Temporal variation in the deposition of different types of collagen within a porous biomaterial implant

The deposition of new collagen in association with a medical implant has been studied using expanded polytetrafluoroethylene vascular replacement samples implanted subcutaneously in sheep, for up to 28 days. New type I collagen mRNA synthesis was followed by in situ hybridization, while the accumula...

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Published in:Journal of biomedical materials research. Part A 2014-10, Vol.102 (10), p.3550-3555
Main Authors: White, Jacinta F., Werkmeister, Jerome A., Bisucci, Teresa, Darby, Ian A., Ramshaw, John A. M.
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials - pharmacology
Biomedical materials
cell
Cellular
collagen
Collagen - metabolism
Collagens
Deposition
Enzymes
Immunohistochemistry
Implants, Experimental
In Situ Hybridization
Interstitials
material interaction
Polytetrafluoroethylene - pharmacology
Polytetrafluoroethylenes
Porosity
porous material
Sheep
Staining and Labeling
Surgical implants
Time Factors
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container_issue 10
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container_title Journal of biomedical materials research. Part A
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creator White, Jacinta F.
Werkmeister, Jerome A.
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Ramshaw, John A. M.
description The deposition of new collagen in association with a medical implant has been studied using expanded polytetrafluoroethylene vascular replacement samples implanted subcutaneously in sheep, for up to 28 days. New type I collagen mRNA synthesis was followed by in situ hybridization, while the accumulation of new collagen types III, V, VI, XII, and XIV was followed by immunohistochemistry. All the collagen detected in the pores of the implant were newly deposited at various times after implantation and were not due to any pre‐existing dermal collagen that may have been present around the implant. Collagen deposition was seen initially surrounding the implant and, with time, was seen to infiltrate within its pores. In situ hybridization showed that the majority of infiltrating cells had switched on mRNA that coded for type I collagen production. Histology showed that cellular infiltration increased with time, accompanied by increasing collagen deposition. The deposition of different collagen types happened at different rates. The type V and VI collagens preceded the major interstitial collagens in the newly deposited tissue, although at longer time points, detection of type V collagen appeared to decrease. After disruption of the interstitial collagens with enzyme, the “masked” type V collagen was clearly still visible by immunohistochemistry. Little type XII collagen could be seen within the porous mesh, although it was seen in the surrounding tissues. By contrast, type XIV was seen throughout the porous structure of the implanted mesh, with less being visible outside the material where type XII was more abundant. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3550–3555, 2014.
doi_str_mv 10.1002/jbm.a.35027
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source Wiley Online Library
subjects Animals
Biocompatible Materials - pharmacology
Biomedical materials
cell
Cellular
collagen
Collagen - metabolism
Collagens
Deposition
Enzymes
Immunohistochemistry
Implants, Experimental
In Situ Hybridization
Interstitials
material interaction
Polytetrafluoroethylene - pharmacology
Polytetrafluoroethylenes
Porosity
porous material
Sheep
Staining and Labeling
Surgical implants
Time Factors
title Temporal variation in the deposition of different types of collagen within a porous biomaterial implant
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