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Genotoxic testing of titanium dioxide anatase nanoparticles using the wing-spot test and the comet assay in Drosophila
•The genotoxicity of TiO2 NPs and their bulk form was evaluated in Drosophila.•There is a lack of mutagenicity for both TiO2 forms evaluated in the wing-spot test.•Only TiO2 NPs show DNA damage in Drosophila hemocytes measured by the comet assay.•Using more than one genetic-endpoint is suitable for...
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| Published in: | Mutation research. Genetic toxicology and environmental mutagenesis 2015-01, Vol.778, p.12-21 |
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| container_title | Mutation research. Genetic toxicology and environmental mutagenesis |
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| creator | Carmona, Erico R. Escobar, Bibi Vales, Gerard Marcos, Ricard |
| description | •The genotoxicity of TiO2 NPs and their bulk form was evaluated in Drosophila.•There is a lack of mutagenicity for both TiO2 forms evaluated in the wing-spot test.•Only TiO2 NPs show DNA damage in Drosophila hemocytes measured by the comet assay.•Using more than one genetic-endpoint is suitable for genotoxic testing of TiO2 NPs.
Titanium dioxide nanoparticles (TiO2 NPs) are widely used for preparations of sunscreens, cosmetics, food and personal care products. However, the possible genotoxic risk associated with this nano-scale material exposure is not clear, especially in whole organisms. In the present study, we explored the in vivo genotoxic activity of TiO2 NPs as well as their TiO2 bulk form using two well-established genotoxic assays, the wing spot test and the comet assay in Drosophila melanogaster. To determine the extent of tissue damage induced by TiO2 NPs in Drosophila larvae, the trypan blue dye exclusion test was also applied. Both compounds were supplied to third instar larvae by ingestion at concentration ranging from 0.08 to 1.60mg/mL. The results obtained in the present study indicate that TiO2 NPs can reach and induce cytotoxic effects on midgut and imaginal disc tissues of larvae, but they do not promote genotoxicity in the wing-spot test of Drosophila. However, when both nano- and large-size forms of TiO2 were evaluated with the comet assay in Drosophila hemocytes, a significant increase in DNA damage, with a direct dose-response pattern, was observed for TiO2 NPs. The results obtained with the comet assay suggest that the primary DNA damage associated with TiO2 NPs exposure in Drosophila could be associated with specific physico-chemical properties of nano-TiO2, since no effects were observed with the bulk form. This study remarks the usefulness of using more than one genetic end-point in the evaluation of the genotoxic potential of nanomaterials. |
| doi_str_mv | 10.1016/j.mrgentox.2014.12.004 |
| format | article |
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Titanium dioxide nanoparticles (TiO2 NPs) are widely used for preparations of sunscreens, cosmetics, food and personal care products. However, the possible genotoxic risk associated with this nano-scale material exposure is not clear, especially in whole organisms. In the present study, we explored the in vivo genotoxic activity of TiO2 NPs as well as their TiO2 bulk form using two well-established genotoxic assays, the wing spot test and the comet assay in Drosophila melanogaster. To determine the extent of tissue damage induced by TiO2 NPs in Drosophila larvae, the trypan blue dye exclusion test was also applied. Both compounds were supplied to third instar larvae by ingestion at concentration ranging from 0.08 to 1.60mg/mL. The results obtained in the present study indicate that TiO2 NPs can reach and induce cytotoxic effects on midgut and imaginal disc tissues of larvae, but they do not promote genotoxicity in the wing-spot test of Drosophila. However, when both nano- and large-size forms of TiO2 were evaluated with the comet assay in Drosophila hemocytes, a significant increase in DNA damage, with a direct dose-response pattern, was observed for TiO2 NPs. The results obtained with the comet assay suggest that the primary DNA damage associated with TiO2 NPs exposure in Drosophila could be associated with specific physico-chemical properties of nano-TiO2, since no effects were observed with the bulk form. This study remarks the usefulness of using more than one genetic end-point in the evaluation of the genotoxic potential of nanomaterials.</description><identifier>ISSN: 1383-5718</identifier><identifier>EISSN: 1879-3592</identifier><identifier>DOI: 10.1016/j.mrgentox.2014.12.004</identifier><identifier>PMID: 25726144</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Bioassays ; Comet Assay ; Cytotoxicity ; Cytotoxins - toxicity ; DNA Damage ; Drosophila melanogaster ; Drosophila melanogaster - cytology ; Drosophila melanogaster - drug effects ; Drosophila melanogaster - genetics ; Drosophila melanogaster - metabolism ; Hemocytes - cytology ; Hemocytes - drug effects ; Hemocytes - metabolism ; Insects ; Larva - cytology ; Larva - drug effects ; Larva - genetics ; Larva - metabolism ; Metal Nanoparticles - toxicity ; Mutagenicity Tests ; Nanomaterials ; Nanoparticles ; Physical chemistry ; TiO2 ; Titanium - chemistry ; Titanium - toxicity ; Titanium oxide powders ; Trypan Blue ; Wings, Animal - drug effects</subject><ispartof>Mutation research. Genetic toxicology and environmental mutagenesis, 2015-01, Vol.778, p.12-21</ispartof><rights>2014 Elsevier B.V.</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Jan 15, 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-3e7ace2cb1432924709240b7d701a29a415edd4d19a8f82e131f260451ea43ed3</citedby><cites>FETCH-LOGICAL-c429t-3e7ace2cb1432924709240b7d701a29a415edd4d19a8f82e131f260451ea43ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25726144$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carmona, Erico R.</creatorcontrib><creatorcontrib>Escobar, Bibi</creatorcontrib><creatorcontrib>Vales, Gerard</creatorcontrib><creatorcontrib>Marcos, Ricard</creatorcontrib><title>Genotoxic testing of titanium dioxide anatase nanoparticles using the wing-spot test and the comet assay in Drosophila</title><title>Mutation research. Genetic toxicology and environmental mutagenesis</title><addtitle>Mutat Res Genet Toxicol Environ Mutagen</addtitle><description>•The genotoxicity of TiO2 NPs and their bulk form was evaluated in Drosophila.•There is a lack of mutagenicity for both TiO2 forms evaluated in the wing-spot test.•Only TiO2 NPs show DNA damage in Drosophila hemocytes measured by the comet assay.•Using more than one genetic-endpoint is suitable for genotoxic testing of TiO2 NPs.
