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Environmental pollutants directly affect the liver X receptor alpha activity: Kinetic and thermodynamic characterization of binding
•Fresh waters pollutants target the oxysterol-binding domain of LXRα.•Kinetic and thermodynamic analysis reveals a drug-like potency by these molecules.•These pollutants affect the LXRα-mediated nuclear transcriptional activity. Liver X receptor is a ligand-activated transcription factor, which is m...
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| Published in: | The Journal of steroid biochemistry and molecular biology 2015-08, Vol.152, p.1-7 |
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| container_title | The Journal of steroid biochemistry and molecular biology |
| container_volume | 152 |
| creator | Mozzicafreddo, Matteo Cuccioloni, Massimiliano Bonfili, Laura Cecarini, Valentina Palermo, Francesco Alessandro Cocci, Paolo Mosconi, Gilberto Capone, Aida Ricci, Irene Eleuteri, Anna Maria Angeletti, Mauro |
| description | •Fresh waters pollutants target the oxysterol-binding domain of LXRα.•Kinetic and thermodynamic analysis reveals a drug-like potency by these molecules.•These pollutants affect the LXRα-mediated nuclear transcriptional activity.
Liver X receptor is a ligand-activated transcription factor, which is mainly involved in cholesterol homeostasis, bile acid and triglycerides metabolism, and, as recently discovered, in the glucose metabolism by direct regulation of liver glucokinase. Its modulation by exogenous factors, such as drugs, industrial by-products, and chemicals is documented. Owing to the abundance of these synthetic molecules in the environment, and to the established target role of this receptor, a number of representative compounds of phthalate, organophosphate and fibrate classes were tested as ligands/modulators of human liver X receptor, using an integrated approach, combining an in silico molecular docking technique with an optical SPR biosensor binding study. The compounds of interest were predicted and proved to target the oxysterols-binding site of human LXRα with measurable binding kinetic constants and with affinities ranging between 4.3×10−7 and 4.3×10−8M. Additionally, non-cytotoxic concentration of these chemicals induced relevant changes in the LXRα gene expression levels and other target genes (SREBP-1c and LGK) in human liver hepatocellular carcinoma cell line (HepG2), as demonstrated by q-RT-PCR. |
| doi_str_mv | 10.1016/j.jsbmb.2015.04.011 |
| format | article |
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Liver X receptor is a ligand-activated transcription factor, which is mainly involved in cholesterol homeostasis, bile acid and triglycerides metabolism, and, as recently discovered, in the glucose metabolism by direct regulation of liver glucokinase. Its modulation by exogenous factors, such as drugs, industrial by-products, and chemicals is documented. Owing to the abundance of these synthetic molecules in the environment, and to the established target role of this receptor, a number of representative compounds of phthalate, organophosphate and fibrate classes were tested as ligands/modulators of human liver X receptor, using an integrated approach, combining an in silico molecular docking technique with an optical SPR biosensor binding study. The compounds of interest were predicted and proved to target the oxysterols-binding site of human LXRα with measurable binding kinetic constants and with affinities ranging between 4.3×10−7 and 4.3×10−8M. Additionally, non-cytotoxic concentration of these chemicals induced relevant changes in the LXRα gene expression levels and other target genes (SREBP-1c and LGK) in human liver hepatocellular carcinoma cell line (HepG2), as demonstrated by q-RT-PCR.