A preliminary Raman spectroscopic study of urine: diagnosis of breast cancer in animal models

Prognosis of breast cancer, the most common cancer in females worldwide, has been shown to improve with early detection. Owing to disadvantages like low sensitivity, specificity, tedious sample preparation, long output times and inter-observer variance of currently available screening/diagnostic too...

Full description

Saved in:
Bibliographic Details
Published in:Analyst (London) 2015-01, Vol.14 (2), p.456-466
Main Authors: Bhattacharjee, T, Khan, A, Maru, G, Ingle, A, Krishna, C. Murali
Format: Article
Language:English
Subjects:
Animals
Breast
Cancer
Carcinogens
Diagnosis
Disease Models, Animal
Female
Mammary Neoplasms, Animal - diagnosis
Multivariate Analysis
Principal Component Analysis
Rats
Rats, Sprague-Dawley
Spectra
Spectrum Analysis, Raman
Tumors
Urinalysis - instrumentation
Urinalysis - methods
Urine
Urine - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Prognosis of breast cancer, the most common cancer in females worldwide, has been shown to improve with early detection. Owing to disadvantages like low sensitivity, specificity, tedious sample preparation, long output times and inter-observer variance of currently available screening/diagnostic tools, rapid and objective alternatives such as Raman spectroscopy (RS) are being extensively explored. Body fluid (serum and saliva) based RS assays have shown promising results in diagnosis of oral, lung and nasopharyngeal cancers. The current study aims to explore the feasibility of breast cancer diagnosis using urine based RS. In this study, spectra were acquired from unprocessed as well as concentrated urine of controls (C) and breast tumor bearing (T) rats and analyzed using Principal Component Analysis (PCA) and Principal Component-Linear Discriminant Analysis (PC-LDA). Classification efficiencies of 80% and 72% using unprocessed urine and 78% and 91% using concentrated urine for C and T rats were achieved. Thus, results suggest the possibility of breast cancer diagnosis using urine based RS. Further, spectra were also acquired from concentrated urine samples collected prior to breast tumor development (TT) in rats and from rats that did not develop tumors despite carcinogen treatment (NTT). Concentrated urine of NTT rats could be classified as 'normal' (C or NTT) with ∼83% efficiency whereas concentrated urine from visibly and palpably normal rats that eventually developed tumor (TT rats) could be classified as 'abnormal' (TT or T) with ∼72.5% efficiency using PC-LDA. These results suggest the possibility of detecting biochemical changes occurring prior to tumor development using urine based RS. Analysis of urine by Raman spectroscopy (RS) as an alternative screening and diagnostic tool for breast cancer..
ISSN:0003-2654
1364-5528
DOI:10.1039/c4an01703j