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Application of an aqueous two-phase micellar system to extract bromelain from pineapple (Ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations
Bromelain is a set of proteolytic enzymes found in pineapple (Ananas comosus) tissues such as stem, fruit and leaves. Because of its proteolytic activity, bromelain has potential applications in the cosmetic, pharmaceutical, and food industries. The present study focused on the recovery of bromelain...
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Published in: | Biotechnology progress 2015-07, Vol.31 (4), p.937-945 |
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creator | Spir, Lívia Genovez Ataide, Janaína Artem De Lencastre Novaes, Letícia Celia Moriel, Patrícia Mazzola, Priscila Gava De Borba Gurpilhares, Daniela Silveira, Edgar Pessoa Jr, Adalberto Tambourgi, Elias Basile |
description | Bromelain is a set of proteolytic enzymes found in pineapple (Ananas comosus) tissues such as stem, fruit and leaves. Because of its proteolytic activity, bromelain has potential applications in the cosmetic, pharmaceutical, and food industries. The present study focused on the recovery of bromelain from pineapple peel by liquid–liquid extraction in aqueous two‐phase micellar systems (ATPMS), using Triton X‐114 (TX‐114) and McIlvaine buffer, in the absence and presence of electrolytes CaCl2 and KI; the cloud points of the generated extraction systems were studied by plotting binodal curves. Based on the cloud points, three temperatures were selected for extraction: 30, 33, and 36°C for systems in the absence of salts; 40, 43, and 46°C in the presence of KI; 24, 27, and 30°C in the presence of CaCl2. Total protein and enzymatic activities were analyzed to monitor bromelain. Employing the ATPMS chosen for extraction (0.5 M KI with 3% TX‐114, at pH 6.0, at 40°C), the bromelain extract stability was assessed after incorporation into three cosmetic bases: an anhydrous gel, a cream, and a cream‐gel formulation. The cream‐gel formulation presented as the most appropriate base to convey bromelain, and its optimal storage conditions were found to be 4.0 ± 0.5°C. The selected ATPMS enabled the extraction of a biomolecule with high added value from waste lined‐up in a cosmetic formulation, allowing for exploration of further cosmetic potential. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 31:937–945, 2015 |
doi_str_mv | 10.1002/btpr.2098 |
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Because of its proteolytic activity, bromelain has potential applications in the cosmetic, pharmaceutical, and food industries. The present study focused on the recovery of bromelain from pineapple peel by liquid–liquid extraction in aqueous two‐phase micellar systems (ATPMS), using Triton X‐114 (TX‐114) and McIlvaine buffer, in the absence and presence of electrolytes CaCl2 and KI; the cloud points of the generated extraction systems were studied by plotting binodal curves. Based on the cloud points, three temperatures were selected for extraction: 30, 33, and 36°C for systems in the absence of salts; 40, 43, and 46°C in the presence of KI; 24, 27, and 30°C in the presence of CaCl2. Total protein and enzymatic activities were analyzed to monitor bromelain. Employing the ATPMS chosen for extraction (0.5 M KI with 3% TX‐114, at pH 6.0, at 40°C), the bromelain extract stability was assessed after incorporation into three cosmetic bases: an anhydrous gel, a cream, and a cream‐gel formulation. The cream‐gel formulation presented as the most appropriate base to convey bromelain, and its optimal storage conditions were found to be 4.0 ± 0.5°C. The selected ATPMS enabled the extraction of a biomolecule with high added value from waste lined‐up in a cosmetic formulation, allowing for exploration of further cosmetic potential. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 31:937–945, 2015</description><identifier>ISSN: 8756-7938</identifier><identifier>EISSN: 1520-6033</identifier><identifier>DOI: 10.1002/btpr.2098</identifier><identifier>PMID: 25919128</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Ananas - enzymology ; Ananas comosus ; Biotechnology ; bromelain ; Bromelains - chemistry ; Bromelains - isolation & purification ; Chemical Fractionation - methods ; cosmetic stability ; Cosmetics - chemistry ; enzyme purification ; liquid-liquid extraction ; Micelles ; protein partition</subject><ispartof>Biotechnology progress, 2015-07, Vol.31 (4), p.937-945</ispartof><rights>2015 American Institute of Chemical Engineers</rights><rights>2015 American Institute of Chemical Engineers.