Titanium dioxide nanoparticles (TiO2 NPs) are widely used for preparations of sunscreens, cosmetics, food and personal care products. However, the possible genotoxic risk associated with this nano-scale material exposure is not clear, especially in whole organisms. In the present study, we explored the in vivo genotoxic activity of TiO2 NPs as well as their TiO2 bulk form using two well-established genotoxic assays, the wing spot test and the comet assay in Drosophila melanogaster. To determine the extent of tissue damage induced by TiO2 NPs in Drosophila larvae, the trypan blue dye exclusion test was also applied. Both compounds were supplied to third instar larvae by ingestion at concentration ranging from 0.08 to 1.60mg/mL. The results obtained in the present study indicate that TiO2 NPs can reach and induce cytotoxic effects on midgut and imaginal disc tissues of larvae, but they do not promote genotoxicity in the wing-spot test of Drosophila. However, when both nano- and large-size forms of TiO2 were evaluated with the comet assay in Drosophila hemocytes, a significant increase in DNA damage, with a direct dose-response pattern, was observed for TiO2 NPs. The results obtained with the comet assay suggest that the primary DNA damage associated with TiO2 NPs exposure in Drosophila could be associated with specific physico-chemical properties of nano-TiO2, since no effects were observed with the bulk form. This study remarks the usefulness of using more than one genetic end-point in the evaluation of the genotoxic potential of nanomaterials.</description><subject>Animals</subject><subject>Bioassays</subject><subject>Comet Assay</subject><subject>Cytotoxicity</subject><subject>Cytotoxins - toxicity</subject><subject>DNA Damage</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - cytology</subject><subject>Drosophila melanogaster - drug effects</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila melanogaster - metabolism</subject><subject>Hemocytes - cytology</subject><subject>Hemocytes - drug effects</subject><subject>Hemocytes - metabolism</subject><subject>Insects</subject><subject>Larva - cytology</subject><subject>Larva - drug effects</subject><subject>Larva - genetics</subject><subject>Larva - metabolism</subject><subject>Metal Nanoparticles - toxicity</subject><subject>Mutagenicity Tests</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Physical chemistry</subject><subject>TiO2</subject><subject>Titanium - chemistry</subject><subject>Titanium - toxicity</subject><subject>Titanium oxide powders</subject><subject>Trypan Blue</subject><subject>Wings, Animal - drug effects</subject><issn>1383-5718</issn><issn>1879-3592</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqFkcFu3CAQhq2qUZOmfYUIqZde7DCAjX1rlbRppEi5tGfEwjhhZYMLOG3ePuxu0kMvucAwfPMzw19VZ0AboNCdb5s53qHP4W_DKIgGWEOpeFOdQC-HmrcDe1ti3vO6ldAfV-9T2lLKKKf9u-qYtZJ1IMRJ9XCFPhQVZ0jGlJ2_I2Ek2WXt3ToT68qVRaK9zjoh8dqHRcfszISJrGnH53skf0pQpyXkvUrB7T5twozllJJ-JM6TyxhSWO7dpD9UR6OeEn583k-rX9-__bz4Ud_cXl1ffL2pjWBDrjlKbZCZDQjOBiYkLQvdSCspaDZoAS1aKywMuh97hsBhZB0VLaAWHC0_rT4fdJcYfq-lNTW7ZHCatMewJgWSyU7KnsnX0a4Ic9lBX9BP_6HbsEZfBtlTAEPL20J1B8qUsVPEUS3RzTo-KqBqZ6LaqhcT1c5EBUwVE0vh2bP8upnR_it7ca0AXw4Alq97cBhVMg69QesimqxscK-98QSjILHA</recordid><startdate>20150115</startdate><enddate>20150115</enddate><creator>Carmona, Erico R.</creator><creator>Escobar, Bibi</creator><creator>Vales, Gerard</creator><creator>Marcos, Ricard</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>7SS</scope></search><sort><creationdate>20150115</creationdate><title>Genotoxic testing of titanium dioxide anatase nanoparticles using the wing-spot test and the comet assay in Drosophila</title><author>Carmona, Erico R. ; Escobar, Bibi ; Vales, Gerard ; Marcos, Ricard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-3e7ace2cb1432924709240b7d701a29a415edd4d19a8f82e131f260451ea43ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Bioassays</topic><topic>Comet Assay</topic><topic>Cytotoxicity</topic><topic>Cytotoxins - toxicity</topic><topic>DNA Damage</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - cytology</topic><topic>Drosophila melanogaster - drug effects</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila melanogaster - metabolism</topic><topic>Hemocytes - cytology</topic><topic>Hemocytes - drug effects</topic><topic>Hemocytes - metabolism</topic><topic>Insects</topic><topic>Larva - cytology</topic><topic>Larva - drug effects</topic><topic>Larva - genetics</topic><topic>Larva - metabolism</topic><topic>Metal Nanoparticles - toxicity</topic><topic>Mutagenicity Tests</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Physical chemistry</topic><topic>TiO2</topic><topic>Titanium - chemistry</topic><topic>Titanium - toxicity</topic><topic>Titanium oxide powders</topic><topic>Trypan Blue</topic><topic>Wings, Animal - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carmona, Erico R.