HoReU7KnhDbvTntjot0zQkUx9qTlT9COEkkqRkn9FO0IIU3FJP1-gV6kdCKE0rqRz9EFa2rZCiZ26Ne3uYcIuAtx1NmHCYcOa-fnkACnDCO2MAwYpqOfoIAOmxXPYVgrVvWri-HnCrlfBzwW0Taug86A_eQWC6non993PseAdQkIR5gg-YRzH8Ny7HHnTQxm0CnjYwz3uTja5hAxe4medXpI8OpRL9Htp4-3V5-rm6_XX64-3FSWiyZXwjpoOTO6I6IWzDHLiThwKTt5aOtWO0pEJ6FAVBLNiGmMFJxLJnnZ1PBL9PYcO8fwY4GU1ejTdrCeICxJ0QNvairqmhSUnVEbQ0oROjVHP-q4KkrUVoc6qa0OtdWxeaWOMvTmMX8xI7h_I3__X4D3ZwDKkXceokrWw2TB-Qg2Kxf8__IfAEVln9w</recordid><startdate>20151204</startdate><enddate>20151204</enddate><creator>Kim, Jong-Ho</creator><creator>Lim, I-Rang</creator><creator>Joo, Hyung Joon</creator><creator>Choi, Seung-Cheol</creator><creator>Choi, Ji-Hyun</creator><creator>Cui, Long-Hui</creator><creator>Im, Lisa</creator><creator>Hong, Soon Jun</creator><creator>Lim, Do-Sun</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5751-5177</orcidid></search><sort><creationdate>20151204</creationdate><title>Sphere formation of adipose stem cell engineered by poly-2-hydroxyethyl methacrylate induces in vitro angiogenesis through fibroblast growth factor 2</title><author>Kim, Jong-Ho ; Lim, I-Rang ; Joo, Hyung Joon ; Choi, Seung-Cheol ; Choi, Ji-Hyun ; Cui, Long-Hui ; Im, Lisa ; Hong, Soon Jun ; Lim, Do-Sun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-5cde832baf05452d2c3057399f97848ad105f9ede8190a20b6b95339293fac63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adipose stem cell</topic><topic>Adipose Tissue - cytology</topic><topic>Animals</topic><topic>Cell Movement</topic><topic>Cells, Cultured</topic><topic>Coated Materials, Biocompatible - metabolism</topic><topic>Fibroblast growth factor 2</topic><topic>Fibroblast Growth Factor 2 - metabolism</topic><topic>Humans</topic><topic>in vitro angiogenesis</topic><topic>Mice, Inbred C57BL</topic><topic>Neovascularization, Physiologic</topic><topic>Poly-2-hydroxyethyl methacrylate</topic><topic>Polyhydroxyethyl Methacrylate - metabolism</topic><topic>Sphere</topic><topic>Spheroids, Cellular - cytology</topic><topic>Spheroids, Cellular - metabolism</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><topic>Tissue Engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Jong-Ho</creatorcontrib><creatorcontrib>Lim, I-Rang</creatorcontrib><creatorcontrib>Joo, Hyung Joon</creatorcontrib><creatorcontrib>Choi, Seung-Cheol</creatorcontrib><creatorcontrib>Choi, Ji-Hyun</creatorcontrib><creatorcontrib>Cui, Long-Hui</creatorcontrib><creatorcontrib>Im, Lisa</creatorcontrib><creatorcontrib>Hong, Soon Jun</creatorcontrib><creatorcontrib>Lim, Do-Sun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Jong-Ho</au><au>Lim, I-Rang</au><au>Joo, Hyung Joon</au><au>Choi, Seung-Cheol</au><au>Choi, Ji-Hyun</au><au>Cui, Long-Hui</au><au>Im, Lisa</au><au>Hong, Soon Jun</au><au>Lim, Do-Sun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sphere formation of adipose stem cell engineered by poly-2-hydroxyethyl methacrylate induces in vitro angiogenesis through fibroblast growth factor 2</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2015-12-04</date><risdate>2015</risdate><volume>468</volume><issue>1-2</issue><spage>372</spage><epage>379</epage><pages>372-379</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>A number of researchers have been reporting a wide range of in vitro and in vivo studies of cell engraftment to enhance angiogenesis using stem cells. Despite these efforts, studies involving three-dimensional (3D) culture method that mimics in vivo environment have not reached its peak yet. In this study, we investigated the change and effects on cellular angiogenic growth factors through sphere formation of adipose stem cell (ASC) which is engineered by poly-2-hydroxyethyl methacrylate (Poly-HEMA). First of all, we successfully induced sphere formation of ASC (sph-ASC) on Poly-HEMA coated plates. sph-ASC represented significantly higher expression levels of anti-apoptotic and hypoxic factors compared to monolayer adherent ASC (adh-ASC). Interestingly, sph-ASC showed higher mRNA levels of the following genes; CD31, CD144, vWF, IGF-2, MCP-1, PDGF-A, VEGF-A, VEGF-C, and FGF-2. In addition, mRNA expressions of angiogenic growth factor receptors such as Flk1, FGFR1, FGFR2, and Tie2 were elevated in sph-ASC. In protein level, Cytokine/Chemokines antibody array revealed a significant increase of FGF-2 in sph-ASC (3.17-fold) compared to adh-ASC. To investigate the effects of FGF-2 on sph-ASC, Matrigel angiogenic invasion assay showed significant reduced level of FGF-2 in FGF-2 siRNA transfected sph-ASC (2.27-fold) compared to negative control siRNA transfected sph-ASC. These findings suggest that Poly-HEMA coated plates induce sphere formation of ASC which has significantly higher expression of FGF-2, and plays a critical role as a major regulating growth factor of in vitro angiogenesis.
•Sphere formation of ASC (sph-ASC) is successfully produced on Poly-HEMA coated plates.•Hypoxia induced by sph-ASC increases expression of angiogenic growth factors.•sph-ASC augments in vitro angiogenic potential via up-regulation of FGF-2.•FGF-2 is a major factor for in vitro angiogenic invasion of sph-ASC.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>26498525</pmid><doi>10.1016/j.bbrc.2015.10.083</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-5751-5177</orcidid></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2015-12, Vol.468 (1-2), p.372-379 |
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| subjects | Adipose stem cell Adipose Tissue - cytology Animals Cell Movement Cells, Cultured Coated Materials, Biocompatible - metabolism Fibroblast growth factor 2 Fibroblast Growth Factor 2 - metabolism Humans in vitro angiogenesis Mice, Inbred C57BL Neovascularization, Physiologic Poly-2-hydroxyethyl methacrylate Polyhydroxyethyl Methacrylate - metabolism Sphere Spheroids, Cellular - cytology Spheroids, Cellular - metabolism Stem Cells - cytology Stem Cells - metabolism Tissue Engineering |
| title | Sphere formation of adipose stem cell engineered by poly-2-hydroxyethyl methacrylate induces in vitro angiogenesis through fibroblast growth factor 2 |
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