Loading…
Molecular Analysis of the Essential and Nonessential Genetic Elements in the Genome of Peanut Chlorotic Streak Caulimovirus
The DNA genome of caulimoviruses contains a set of essential genes: I (movement gene), IV (major capsid protein gene), V (reverse transcriptase gene), and VI (gene coding for a post-transcriptional activator of the expression of other virus genes). In peanut chlorotic streak caulimovirus (PCISV), th...
Saved in:
| Published in: | Virology (New York, N.Y.) N.Y.), 1995-02, Vol.206 (2), p.823-834 |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c410t-34638e353f3a9a5012af76f0789caff9d06c0af6d1c096c219d0445ce110ceed3 |
|---|---|
| cites | |
| container_end_page | 834 |
| container_issue | 2 |
| container_start_page | 823 |
| container_title | Virology (New York, N.Y.) |
| container_volume | 206 |
| creator | Mushegian, A.R. Wolff, J.A. Richins, R.D. Shepherd, R.J. |
| description | The DNA genome of caulimoviruses contains a set of essential genes: I (movement gene), IV (major capsid protein gene), V (reverse transcriptase gene), and VI (gene coding for a post-transcriptional activator of the expression of other virus genes). In peanut chlorotic streak caulimovirus (PCISV), three ORFs, A, B, and C, are located between genes I and IV. They are dissimilar to other caulimovirus ORFs. ORF VII of PCISV is a homolog of ORF VII of soybean chlorotic mottle caulimovirus (SoCMV), but is not similar to the nonconserved ORF VII in other caulimoviruses. The sequence complementary to a portion of tRNAMet, thought to be essential for the priming of minus-strand DNA synthesis in caulimoviruses, is located within the coding sequence of ORF A. To explore the functional significance of ORFs VII, A, B, and C, various mutations were engineered into an infectious DNA clone of PCISV. ORFs VII and B are shown to be dispensable, while ORFs A and C are essential. ORF C is a possible functional equivalent of gene III in other caulimoviruses. Sequences within ORF A that are required for efficient priming of minus-strand synthesis are likely to extend beyond the 12-bp tRNA-binding site. Complete deletion of ORF VII was correlated with severe symptoms, notably with the necrosis of apical meristems. Significance of these observations for the understanding of replication and pathogenesis of plant pararetroviruses and for the improvement of caulimovirus-based expression vectors is discussed. |
| doi_str_mv | 10.1006/viro.1995.1005 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_16764828</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0042682285710057</els_id><sourcerecordid>16764828</sourcerecordid><originalsourceid>FETCH-LOGICAL-c410t-34638e353f3a9a5012af76f0789caff9d06c0af6d1c096c219d0445ce110ceed3</originalsourceid><addsrcrecordid>eNp1UEtLAzEQDqLUWr16E3LytjXZR3b3KKVWoT5APYeYndBoNqnJbqH4583a0pun4XvMx8yH0CUlU0oIu9lo76a0rosBFkdoTEnNEpLl9BiNCcnThFVpeorOQvgkEZclGaFRWWS0puUY_Tw6A7I3wuNbK8w26ICdwt0K8DwEsJ0WBgvb4Cdn4UAswEKnJZ4baCMVsLZ_K5F3LQwBLyBs3-HZyjjvButr50F84ZnojW5dvLoP5-hECRPgYj8n6P1u_ja7T5bPi4fZ7TKROSVdkuUsqyArMpWJWhSEpkKVTJGyqqVQqm4Ik0Qo1lAZX5cpjUyeFxIoJRKgySboepe79u67h9DxVgcJxggLrg-cspLlVVpF43RnlN6F4EHxtdet8FtOCR_a5kPbfGh7gEVcuNon9x8tNAf7vt6oVzsd4nsbDZ4HqcFKaLQH2fHG6f-ifwFMqJBl</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16764828</pqid></control><display><type>article</type><title>Molecular Analysis of the Essential and Nonessential Genetic Elements in the Genome of Peanut Chlorotic Streak Caulimovirus</title><source>BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS</source><source>ScienceDirect Journals</source><creator>Mushegian, A.R. ; Wolff, J.A. ; Richins, R.D. ; Shepherd, R.J.</creator><creatorcontrib>Mushegian, A.R. ; Wolff, J.A. ; Richins, R.D. ; Shepherd, R.J.