Splenic cultures from rainbow trout,Oncorhynchus mykiss: establishment and characterisation

This study describes the culture conditions and the phenotypic features of different types of splenic cultures established from explants. Using the same culture technique it was possible to grow splenic explants from which monolayers of reticular origin, long-term haematopoietic cultures, and subcul...

Full description

Saved in:
Bibliographic Details
Published in:Fish & shellfish immunology 1998-11, Vol.8 (8), p.589-606
Main Authors: Flaño, E., López-Fierro, P., Álvarez, F., Razquin, B., Villena, A.
Format: Article
Language:English
Subjects:
Oncorhynchus mykiss
spleen, tissue culture, fish, haematopoiesis,Oncorhynchus mykiss
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study describes the culture conditions and the phenotypic features of different types of splenic cultures established from explants. Using the same culture technique it was possible to grow splenic explants from which monolayers of reticular origin, long-term haematopoietic cultures, and subcultures were obtained. The cultures were characterised by light and electron microscopy, cytochemical and immuno-cytochemical analyses, phagocytic activity and susceptibility to virus. The cultures comprised multilayers of epithelioid and fibroblastoid cells with haemopoietic foci, melanomacrophages and eosinophilic granular cells. The cytochemical and immuno-cytochemical analyses revealed that the stromal cells were always positive for ANAE activity. The stromal cells in primary cultures were negatively or weakly stained by antibodies directed against cytokeratins and S-100, but in the subcultures they were strongly stained by these antibodies. The stromal cells had very poor phagocytic activity and were susceptible to VHS virus.
ISSN:1050-4648
1095-9947
DOI:10.1006/fsim.1998.0165