</description><identifier>ISSN: 0960-0760</identifier><identifier>EISSN: 1879-1220</identifier><identifier>DOI: 10.1016/j.jsbmb.2015.04.011</identifier><identifier>PMID: 25869557</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Binding Sites ; Binding study ; Cell culture ; Cell Line, Tumor ; Environmental Pollutants - metabolism ; Environmental Pollutants - pharmacology ; Fibric Acids - metabolism ; Fibric Acids - pharmacology ; Hep G2 Cells ; Humans ; Liver X receptor ; Liver X Receptors ; Molecular docking ; Molecular Docking Simulation ; Organophosphates - metabolism ; Organophosphates - pharmacology ; Orphan Nuclear Receptors - biosynthesis ; Orphan Nuclear Receptors - genetics ; Orphan Nuclear Receptors - metabolism ; Phthalic Acids - metabolism ; Phthalic Acids - pharmacology ; Plasticizers ; Protein Binding ; q-RT-PCR ; Receptors, Steroid - metabolism ; RNA, Messenger - biosynthesis ; Sterol Regulatory Element Binding Protein 1 - biosynthesis ; Sterol Regulatory Element Binding Protein 1 - genetics ; Sterol Regulatory Element Binding Protein 1 - metabolism</subject><ispartof>The Journal of steroid biochemistry and molecular biology, 2015-08, Vol.152, p.1-7</ispartof><rights>2015 Elsevier Ltd</rights><rights>Copyright © 2015 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-9951dcafd7c86889942abe497c950e05600714a6397c22e5fd179293d3dd0b073</citedby><cites>FETCH-LOGICAL-c425t-9951dcafd7c86889942abe497c950e05600714a6397c22e5fd179293d3dd0b073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25869557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mozzicafreddo, Matteo</creatorcontrib><creatorcontrib>Cuccioloni, Massimiliano</creatorcontrib><creatorcontrib>Bonfili, Laura</creatorcontrib><creatorcontrib>Cecarini, Valentina</creatorcontrib><creatorcontrib>Palermo, Francesco Alessandro</creatorcontrib><creatorcontrib>Cocci, Paolo</creatorcontrib><creatorcontrib>Mosconi, Gilberto</creatorcontrib><creatorcontrib>Capone, Aida</creatorcontrib><creatorcontrib>Ricci, Irene</creatorcontrib><creatorcontrib>Eleuteri, Anna Maria</creatorcontrib><creatorcontrib>Angeletti, Mauro</creatorcontrib><title>Environmental pollutants directly affect the liver X receptor alpha activity: Kinetic and thermodynamic characterization of binding</title><title>The Journal of steroid biochemistry and molecular biology</title><addtitle>J Steroid Biochem Mol Biol</addtitle><description>•Fresh waters pollutants target the oxysterol-binding domain of LXRα.•Kinetic and thermodynamic analysis reveals a drug-like potency by these molecules.•These pollutants affect the LXRα-mediated nuclear transcriptional activity.
Liver X receptor is a ligand-activated transcription factor, which is mainly involved in cholesterol homeostasis, bile acid and triglycerides metabolism, and, as recently discovered, in the glucose metabolism by direct regulation of liver glucokinase. Its modulation by exogenous factors, such as drugs, industrial by-products, and chemicals is documented. Owing to the abundance of these synthetic molecules in the environment, and to the established target role of this receptor, a number of representative compounds of phthalate, organophosphate and fibrate classes were tested as ligands/modulators of human liver X receptor, using an integrated approach, combining an in silico molecular docking technique with an optical SPR biosensor binding study. The compounds of interest were predicted and proved to target the oxysterols-binding site of human LXRα with measurable binding kinetic constants and with affinities ranging between 4.3×10−7 and 4.3×10−8M. Additionally, non-cytotoxic concentration of these chemicals induced relevant changes in the LXRα gene expression levels and other target genes (SREBP-1c and LGK) in human liver hepatocellular carcinoma cell line (HepG2), as demonstrated by q-RT-PCR.