</creatorcontrib><description>The DNA genome of caulimoviruses contains a set of essential genes: I (movement gene), IV (major capsid protein gene), V (reverse transcriptase gene), and VI (gene coding for a post-transcriptional activator of the expression of other virus genes). In peanut chlorotic streak caulimovirus (PCISV), three ORFs, A, B, and C, are located between genes I and IV. They are dissimilar to other caulimovirus ORFs. ORF VII of PCISV is a homolog of ORF VII of soybean chlorotic mottle caulimovirus (SoCMV), but is not similar to the nonconserved ORF VII in other caulimoviruses. The sequence complementary to a portion of tRNAMet, thought to be essential for the priming of minus-strand DNA synthesis in caulimoviruses, is located within the coding sequence of ORF A. To explore the functional significance of ORFs VII, A, B, and C, various mutations were engineered into an infectious DNA clone of PCISV. ORFs VII and B are shown to be dispensable, while ORFs A and C are essential. ORF C is a possible functional equivalent of gene III in other caulimoviruses. Sequences within ORF A that are required for efficient priming of minus-strand synthesis are likely to extend beyond the 12-bp tRNA-binding site. Complete deletion of ORF VII was correlated with severe symptoms, notably with the necrosis of apical meristems. Significance of these observations for the understanding of replication and pathogenesis of plant pararetroviruses and for the improvement of caulimovirus-based expression vectors is discussed.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1006/viro.1995.1005</identifier><identifier>PMID: 7531917</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Arachis - virology ; Base Sequence ; Capsid - genetics ; Caulimovirus - genetics ; Caulimovirus - metabolism ; Caulimovirus - pathogenicity ; Cloning, Molecular ; DNA Primers ; DNA, Viral - genetics ; DNA, Viral - isolation & purification ; Genes, Lethal ; Genome, Viral ; Molecular Sequence Data ; Mutagenesis ; Mutagenesis, Insertional ; Open Reading Frames ; peanut chlorotic streak virus ; Plant Diseases ; Plasmids ; Polymerase Chain Reaction ; Restriction Mapping ; RNA-Directed DNA Polymerase - genetics ; Sequence Deletion ; Trans-Activators - genetics ; Virion - genetics</subject><ispartof>Virology (New York, N.Y.), 1995-02, Vol.206 (2), p.823-834</ispartof><rights>1995 Academic Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-34638e353f3a9a5012af76f0789caff9d06c0af6d1c096c219d0445ce110ceed3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7531917$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mushegian, A.R.</creatorcontrib><creatorcontrib>Wolff, J.A.</creatorcontrib><creatorcontrib>Richins, R.D.</creatorcontrib><creatorcontrib>Shepherd, R.J.</creatorcontrib><title>Molecular Analysis of the Essential and Nonessential Genetic Elements in the Genome of Peanut Chlorotic Streak Caulimovirus</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>The DNA genome of caulimoviruses contains a set of essential genes: I (movement gene), IV (major capsid protein gene), V (reverse transcriptase gene), and VI (gene coding for a post-transcriptional activator of the expression of other virus genes). In peanut chlorotic streak caulimovirus (PCISV), three ORFs, A, B, and C, are located between genes I and IV. They are dissimilar to other caulimovirus ORFs. ORF VII of PCISV is a homolog of ORF VII of soybean chlorotic mottle caulimovirus (SoCMV), but is not similar to the nonconserved ORF VII in other caulimoviruses. The sequence complementary to a portion of tRNAMet, thought to be essential for the priming of minus-strand DNA synthesis in caulimoviruses, is located within the coding sequence of ORF A. To explore the functional significance of ORFs VII, A, B, and C, various mutations were engineered into an