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Wide detection of Aedes flavivirus in north-eastern Italy--a European hotspot of emerging mosquito-borne diseases
The pattern of flavivirus infection in mosquitoes belonging to the genera Aedes and Culex collected in two regions of north-eastern Italy (Trentino and Veneto) was assessed. Mosquitoes were collected during 2012 and screened for flaviviruses using a generic reverse transcription-nested-PCR targeted...
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| Published in: | Journal of general virology 2015-02, Vol.96 (Pt 2), p.420-430 |
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| Main Authors: | , , , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c331t-a58e81ea9c622237854819a8345f2c7ea431ce3a9d8ffde10cd81af9a2645d3e3 |
| container_end_page | 430 |
| container_issue | Pt 2 |
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| container_title | Journal of general virology |
| container_volume | 96 |
| creator | Grisenti, Michela Vázquez, Ana Herrero, Laura Cuevas, Laureano Perez-Pastrana, Esperanza Arnoldi, Daniele Rosà, Roberto Capelli, Gioia Tenorio, Antonio Sánchez-Seco, Maria Paz Rizzoli, Annapaola |
| description | The pattern of flavivirus infection in mosquitoes belonging to the genera Aedes and Culex collected in two regions of north-eastern Italy (Trentino and Veneto) was assessed. Mosquitoes were collected during 2012 and screened for flaviviruses using a generic reverse transcription-nested-PCR targeted on a region of the non-structural NS5 gene. The phylogenetic analysis was performed on a fragment of ~1000 bp. Virus isolation was attempted in C6/36 insect cell lines and the infected cell cultures were studied by electron microscopy. We detected a wide distribution of Aedes flavivirus (AeFV) in Aedes albopictus, with higher infection prevalence in Trentino than in Veneto. In Culex pipiens collected in Veneto, we detected a new sequence of an insect-specific flavivirus and one of Usutu virus. Interestingly, we detected AeFV in C. pipiens, for the first time to our knowledge, in both regions. Viral isolation in cell culture was successful for AeFV. AeFV sequences found in Veneto showed a high percentage of similarity to those detected in Trentino and to those previously reported in other areas of northern Italy. Co-infections with different flaviviruses were not detected. |
| doi_str_mv | 10.1099/vir.0.069625-0 |
| format | article |
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Mosquitoes were collected during 2012 and screened for flaviviruses using a generic reverse transcription-nested-PCR targeted on a region of the non-structural NS5 gene. The phylogenetic analysis was performed on a fragment of ~1000 bp. Virus isolation was attempted in C6/36 insect cell lines and the infected cell cultures were studied by electron microscopy. We detected a wide distribution of Aedes flavivirus (AeFV) in Aedes albopictus, with higher infection prevalence in Trentino than in Veneto. In Culex pipiens collected in Veneto, we detected a new sequence of an insect-specific flavivirus and one of Usutu virus. Interestingly, we detected AeFV in C. pipiens, for the first time to our knowledge, in both regions. Viral isolation in cell culture was successful for AeFV. AeFV sequences found in Veneto showed a high percentage of similarity to those detected in Trentino and to those previously reported in other areas of northern Italy. 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Mosquitoes were collected during 2012 and screened for flaviviruses using a generic reverse transcription-nested-PCR targeted on a region of the non-structural NS5 gene. The phylogenetic analysis was performed on a fragment of ~1000 bp. Virus isolation was attempted in C6/36 insect cell lines and the infected cell cultures were studied by electron microscopy. We detected a wide distribution of Aedes flavivirus (AeFV) in Aedes albopictus, with higher infection prevalence in Trentino than in Veneto. In Culex pipiens collected in Veneto, we detected a new sequence of an insect-specific flavivirus and one of Usutu virus. Interestingly, we detected AeFV in C. pipiens, for the first time to our knowledge, in both regions. Viral isolation in cell culture was successful for AeFV. AeFV sequences found in Veneto showed a high percentage of similarity to those detected in Trentino and to those previously reported in other areas of northern Italy. Co-infections with different flaviviruses were not detected.