</description><subject>Binding Sites</subject><subject>Binding study</subject><subject>Cell culture</subject><subject>Cell Line, Tumor</subject><subject>Environmental Pollutants - metabolism</subject><subject>Environmental Pollutants - pharmacology</subject><subject>Fibric Acids - metabolism</subject><subject>Fibric Acids - pharmacology</subject><subject>Hep G2 Cells</subject><subject>Humans</subject><subject>Liver X receptor</subject><subject>Liver X Receptors</subject><subject>Molecular docking</subject><subject>Molecular Docking Simulation</subject><subject>Organophosphates - metabolism</subject><subject>Organophosphates - pharmacology</subject><subject>Orphan Nuclear Receptors - biosynthesis</subject><subject>Orphan Nuclear Receptors - genetics</subject><subject>Orphan Nuclear Receptors - metabolism</subject><subject>Phthalic Acids - metabolism</subject><subject>Phthalic Acids - pharmacology</subject><subject>Plasticizers</subject><subject>Protein Binding</subject><subject>q-RT-PCR</subject><subject>Receptors, Steroid - metabolism</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Sterol Regulatory Element Binding Protein 1 - biosynthesis</subject><subject>Sterol Regulatory Element Binding Protein 1 - genetics</subject><subject>Sterol Regulatory Element Binding Protein 1 - metabolism</subject><issn>0960-0760</issn><issn>1879-1220</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNp9kMFu1DAQhi0EotvCEyAhH7kkjJM4iZE4oKoURCUuReJmOfaE9cqxg-1dabn2xfGyhSMnj2a-36P5CHnFoGbA-re7epemZaobYLyGrgbGnpANGwdRsaaBp2QDoocKhh4uyGVKOwBoWzY8JxcNH3vB-bAhDzf-YGPwC_qsHF2Dc_usfE7U2Ig6uyNV81wKmrdInT1gpN9pmeCaQ6TKrVtFlc72YPPxHf1iPWarqfLmFIhLMEevltLRWxULh9H-UtkGT8NMJ-uN9T9ekGezcglfPr5X5NvHm_vrT9Xd19vP1x_uKt01PFdCcGa0ms2gx34chegaNWEnBi04IPAeYGCd6tvSaRrks2GDaERrWmNggqG9Im_O_64x_NxjynKxSaNzymPYJ8l60TPRtSMUtD2jOoaUIs5yjXZR8SgZyJN9uZN_7MuTfQmdLPZL6vXjgv20oPmX-au7AO_PAJYzDxajTNqi13iWLU2w_13wG41FmWk</recordid><startdate>201508</startdate><enddate>201508</enddate><creator>Mozzicafreddo, Matteo</creator><creator>Cuccioloni, Massimiliano</creator><creator>Bonfili, Laura</creator><creator>Cecarini, Valentina</creator><creator>Palermo, Francesco Alessandro</creator><creator>Cocci, Paolo</creator><creator>Mosconi, Gilberto</creator><creator>Capone, Aida</creator><creator>Ricci, Irene</creator><creator>Eleuteri, Anna Maria</creator><creator>Angeletti, Mauro</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201508</creationdate><title>Environmental pollutants directly affect the liver X receptor alpha activity: Kinetic and thermodynamic characterization of binding</title><author>Mozzicafreddo, Matteo ; Cuccioloni, Massimiliano ; Bonfili, Laura ; Cecarini, Valentina ; Palermo, Francesco Alessandro ; Cocci, Paolo ; Mosconi, Gilberto ; Capone, Aida ; Ricci, Irene ; Eleuteri, Anna Maria ; Angeletti, Mauro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-9951dcafd7c86889942abe497c950e05600714a6397c22e5fd179293d3dd0b073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Binding Sites</topic><topic>Binding study</topic><topic>Cell culture</topic><topic>Cell Line, Tumor</topic><topic>Environmental Pollutants - metabolism</topic><topic>Environmental Pollutants - pharmacology</topic><topic>Fibric Acids - metabolism</topic><topic>Fibric Acids - pharmacology</topic><topic>Hep G2 Cells</topic><topic>Humans</topic><topic>Liver X receptor</topic><topic>Liver X Receptors</topic><topic>Molecular docking</topic><topic>Molecular Docking Simulation</topic><topic>Organophosphates - metabolism</topic><topic>Organophosphates - pharmacology</topic><topic>Orphan Nuclear Receptors - biosynthesis</topic><topic>Orphan Nuclear Receptors - genetics</topic><topic>Orphan Nuclear Receptors - metabolism</topic><topic>Phthalic Acids - metabolism</topic><topic>Phthalic Acids - pharmacology</topic><topic>Plasticizers</topic><topic>Protein Binding</topic><topic>q-RT-PCR</topic><topic>Receptors, Steroid - metabolism</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Sterol Regulatory Element Binding Protein 1 - biosynthesis</topic><topic>Sterol Regulatory Element Binding Protein 1 - genetics</topic><topic>Sterol Regulatory Element Binding Protein 1 