infectious DNA clone of PCISV. ORFs VII and B are shown to be dispensable, while ORFs A and C are essential. ORF C is a possible functional equivalent of gene III in other caulimoviruses. Sequences within ORF A that are required for efficient priming of minus-strand synthesis are likely to extend beyond the 12-bp tRNA-binding site. Complete deletion of ORF VII was correlated with severe symptoms, notably with the necrosis of apical meristems. Significance of these observations for the understanding of replication and pathogenesis of plant pararetroviruses and for the improvement of caulimovirus-based expression vectors is discussed.</description><subject>Arachis - virology</subject><subject>Base Sequence</subject><subject>Capsid - genetics</subject><subject>Caulimovirus - genetics</subject><subject>Caulimovirus - metabolism</subject><subject>Caulimovirus - pathogenicity</subject><subject>Cloning, Molecular</subject><subject>DNA Primers</subject><subject>DNA, Viral - genetics</subject><subject>DNA, Viral - isolation & purification</subject><subject>Genes, Lethal</subject><subject>Genome, Viral</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis</subject><subject>Mutagenesis, Insertional</subject><subject>Open Reading Frames</subject><subject>peanut chlorotic streak virus</subject><subject>Plant Diseases</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Restriction Mapping</subject><subject>RNA-Directed DNA Polymerase - genetics</subject><subject>Sequence Deletion</subject><subject>Trans-Activators - genetics</subject><subject>Virion - genetics</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNp1UEtLAzEQDqLUWr16E3LytjXZR3b3KKVWoT5APYeYndBoNqnJbqH4583a0pun4XvMx8yH0CUlU0oIu9lo76a0rosBFkdoTEnNEpLl9BiNCcnThFVpeorOQvgkEZclGaFRWWS0puUY_Tw6A7I3wuNbK8w26ICdwt0K8DwEsJ0WBgvb4Cdn4UAswEKnJZ4baCMVsLZ_K5F3LQwBLyBs3-HZyjjvButr50F84ZnojW5dvLoP5-hECRPgYj8n6P1u_ja7T5bPi4fZ7TKROSVdkuUsqyArMpWJWhSEpkKVTJGyqqVQqm4Ik0Qo1lAZX5cpjUyeFxIoJRKgySboepe79u67h9DxVgcJxggLrg-cspLlVVpF43RnlN6F4EHxtdet8FtOCR_a5kPbfGh7gEVcuNon9x8tNAf7vt6oVzsd4nsbDZ4HqcFKaLQH2fHG6f-ifwFMqJBl</recordid><startdate>19950201</startdate><enddate>19950201</enddate><creator>Mushegian, A.R.</creator><creator>Wolff, J.A.</creator><creator>Richins, R.D.</creator><creator>Shepherd, R.J.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19950201</creationdate><title>Molecular Analysis of the Essential and Nonessential Genetic Elements in the Genome of Peanut Chlorotic Streak Caulimovirus</title><author>Mushegian, A.R. ; Wolff, J.A. ; Richins, R.D. ; Shepherd, R.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-34638e353f3a9a5012af76f0789caff9d06c0af6d1c096c219d0445ce110ceed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Arachis - virology</topic><topic>Base Sequence</topic><topic>Capsid - genetics</topic><topic>Caulimovirus - genetics</topic><topic>Caulimovirus - metabolism</topic><topic>Caulimovirus - pathogenicity</topic><topic>Cloning, Molecular</topic><topic>DNA Primers</topic><topic>DNA, Viral - genetics</topic><topic>DNA, Viral - isolation & purification</topic><topic>Genes, Lethal</topic><topic>Genome, Viral</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis</topic><topic>Mutagenesis, Insertional</topic><topic>Open Reading Frames</topic><topic>peanut chlorotic streak virus</topic><topic>Plant Diseases</topic><topic>Plasmids</topic><topic>Polymerase Chain Reaction</topic><topic>Restriction Mapping</topic><topic>RNA-Directed DNA Polymerase - genetics</topic><topic>Sequence Deletion</topic><topic>Trans-Activators - genetics</topic><topic>Virion - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mushegian, A.R.</creatorcontrib><creatorcontrib>Wolff, J.A.</creatorcontrib><creatorcontrib>Richins, R.D.</creatorcontrib><creatorcontrib>Shepherd, R.J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mushegian, A.R.