</creatorcontrib><creatorcontrib>Escobar, Bibi</creatorcontrib><creatorcontrib>Vales, Gerard</creatorcontrib><creatorcontrib>Marcos, Ricard</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Entomology Abstracts (Full archive)</collection><jtitle>Mutation research. Genetic toxicology and environmental mutagenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carmona, Erico R.</au><au>Escobar, Bibi</au><au>Vales, Gerard</au><au>Marcos, Ricard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotoxic testing of titanium dioxide anatase nanoparticles using the wing-spot test and the comet assay in Drosophila</atitle><jtitle>Mutation research. Genetic toxicology and environmental mutagenesis</jtitle><addtitle>Mutat Res Genet Toxicol Environ Mutagen</addtitle><date>2015-01-15</date><risdate>2015</risdate><volume>778</volume><spage>12</spage><epage>21</epage><pages>12-21</pages><issn>1383-5718</issn><eissn>1879-3592</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>•The genotoxicity of TiO2 NPs and their bulk form was evaluated in Drosophila.•There is a lack of mutagenicity for both TiO2 forms evaluated in the wing-spot test.•Only TiO2 NPs show DNA damage in Drosophila hemocytes measured by the comet assay.•Using more than one genetic-endpoint is suitable for genotoxic testing of TiO2 NPs.
Titanium dioxide nanoparticles (TiO2 NPs) are widely used for preparations of sunscreens, cosmetics, food and personal care products. However, the possible genotoxic risk associated with this nano-scale material exposure is not clear, especially in whole organisms. In the present study, we explored the in vivo genotoxic activity of TiO2 NPs as well as their TiO2 bulk form using two well-established genotoxic assays, the wing spot test and the comet assay in Drosophila melanogaster. To determine the extent of tissue damage induced by TiO2 NPs in Drosophila larvae, the trypan blue dye exclusion test was also applied. Both compounds were supplied to third instar larvae by ingestion at concentration ranging from 0.08 to 1.60mg/mL. The results obtained in the present study indicate that TiO2 NPs can reach and induce cytotoxic effects on midgut and imaginal disc tissues of larvae, but they do not promote genotoxicity in the wing-spot test of Drosophila. However, when both nano- and large-size forms of TiO2 were evaluated with the comet assay in Drosophila hemocytes, a significant increase in DNA damage, with a direct dose-response pattern, was observed for TiO2 NPs. The results obtained with the comet assay suggest that the primary DNA damage associated with TiO2 NPs exposure in Drosophila could be associated with specific physico-chemical properties of nano-TiO2, since no effects were observed with the bulk form. This study remarks the usefulness of using more than one genetic end-point in the evaluation of the genotoxic potential of nanomaterials.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25726144</pmid><doi>10.1016/j.mrgentox.2014.12.004</doi><tpages>10</tpages></addata></record> |
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| ispartof | Mutation research. Genetic toxicology and environmental mutagenesis, 2015-01, Vol.778, p.12-21 |
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| source | ScienceDirect Journals |
| subjects | Animals Bioassays Comet Assay Cytotoxicity Cytotoxins - toxicity DNA Damage Drosophila melanogaster Drosophila melanogaster - cytology Drosophila melanogaster - drug effects Drosophila melanogaster - genetics Drosophila melanogaster - metabolism Hemocytes - cytology Hemocytes - drug effects Hemocytes - metabolism Insects Larva - cytology Larva - drug effects Larva - genetics Larva - metabolism Metal Nanoparticles - toxicity Mutagenicity Tests Nanomaterials Nanoparticles Physical chemistry TiO2 Titanium - chemistry Titanium - toxicity Titanium oxide powders Trypan Blue Wings, Animal - drug effects |
| title | Genotoxic testing of titanium dioxide anatase nanoparticles using the wing-spot test and the comet assay in Drosophila |
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