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DORSAL TWIST INTERACTIONS ESTABLISH SNAIL EXPRESSION IN THE PRESUMPTIVE MESODERM OF THE DROSOPHILA EMBRYO
The first step in the differentiation of the Drosophila mesoderm is the activation of two regulatory genes, twist (twi) and snail (sna), in ventral regions of early embryos. sna is a transcriptional repressor that is uniformly expressed throughout the presumptive mesoderm. Its sharp lateral limits h...
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| Published in: | Genes & development 1992-08, Vol.6 (8), p.1518-1530 |
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| Main Authors: | , , , , |
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| Language: | English |
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| container_end_page | 1530 |
| container_issue | 8 |
| container_start_page | 1518 |
| container_title | Genes & development |
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| creator | IP, YT PARK, RE KOSMAN, D YAZDANBAKHSH, K LEVINE, M |
| description | The first step in the differentiation of the Drosophila mesoderm is the activation of two regulatory genes, twist (twi) and snail (sna), in ventral regions of early embryos. sna is a transcriptional repressor that is uniformly expressed throughout the presumptive mesoderm. Its sharp lateral limits help to establish the boundary between the mesoderm and neuroectoderm. Genetic studies suggest that sna is a target of the dorsal (dl) morphogen, and this interaction provides a model for determining how a morphogen gradient establishes a sharp, on/off threshold response. We present evidence that dl and twi directly activate sna expression. Site-directed mutagenesis of dl- and twi-binding sites within defined regions of the sna promoter suggest that the two proteins (containing the Rel and helix-loop-helix domains, respectively) function multiplicatively to ensure strong, uniform expression of sna, particularly in ventral-lateral regions where there are diminishing amounts of dl. These results are consistent with the possibility that the sharp sna borders are formed by multiplying the shallow dl gradient and the steeper twi gradient. |
| doi_str_mv | 10.1101/gad.6.8.1518 |
| format | article |
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Its sharp lateral limits help to establish the boundary between the mesoderm and neuroectoderm. Genetic studies suggest that sna is a target of the dorsal (dl) morphogen, and this interaction provides a model for determining how a morphogen gradient establishes a sharp, on/off threshold response. We present evidence that dl and twi directly activate sna expression. Site-directed mutagenesis of dl- and twi-binding sites within defined regions of the sna promoter suggest that the two proteins (containing the Rel and helix-loop-helix domains, respectively) function multiplicatively to ensure strong, uniform expression of sna, particularly in ventral-lateral regions where there are diminishing amounts of dl. These results are consistent with the possibility that the sharp sna borders are formed by multiplying the shallow dl gradient and the steeper twi gradient.</description><identifier>ISSN: 0890-9369</identifier><identifier>EISSN: 1549-5477</identifier><identifier>DOI: 10.1101/gad.6.8.1518</identifier><identifier>PMID: 1644293</identifier><identifier>CODEN: GEDEEP</identifier><language>eng</language><publisher>PLAINVIEW: COLD SPRING HARBOR LAB PRESS</publisher><subject>Animals ; Base Sequence ; Binding Sites - genetics ; Biological and medical sciences ; Cell Biology ; Cloning, Molecular ; Developmental Biology ; Drosophila ; Drosophila - embryology ; Drosophila - genetics ; Drosophila Proteins ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation - physiology ; Genes, Regulator - genetics ; Genetics & Heredity ; Larva - genetics ; Larva - growth & development ; Life Sciences & Biomedicine ; Mesoderm - metabolism ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; Phosphoproteins ; Plasmids - genetics ; Promoter Regions, Genetic - genetics ; Recombinant Fusion Proteins - genetics ; Repressor Proteins - genetics ; Science & Technology ; Transcription Factors ; Twist-Related Protein 1</subject><ispartof>Genes & development, 1992-08, Vol.6 (8), p.1518-1530</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>277</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wosA1992JH59900013</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c384t-4c38138dc9ab5931654656794a8510068b6345a25c9cacd4698d5fe793ae90333</citedby><cites>FETCH-LOGICAL-c384t-4c38138dc9ab5931654656794a8510068b6345a25c9cacd4698d5fe793ae90333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27225,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5479346$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1644293$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>IP, YT</creatorcontrib><creatorcontrib>PARK, RE</creatorcontrib><creatorcontrib>KOSMAN, D</creatorcontrib><creatorcontrib>YAZDANBAKHSH, K</creatorcontrib><creatorcontrib>LEVINE, M</creatorcontrib><title>DORSAL TWIST INTERACTIONS ESTABLISH SNAIL EXPRESSION IN THE PRESUMPTIVE MESODERM OF THE DROSOPHILA EMBRYO</title><title>Genes & development</title><addtitle>GENE DEV</addtitle><addtitle>Genes Dev</addtitle><description>The first step in the differentiation of the Drosophila mesoderm is the activation of two regulatory genes, twist (twi) and snail (sna), in ventral regions of early embryos. sna is a transcriptional repressor that is uniformly expressed throughout the presumptive mesoderm. Its sharp lateral limits help to establish the boundary between the mesoderm and neuroectoderm. Genetic studies suggest that sna is a target of the dorsal (dl) morphogen, and this interaction provides a model for determining how a morphogen gradient establishes a sharp, on/off threshold response. We present evidence that dl and twi directly activate sna expression. Site-directed mutagenesis of dl- and twi-binding sites within defined regions of the sna promoter suggest that the two proteins (containing the Rel and helix-loop-helix domains, respectively) function multiplicatively to ensure strong, uniform expression of sna, particularly in ventral-lateral regions where there are diminishing amounts of dl. These results are consistent with the possibility that the sharp sna borders are formed by multiplying the shallow dl gradient and the steeper twi gradient.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Binding Sites - genetics</subject><subject>Biological and medical sciences</subject><subject>Cell Biology</subject><subject>Cloning, Molecular</subject><subject>Developmental Biology</subject><subject>Drosophila</subject><subject>Drosophila - embryology</subject><subject>Drosophila - genetics</subject><subject>Drosophila Proteins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - physiology</subject><subject>Genes, Regulator - genetics</subject><subject>Genetics & Heredity</subject><subject>Larva - genetics</subject><subject>Larva - growth & development</subject><subject>Life Sciences & Biomedicine</subject><subject>Mesoderm - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Site-Directed</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Phosphoproteins</subject><subject>Plasmids - genetics</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Repressor Proteins - genetics</subject><subject>Science & Technology</subject><subject>Transcription Factors</subject><subject>Twist-Related Protein 1</subject><issn>0890-9369</issn><issn>1549-5477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EZCTM</sourceid><recordid>eNqNkU2P0zAYhC0EWsrCjSuSD4gLpNj1R-1jNvWSoLSp4iwfp8h1HJRVmyxxKsS_x6XVcuU0ejWPRq9mAHiN0RxjhD_-MM2cz8UcMyyegBlmVEaMLpdPwQwJiSJJuHwOXnh_jxDiiPMrcIU5pQtJZqBbFaWOc1h9zXQFs02lyjipsmKjodJVfJNnOoV6E2c5VN-2pdI6eIGDVarg6b5bb6vsi4JrpYuVKtewuP3rrcpCF9s0y2Oo1jfl9-IleNaavXevLnoN7m5VlaRRXnzKkjiPLBF0imgQTERjpdkxSTBnlDO-lNQIhsP_YscJZWbBrLTGNpRL0bDWLSUxTiJCyDV4d859GIefR-en-tB56_Z707vh6OuQSCRjNIAfzqAdB-9H19YPY3cw4-8ao_rUbB2arXkt6lOzAX9zyT3uDq75B5-rDP7bi2-8Nft2NL3t_CMWFpGE8oC9P2O_3G5ove1cb90jFWMpF59TJmXYCp9Cxf_TSTeZqRv6ZDj2E_kDyXCYHg</recordid><startdate>19920801</startdate><enddate>19920801</enddate><creator>IP, YT</creator><creator>PARK, RE</creator><creator>KOSMAN, D</creator><creator>YAZDANBAKHSH, K</creator><creator>LEVINE, M</creator><general>COLD SPRING HARBOR LAB PRESS</general><general>Cold Spring Harbor Laboratory</general><scope>BLEPL</scope><scope>DTL</scope><scope>EZCTM</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19920801</creationdate><title>DORSAL TWIST INTERACTIONS ESTABLISH SNAIL EXPRESSION IN THE PRESUMPTIVE MESODERM OF THE DROSOPHILA EMBRYO</title><author>IP, YT ; PARK, RE ; KOSMAN, D ; YAZDANBAKHSH, K ; LEVINE, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-4c38138dc9ab5931654656794a8510068b6345a25c9cacd4698d5fe793ae90333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Binding Sites - genetics</topic><topic>Biological and medical sciences</topic><topic>Cell