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4618-f9cf4ff1d134ba0abedc2ad9300bac57281339b0078d75facac2dff27e20fc003</citedby><cites>FETCH-LOGICAL-c4618-f9cf4ff1d134ba0abedc2ad9300bac57281339b0078d75facac2dff27e20fc003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbtpr.2098$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbtpr.2098$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25919128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Spir, Lívia Genovez</creatorcontrib><creatorcontrib>Ataide, Janaína Artem</creatorcontrib><creatorcontrib>De Lencastre Novaes, Letícia Celia</creatorcontrib><creatorcontrib>Moriel, Patrícia</creatorcontrib><creatorcontrib>Mazzola, Priscila Gava</creatorcontrib><creatorcontrib>De Borba Gurpilhares, Daniela</creatorcontrib><creatorcontrib>Silveira, Edgar</creatorcontrib><creatorcontrib>Pessoa Jr, Adalberto</creatorcontrib><creatorcontrib>Tambourgi, Elias Basile</creatorcontrib><title>Application of an aqueous two-phase micellar system to extract bromelain from pineapple (Ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations</title><title>Biotechnology progress</title><addtitle>Biotechnol Progress</addtitle><description>Bromelain is a set of proteolytic enzymes found in pineapple (Ananas comosus) tissues such as stem, fruit and leaves. Because of its proteolytic activity, bromelain has potential applications in the cosmetic, pharmaceutical, and food industries. The present study focused on the recovery of bromelain from pineapple peel by liquid–liquid extraction in aqueous two‐phase micellar systems (ATPMS), using Triton X‐114 (TX‐114) and McIlvaine buffer, in the absence and presence of electrolytes CaCl2 and KI; the cloud points of the generated extraction systems were studied by plotting binodal curves. Based on the cloud points, three temperatures were selected for extraction: 30, 33, and 36°C for systems in the absence of salts; 40, 43, and 46°C in the presence of KI; 24, 27, and 30°C in the presence of CaCl2. Total protein and enzymatic activities were analyzed to monitor bromelain. Employing the ATPMS chosen for extraction (0.5 M KI with 3% TX‐114, at pH 6.0, at 40°C), the bromelain extract stability was assessed after incorporation into three cosmetic bases: an anhydrous gel, a cream, and a cream‐gel formulation. The cream‐gel formulation presented as the most appropriate base to convey bromelain, and its optimal storage conditions were found to be 4.0 ± 0.5°C. The selected ATPMS enabled the extraction of a biomolecule with high added value from waste lined‐up in a cosmetic formulation, allowing for exploration of further cosmetic potential. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 31:937–945, 2015</description><subject>Ananas - enzymology</subject><subject>Ananas comosus</subject><subject>Biotechnology</subject><subject>bromelain</subject><subject>Bromelains - chemistry</subject><subject>Bromelains - isolation & purification</subject><subject>Chemical Fractionation - methods</subject><subject>cosmetic stability</subject><subject>Cosmetics - chemistry</subject><subject>enzyme purification</subject><subject>liquid-liquid extraction</subject><subject>Micelles</subject><subject>protein partition</subject><issn>8756-7938</issn><issn>1520-6033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkVtrFDEYhoModrt64R-QgDftxbQ57Jwu16JdpahoPdyFbzIJpmYmY5JhO_-qP9HM7lpBELwISeDJ84X3RegZJWeUEHbexMGfMVJXD9CC5oxkBeH8IVpUZV5kZc2rI3Qcwg0hpCIFe4yOWF7TmrJqge7Ww2CNhGhcj53G0GP4OSo3Bhy3Lhu-Q1C4M1JZCx6HKUTV4eiwuo0eZMSNd52yYHqs0wkPpleQjAqfrHvoIWDpOhfGcIoHpSzeQhKkIW1aYKdgwjz0jyREaIw1ccLpIl3oVDQSa-e70e7-GJ6gRxpsUE8P-xJ9fv3q-mKTXb2_fHOxvsrkqqBVpmupV1rTlvJVAwQa1UoGbc0JaUDmJaso53VDSFm1Za5BgmSt1qxUjGhJCF-ik7138C4FEqLoTNjF0M_pCFqSmpZF8X8oL-oq5Z3QF3-hN270KYodlYB8_tcSne4p6V0IXmkxeNOBnwQlYm5czI2LufHEPj8Yx6ZT7T35u-IEnO-BrbFq-rdJvLz-8PGgzPYvTCrr9v4F-B-iKHmZi6_vLsWXDf30Nq--iQ3_BQdLyU4</recordid><startdate>201507</startdate><enddate>201507</enddate><creator>Spir, Lívia Genovez</creator><creator>Ataide, Janaína Artem</creator><creator>De Lencastre Novaes, Letícia Celia</creator><creator>Moriel, Patrícia</creator><creator>Mazzola, Priscila Gava</creator><creator>De Borba Gurpilhares, Daniela</creator><creator>Silveira, Edgar</creator><creator>Pessoa Jr, Adalberto</creator><creator>Tambourgi, Elias Basile</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201507</creationdate><title>Application of an aqueous two-phase micellar system to extract bromelain from pineapple (Ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations</title><author>Spir, Lívia Genovez ; Ataide, Janaína Artem ; De Lencastre Novaes, Letícia Celia ; Moriel, Patrícia ; Mazzola, Priscila Gava ; De Borba Gurpilhares, Daniela ; Silveira, Edgar ; Pessoa Jr, Adalberto ; Tambourgi, Elias Basile</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4618-f9cf4ff1d134ba0abedc2ad9300bac57281339b0078d75facac2dff27e20fc003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Ananas - enzymology</topic><topic>Ananas comosus</topic><topic>Biotechnology</topic><topic>bromelain</topic><topic>Bromelains - chemistry</topic><topic>Bromelains - isolation & purification</topic><topic>Chemical Fractionation - methods</topic><topic>cosmetic stability</topic><topic>Cosmetics - chemistry</topic><topic>enzyme purification</topic><topic>liquid-liquid extraction</topic><topic>Micelles</topic><topic>protein partition</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Spir, Lívia Genovez</creatorcontrib><creatorcontrib>Ataide, Janaína Artem</creatorcontrib><creatorcontrib>De Lencastre Novaes, Letícia Celia</creatorcontrib><creatorcontrib>Moriel, Patrícia</creatorcontrib><creatorcontrib>Mazzola, Priscila Gava</creatorcontrib><creatorcontrib>De Borba Gurpilhares, Daniela</creatorcontrib><creatorcontrib>Silveira, Edgar</creatorcontrib><creatorcontrib>Pessoa Jr, Adalberto</creatorcontrib><creatorcontrib>Tambourgi, Elias Basile</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology progress</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Spir, Lívia Genovez</au><au>Ataide, Janaína Artem</au><au>De Lencastre Novaes, Letícia Celia</au><au>Moriel, Patrícia</au><au>Mazzola, Priscila Gava</au><au>De Borba Gurpilhares, Daniela</au><au>Silveira, Edgar</au><au>Pessoa Jr, Adalberto</au><au>Tambourgi, Elias Basile</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of an aqueous two-phase micellar system to extract bromelain from pineapple (Ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations</atitle><jtitle>Biotechnology progress</jtitle><addtitle>Biotechnol Progress</addtitle><date>2015-07</date><risdate>2015</risdate><volume>31</volume><issue>4</issue><spage>937</spage><epage>945</epage><pages>937-945</pages><issn>8756-7938</issn><eissn>1520-6033</eissn><notes>ArticleID:BTPR2098</notes><notes>istex:9FE0A2B8E894B5F6B504F593E6AE3B4F7A025574</notes><notes>ark:/67375/WNG-VH1SJ58X-H</notes><notes>Sao Paulo Research Foundation (FAPESP)</notes><notes>This article was published online on 15 May 2015. An error was subsequently identified. This notice is included in the online publication to indicate that it was corrected on 04 March 2016.</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Bromelain is a set of proteolytic enzymes found in pineapple (Ananas comosus) tissues such as stem, fruit and leaves. Because of its proteolytic activity, bromelain has potential applications in the cosmetic, pharmaceutical, and food industries. The present study focused on the recovery of bromelain from pineapple peel by liquid–liquid extraction in aqueous two‐phase micellar systems (ATPMS), using Triton X‐114 (TX‐114) and McIlvaine buffer, in the absence and presence of electrolytes CaCl2 and KI; the cloud points of the generated extraction systems were studied by plotting binodal curves. Based on the cloud points, three temperatures were selected for extraction: 30, 33, and 36°C for systems in the absence of salts; 40, 43, and 46°C in the presence of KI; 24, 27, and 30°C in the presence of CaCl2. Total protein and enzymatic activities were analyzed to monitor bromelain. Employing the ATPMS chosen for extraction (0.5 M KI with 3% TX‐114, at pH 6.0, at 40°C), the bromelain extract stability was assessed after incorporation into three cosmetic bases: an anhydrous gel, a cream, and a cream‐gel formulation. The cream‐gel formulation presented as the most appropriate base to convey bromelain, and its optimal storage conditions were found to be 4.0 ± 0.5°C. The selected ATPMS enabled the extraction of a biomolecule with high added value from waste lined‐up in a cosmetic formulation, allowing for exploration of further cosmetic potential. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 31:937–945, 2015</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>25919128</pmid><doi>10.1002/btpr.2098</doi><tpages>9</tpages></addata></record> |
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subjects | Ananas - enzymology Ananas comosus Biotechnology bromelain Bromelains - chemistry Bromelains - isolation & purification Chemical Fractionation - methods cosmetic stability Cosmetics - chemistry enzyme purification liquid-liquid extraction Micelles protein partition |
title | Application of an aqueous two-phase micellar system to extract bromelain from pineapple (Ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations |
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