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mozzicafreddo, Matteo</creatorcontrib><creatorcontrib>Cuccioloni, Massimiliano</creatorcontrib><creatorcontrib>Bonfili, Laura</creatorcontrib><creatorcontrib>Cecarini, Valentina</creatorcontrib><creatorcontrib>Palermo, Francesco Alessandro</creatorcontrib><creatorcontrib>Cocci, Paolo</creatorcontrib><creatorcontrib>Mosconi, Gilberto</creatorcontrib><creatorcontrib>Capone, Aida</creatorcontrib><creatorcontrib>Ricci, Irene</creatorcontrib><creatorcontrib>Eleuteri, Anna Maria</creatorcontrib><creatorcontrib>Angeletti, Mauro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mozzicafreddo, Matteo</au><au>Cuccioloni, Massimiliano</au><au>Bonfili, Laura</au><au>Cecarini, Valentina</au><au>Palermo, Francesco Alessandro</au><au>Cocci, Paolo</au><au>Mosconi, Gilberto</au><au>Capone, Aida</au><au>Ricci, Irene</au><au>Eleuteri, Anna Maria</au><au>Angeletti, Mauro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Environmental pollutants directly affect the liver X receptor alpha activity: Kinetic and thermodynamic characterization of binding</atitle><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle><addtitle>J Steroid Biochem Mol Biol</addtitle><date>2015-08</date><risdate>2015</risdate><volume>152</volume><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>0960-0760</issn><eissn>1879-1220</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>•Fresh waters pollutants target the oxysterol-binding domain of LXRα.•Kinetic and thermodynamic analysis reveals a drug-like potency by these molecules.•These pollutants affect the LXRα-mediated nuclear transcriptional activity.
Liver X receptor is a ligand-activated transcription factor, which is mainly involved in cholesterol homeostasis, bile acid and triglycerides metabolism, and, as recently discovered, in the glucose metabolism by direct regulation of liver glucokinase. Its modulation by exogenous factors, such as drugs, industrial by-products, and chemicals is documented. Owing to the abundance of these synthetic molecules in the environment, and to the established target role of this receptor, a number of representative compounds of phthalate, organophosphate and fibrate classes were tested as ligands/modulators of human liver X receptor, using an integrated approach, combining an in silico molecular docking technique with an optical SPR biosensor binding study. The compounds of interest were predicted and proved to target the oxysterols-binding site of human LXRα with measurable binding kinetic constants and with affinities ranging between 4.3×10−7 and 4.3×10−8M. Additionally, non-cytotoxic concentration of these chemicals induced relevant changes in the LXRα gene expression levels and other target genes (SREBP-1c and LGK) in human liver hepatocellular carcinoma cell line (HepG2), as demonstrated by q-RT-PCR.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>25869557</pmid><doi>10.1016/j.jsbmb.2015.04.011</doi><tpages>7</tpages></addata></record> |
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| source | ScienceDirect Freedom Collection |
| subjects | Binding Sites Binding study Cell culture Cell Line, Tumor Environmental Pollutants - metabolism Environmental Pollutants - pharmacology Fibric Acids - metabolism Fibric Acids - pharmacology Hep G2 Cells Humans Liver X receptor Liver X Receptors Molecular docking Molecular Docking Simulation Organophosphates - metabolism Organophosphates - pharmacology Orphan Nuclear Receptors - biosynthesis Orphan Nuclear Receptors - genetics Orphan Nuclear Receptors - metabolism Phthalic Acids - metabolism Phthalic Acids - pharmacology Plasticizers Protein Binding q-RT-PCR Receptors, Steroid - metabolism RNA, Messenger - biosynthesis Sterol Regulatory Element Binding Protein 1 - biosynthesis Sterol Regulatory Element Binding Protein 1 - genetics Sterol Regulatory Element Binding Protein 1 - metabolism |
| title | Environmental pollutants directly affect the liver X receptor alpha activity: Kinetic and thermodynamic characterization of binding |
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