</description><subject>Aedes - virology</subject><subject>Animals</subject><subject>Cell Line</subject><subject>Culex - virology</subject><subject>Female</subject><subject>Flavivirus - classification</subject><subject>Flavivirus - genetics</subject><subject>Flavivirus - isolation & purification</subject><subject>Flavivirus - ultrastructure</subject><subject>Italy</subject><subject>Male</subject><subject>Microscopy, Electron, Transmission</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Viral Nonstructural Proteins - genetics</subject><subject>Virion - ultrastructure</subject><subject>Virus Cultivation</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNo9kD1PwzAURS0EoqWwMiKPLA7-iJN4rKoClSqxgBgtN3lpjZI4tZ1K_fekamF6w73nPukg9MhowqhSLwfrE5rQTGVcEnqFpizNJOFjdI2mlHJOmGD5BN2F8EMpS1OZ36IJl4Jngokp2n_bCnAFEcpoXYddjedQQcB1Yw52XB8Cth3unI87AiZE8B1eRdMcCTF4OXjXg-nwzsXQu3jCoQW_td0Wty7sBxsd2TjfjT9sGHkI9-imNk2Ah8udoa_X5efinaw_3laL-ZqUQrBIjCygYGBUmXHORV7ItGDKFCKVNS9zMKlgJQijqqKuK2C0rApmamV4lspKgJih5_Nu791-gBB1a0MJTWM6cEPQLJM8pTyXaqwm52rpXQgeat172xp_1Izqk2Y9itBUnzVrOgJPl-1h00L1X__zKn4BX896kw</recordid><startdate>201502</startdate><enddate>201502</enddate><creator>Grisenti, Michela</creator><creator>Vázquez, Ana</creator><creator>Herrero, Laura</creator><creator>Cuevas, Laureano</creator><creator>Perez-Pastrana, Esperanza</creator><creator>Arnoldi, Daniele</creator><creator>Rosà, Roberto</creator><creator>Capelli, Gioia</creator><creator>Tenorio, Antonio</creator><creator>Sánchez-Seco, Maria Paz</creator><creator>Rizzoli, Annapaola</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201502</creationdate><title>Wide detection of Aedes flavivirus in north-eastern Italy--a European hotspot of emerging mosquito-borne diseases</title><author>Grisenti, Michela ; Vázquez, Ana ; Herrero, Laura ; Cuevas, Laureano ; Perez-Pastrana, Esperanza ; Arnoldi, Daniele ; Rosà, Roberto ; Capelli, Gioia ; Tenorio, Antonio ; Sánchez-Seco, Maria Paz ; Rizzoli, Annapaola</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c331t-a58e81ea9c622237854819a8345f2c7ea431ce3a9d8ffde10cd81af9a2645d3e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aedes - virology</topic><topic>Animals</topic><topic>Cell Line</topic><topic>Culex - virology</topic><topic>Female</topic><topic>Flavivirus - classification</topic><topic>Flavivirus - genetics</topic><topic>Flavivirus - isolation & purification</topic><topic>Flavivirus - ultrastructure</topic><topic>Italy</topic><topic>Male</topic><topic>Microscopy, Electron, Transmission</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Viral - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Viral Nonstructural Proteins - genetics</topic><topic>Virion - ultrastructure</topic><topic>Virus Cultivation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grisenti, Michela</creatorcontrib><creatorcontrib>Vázquez, Ana</creatorcontrib><creatorcontrib>Herrero, Laura</creatorcontrib><creatorcontrib>Cuevas, Laureano</creatorcontrib><creatorcontrib>Perez-Pastrana, Esperanza</creatorcontrib><creatorcontrib>Arnoldi, Daniele</creatorcontrib><creatorcontrib>Rosà, Roberto</creatorcontrib><creatorcontrib>Capelli, Gioia</creatorcontrib><creatorcontrib>Tenorio, Antonio</creatorcontrib><creatorcontrib>Sánchez-Seco, Maria Paz</creatorcontrib><creatorcontrib>Rizzoli, Annapaola</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grisenti, Michela</au><au>Vázquez, Ana</au><au>Herrero, Laura</au><au>Cuevas, Laureano</au><au>Perez-Pastrana, Esperanza</au><au>Arnoldi, Daniele</au><au>Rosà, Roberto</au><au>Capelli, Gioia</au><au>Tenorio, Antonio</au><au>Sánchez-Seco, Maria Paz</au><au>Rizzoli, Annapaola</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Wide detection of Aedes flavivirus in north-eastern Italy--a European hotspot of emerging mosquito-borne diseases</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>2015-02</date><risdate>2015</risdate><volume>96</volume><issue>Pt 2</issue><spage>420</spage><epage>430</epage><pages>420-430</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>The pattern of flavivirus infection in mosquitoes belonging to the genera Aedes and Culex collected in two regions of north-eastern Italy (Trentino and Veneto) was assessed. Mosquitoes were collected during 2012 and screened for flaviviruses using a generic reverse transcription-nested-PCR targeted on a region of the non-structural NS5 gene. The phylogenetic analysis was performed on a fragment of ~1000 bp. Virus isolation was attempted in C6/36 insect cell lines and the infected cell cultures were studied by electron microscopy. We detected a wide distribution of Aedes flavivirus (AeFV) in Aedes albopictus, with higher infection prevalence in Trentino than in Veneto. In Culex pipiens collected in Veneto, we detected a new sequence of an insect-specific flavivirus and one of Usutu virus. Interestingly, we detected AeFV in C. pipiens, for the first time to our knowledge, in both regions. Viral isolation in cell culture was successful for AeFV. AeFV sequences found in Veneto showed a high percentage of similarity to those detected in Trentino and to those previously reported in other areas of northern Italy. 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| subjects | Aedes - virology Animals Cell Line Culex - virology Female Flavivirus - classification Flavivirus - genetics Flavivirus - isolation & purification Flavivirus - ultrastructure Italy Male Microscopy, Electron, Transmission Molecular Sequence Data Phylogeny Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - genetics Sequence Analysis, DNA Viral Nonstructural Proteins - genetics Virion - ultrastructure Virus Cultivation |
| title | Wide detection of Aedes flavivirus in north-eastern Italy--a European hotspot of emerging mosquito-borne diseases |
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