</au><au>Wolff, J.A.</au><au>Richins, R.D.</au><au>Shepherd, R.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Analysis of the Essential and Nonessential Genetic Elements in the Genome of Peanut Chlorotic Streak Caulimovirus</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1995-02-01</date><risdate>1995</risdate><volume>206</volume><issue>2</issue><spage>823</spage><epage>834</epage><pages>823-834</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><abstract>The DNA genome of caulimoviruses contains a set of essential genes: I (movement gene), IV (major capsid protein gene), V (reverse transcriptase gene), and VI (gene coding for a post-transcriptional activator of the expression of other virus genes). In peanut chlorotic streak caulimovirus (PCISV), three ORFs, A, B, and C, are located between genes I and IV. They are dissimilar to other caulimovirus ORFs. ORF VII of PCISV is a homolog of ORF VII of soybean chlorotic mottle caulimovirus (SoCMV), but is not similar to the nonconserved ORF VII in other caulimoviruses. The sequence complementary to a portion of tRNAMet, thought to be essential for the priming of minus-strand DNA synthesis in caulimoviruses, is located within the coding sequence of ORF A. To explore the functional significance of ORFs VII, A, B, and C, various mutations were engineered into an infectious DNA clone of PCISV. ORFs VII and B are shown to be dispensable, while ORFs A and C are essential. ORF C is a possible functional equivalent of gene III in other caulimoviruses. Sequences within ORF A that are required for efficient priming of minus-strand synthesis are likely to extend beyond the 12-bp tRNA-binding site. Complete deletion of ORF VII was correlated with severe symptoms, notably with the necrosis of apical meristems. Significance of these observations for the understanding of replication and pathogenesis of plant pararetroviruses and for the improvement of caulimovirus-based expression vectors is discussed.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7531917</pmid><doi>10.1006/viro.1995.1005</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1995-02, Vol.206 (2), p.823-834 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16764828 |
| source | BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS; ScienceDirect Journals |
| subjects | Arachis - virology Base Sequence Capsid - genetics Caulimovirus - genetics Caulimovirus - metabolism Caulimovirus - pathogenicity Cloning, Molecular DNA Primers DNA, Viral - genetics DNA, Viral - isolation & purification Genes, Lethal Genome, Viral Molecular Sequence Data Mutagenesis Mutagenesis, Insertional Open Reading Frames peanut chlorotic streak virus Plant Diseases Plasmids Polymerase Chain Reaction Restriction Mapping RNA-Directed DNA Polymerase - genetics Sequence Deletion Trans-Activators - genetics Virion - genetics |
| title | Molecular Analysis of the Essential and Nonessential Genetic Elements in the Genome of Peanut Chlorotic Streak Caulimovirus |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T03%3A06%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20Analysis%20of%20the%20Essential%20and%20Nonessential%20Genetic%20Elements%20in%20the%20Genome%20of%20Peanut%20Chlorotic%20Streak%20Caulimovirus&rft.jtitle=Virology%20(New%20York,%20N.Y.)&rft.au=Mushegian,%20A.R.&rft.date=1995-02-01&rft.volume=206&rft.issue=2&rft.spage=823&rft.epage=834&rft.pages=823-834&rft.issn=0042-6822&rft.eissn=1096-0341&rft_id=info:doi/10.1006/viro.1995.1005&rft_dat=%3Cproquest_cross%3E16764828%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c410t-34638e353f3a9a5012af76f0789caff9d06c0af6d1c096c219d0445ce110ceed3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=16764828&rft_id=info:pmid/7531917&rfr_iscdi=true |