Biology</topic><topic>Cloning, Molecular</topic><topic>Developmental Biology</topic><topic>Drosophila</topic><topic>Drosophila - embryology</topic><topic>Drosophila - genetics</topic><topic>Drosophila Proteins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - physiology</topic><topic>Genes, Regulator - genetics</topic><topic>Genetics & Heredity</topic><topic>Larva - genetics</topic><topic>Larva - growth & development</topic><topic>Life Sciences & Biomedicine</topic><topic>Mesoderm - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Site-Directed</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>Phosphoproteins</topic><topic>Plasmids - genetics</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Repressor Proteins - genetics</topic><topic>Science & Technology</topic><topic>Transcription Factors</topic><topic>Twist-Related Protein 1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>IP, YT</creatorcontrib><creatorcontrib>PARK, RE</creatorcontrib><creatorcontrib>KOSMAN, D</creatorcontrib><creatorcontrib>YAZDANBAKHSH, K</creatorcontrib><creatorcontrib>LEVINE, M</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 1992</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Genes & development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>IP, YT</au><au>PARK, RE</au><au>KOSMAN, D</au><au>YAZDANBAKHSH, K</au><au>LEVINE, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DORSAL TWIST INTERACTIONS ESTABLISH SNAIL EXPRESSION IN THE PRESUMPTIVE MESODERM OF THE DROSOPHILA EMBRYO</atitle><jtitle>Genes & development</jtitle><stitle>GENE DEV</stitle><addtitle>Genes Dev</addtitle><date>1992-08-01</date><risdate>1992</risdate><volume>6</volume><issue>8</issue><spage>1518</spage><epage>1530</epage><pages>1518-1530</pages><issn>0890-9369</issn><eissn>1549-5477</eissn><coden>GEDEEP</coden><notes>Medline</notes><notes>NIH RePORTER</notes><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><abstract>The first step in the differentiation of the Drosophila mesoderm is the activation of two regulatory genes, twist (twi) and snail (sna), in ventral regions of early embryos. sna is a transcriptional repressor that is uniformly expressed throughout the presumptive mesoderm. Its sharp lateral limits help to establish the boundary between the mesoderm and neuroectoderm. Genetic studies suggest that sna is a target of the dorsal (dl) morphogen, and this interaction provides a model for determining how a morphogen gradient establishes a sharp, on/off threshold response. We present evidence that dl and twi directly activate sna expression. Site-directed mutagenesis of dl- and twi-binding sites within defined regions of the sna promoter suggest that the two proteins (containing the Rel and helix-loop-helix domains, respectively) function multiplicatively to ensure strong, uniform expression of sna, particularly in ventral-lateral regions where there are diminishing amounts of dl. These results are consistent with the possibility that the sharp sna borders are formed by multiplying the shallow dl gradient and the steeper twi gradient.</abstract><cop>PLAINVIEW</cop><pub>COLD SPRING HARBOR LAB PRESS</pub><pmid>1644293</pmid><doi>10.1101/gad.6.8.1518</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0890-9369 |
| ispartof | Genes & development, 1992-08, Vol.6 (8), p.1518-1530 |
| issn | 0890-9369 1549-5477 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16539554 |
| source | Web of Science - Science Citation Index Expanded - 1992<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; Freely Accessible Science Journals - check A-Z of ejournals |
| subjects | Animals Base Sequence Binding Sites - genetics Biological and medical sciences Cell Biology Cloning, Molecular Developmental Biology Drosophila Drosophila - embryology Drosophila - genetics Drosophila Proteins Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation - physiology Genes, Regulator - genetics Genetics & Heredity Larva - genetics Larva - growth & development Life Sciences & Biomedicine Mesoderm - metabolism Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutagenesis, Site-Directed Nuclear Proteins - genetics Nuclear Proteins - metabolism Phosphoproteins Plasmids - genetics Promoter Regions, Genetic - genetics Recombinant Fusion Proteins - genetics Repressor Proteins - genetics Science & Technology Transcription Factors Twist-Related Protein 1 |
| title | DORSAL TWIST INTERACTIONS ESTABLISH SNAIL EXPRESSION IN THE PRESUMPTIVE MESODERM OF THE DROSOPHILA EMBRYO |
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